Figure 6 - uploaded by Yasemin Yoluc
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RNA modification and sequence mapping of E. coli tRNA Lys from the digestion products of RNases T1, cusativin, and MC1. (A) The observed digestion products of each ribonuclease are matched against the known sequence of tRNA Lys . The cleavage sites for each enzyme is underlined in an oligomer. Note the overlapping regions between the sequences of digestion products. (B) A schematic view of looking at the overlaps between the observed digestion products of the three ribonuacleases. (C) The clover-leaf model of 2D structure of E. coli tRNA Lys is depicted. Figure reproduced with permission from (Thakur et al. 2020). See colour version of this figure at www.tandfonline.com/ibmg.
Source publication
Organisms from all domains of life invest a substantial amount of energy for the introduction ofRNA modifications into nearly all transcripts studied to date. Instrumental analysis of RNA canfocus on the modified residues and reveal the function of these epitranscriptomic marks. Here,we will review recent advances and breakthroughs achieved by NMR...
Contexts in source publication
Context 1
... the use of single RNases has been proven effective throughout the years, the inherent challenge has been that a single RNase limits the analyst's ability to accurately reconstruct the entire RNA sequence. Not surprisingly then, much of the recent work in this area has focused on identifying and implementing new RNases that can be used in combination to increase sequence coverage through the generation of overlapping digestion products ( Figure 6). ...
Context 2
... the use of single RNases has been proven effective throughout the years, the inherent challenge has been that a single RNase limits the analyst's ability to accurately reconstruct the entire RNA sequence. Not surprisingly then, much of the recent work in this area has focused on identifying and implementing new RNases that can be used in combination to increase sequence coverage through the generation of overlapping digestion products ( Figure 6). ...
