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RAxML tree based on concatenated partial large subunit and partial mitochondrial small subunit sequences from selected Ostropales, based on Baloch et al. (2013). Placopsis and Orceolina (Baeomycetales) selected as the outgroup. Edges with bootstrap values
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Sphaeropezia leucocheila is described as a new species from New Zealand. Known from a single specimen, its fruiting bodies were consistently associated with a patch of dead and dying leaves in a liverwort colony that included several species of Lepidoziaceae. A phylogenetic analysis places this fungus in Sphaeropezia, a genus that includes several...
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... analyses were performed with RAxML 8.2.11 using the GTR model and the BFGS method to optimize GTR rate parameters (Stamatakis 2014), as implemented in Geneious, with support estimated from 1000 bootstrap replications. TABLE 1. Specimens and GenBank accession numbers of sequences used for the phylogeny in Figure 1. All except S. leucocheila are used in the analyses in Baloch et al. 2013. ...
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... DNA sequences from each separate ascoma from PDD 98299 were identical, so confirming that chance, contaminating fungi from the surface of the ascomata were not being accidentally amplified. The topology of the phylogenetic tree ( Fig. 1), based on LSU and mtSSU, matches that from Baloch et al. (2013); their analysis also used RPB2. The genus Sphaeropezia is strongly supported as a monophyletic clade within the Stictidaceae sensu Baloch et al. (2013). The new species described here, S. leucocheila, is basal in this clade. greater than 90% are indicated with thick lines. ...
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... analyses were performed with RAxML 8.2.11 using the GTR model and the BFGS method to optimize GTR rate parameters (Stamatakis 2014), as implemented in Geneious, with support estimated from 1000 bootstrap replications. TABLE 1. Specimens and GenBank accession numbers of sequences used for the phylogeny in Figure 1. All except S. leucocheila are used in the analyses in Baloch et al. 2013. ...
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... DNA sequences from each separate ascoma from PDD 98299 were identical, so confirming that chance, contaminating fungi from the surface of the ascomata were not being accidentally amplified. The topology of the phylogenetic tree ( Fig. 1), based on LSU and mtSSU, matches that from Baloch et al. (2013); their analysis also used RPB2. The genus Sphaeropezia is strongly supported as a monophyletic clade within the Stictidaceae sensu Baloch et al. (2013). The new species described here, S. leucocheila, is basal in this clade. greater than 90% are indicated with thick lines. ...
Citations
... The presence of these characters in all Fitzroyomyces species suggests that they might be useful diagnostic features of this genus. Sphaeropezia clade contains four species, including Sphaeropezia leucocheila (oblong-elliptic, 1-septate) [73], S. lyckselensis (cylindrical oblong, 3-septate) [42], S. mycoblasti (ellipsoidal, 3-septate) [42] and S. shangrilaensis (fusoid to obovoid, aseptate) [20]. The four species form short, nearly cylindrical and few-celled (0-3-septate) ascospores, indicating that the ascospore morphology is consistent and is, thus, a phylogenetically informative character within Sphaeropezia. ...
Stictidaceae comprises taxa with diverse lifestyles. Many species in this family are drought resistant and important for studying fungal adaptation and evolution. Stictidaceae comprises 32 genera, but many of them have been neglected for decades due to the lack of field collections and molecular data. In this study, we introduce a new species Fitzroyomyces hyaloseptisporus and a new combination Fitzroyomyces pandanicola. We also provide additional morphological and molecular data for Ostropomyces pruinosellus and O. thailandicus based on new collections isolated from an unidentified woody dicotyledonous host in Chiang Rai, Thailand. Taxonomic conclusions are made with the aid of morphological evidence and phylogenetic analysis of combined LSU, ITS and mtSSU sequence data. Characteristics such as the shape and septation of ascospores and conidia as well as lifestyles among genera of Stictidaceae are discussed.
... µm vs. 8-11.5 × 2-3 µm) [78]. Sphaeropezia shangrilaensis is only known from China while S. capreae and S. leucocheila were recorded from Sweden and New Zealand, respectively [18,78]. ...
... × 2-3 µm) [78]. Sphaeropezia shangrilaensis is only known from China while S. capreae and S. leucocheila were recorded from Sweden and New Zealand, respectively [18,78]. ...
Ostropales sensu lato is a large group comprising both lichenized and non-lichenized fungi, with several lineages expressing optional lichenization where individuals of the same fungal species exhibit either saprotrophic or lichenized lifestyles depending on the substrate (bark or wood). Greatly variable phenotypic characteristics and large-scale phylogenies have led to frequent changes in the taxonomic circumscription of this order. Ostropales sensu lato is currently split into Graphidales, Gyalectales, Odontotrematales, Ostropales sensu stricto, and Thelenellales. Ostropales sensu stricto is now confined to the family Stictidaceae, which includes a large number of species that are poorly known, since they usually have small fruiting bodies that are rarely collected, and thus, their taxonomy remains partly unresolved. Here, we introduce a new genus Ostropomyces to accommodate a novel lineage related to Ostropa, which is composed of two new species, as well as a new species of Sphaeropezia, S. shangrilaensis. Maximum likelihood and Bayesian inference analyses of mitochondrial small subunit spacers (mtSSU), large subunit nuclear rDNA (LSU), and internal transcribed spacers (ITS) sequence data, together with phenotypic data documented by detailed morphological and anatomical analyses, support the taxonomic affinity of the new taxa in Stictidaceae. Ancestral character state analysis did not resolve the ancestral nutritional status of Stictidaceae with confidence using Bayes traits, but a saprotrophic ancestor was indicated as most likely in a Bayesian binary Markov Chain Monte Carlo sampling (MCMC) approach. Frequent switching in nutritional modes between lineages suggests that lifestyle transition played an important role in the evolution of this family.
