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RAxML tree based on a combined LSU, TEF1α and RPB2 sequence dataset. Bootstrap support values for maximum likelihood (ML, green), maximum parsimony (MP, red) greater than 50% and Bayesian posterior probabilities (BYPP, blue) greater than 0.95 are given above and below the nodes respectively. Branches with more than 75% bootstrap for both methods (ML and MP) are thickened. The tree is rooted to Dothidea insculpta (CBS 189.58).
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Roussoella and Roussoellopsis species are mostly known from monocotyledons (large grasses, bamboo and palms). Detailed phylogenies for this group are lacking and thus their family placement and relationships with other genera are unclear. Fresh collections of several Roussoella-like species, including the type species Roussoella nitidula were made...
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... maximum likelihood analysis was performed at the CIPRES webportal (Miller et al. 2010) using RAxML v. 7.2.8 as part of the "RAxML-HPC2 on TG" tool (Stamatakis 2006). A general time reversible model (GTR) was applied with a discrete gamma distribution and four rate classes. Fifty thorough maximum likelihood (ML) tree searches were done in RAxML v. 7.2.7 under the same model, with each one starting from a separate randomised tree and the best scoring tree selected with a final ln value of -13974.356237. One thousand non parametric bootstrap iterations were run with the GTR model and a discrete gamma distribution. The resulting replicates were plotted on to the best scoring tree obtained previously. The phylogram with bootstrap values above the branches is presented in FIG. 1 by using graphical options available in TreeView (Page ...
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... Neoroussoella is described herein as a monotypic genus represented by N. bambusae, collected on dead bamboo culms in Thailand. This taxon is characterized by its semi-immersed to immersed solitary ascostromata, cylindrical asci and relatively small brown, longitudinally ribbed two-celled ascospores. The asexual morph (formed in culture) has small, hyaline conidia with smooth walls. The genus forms a basal monotypic clade in the family Roussoellaceae (FIG. 1). Saprobic on decaying bamboo culms. Ascostromata immersed under a clypeus or epidermis raised, visible as black, dome-shaped on host surface, solitary to gregarious, centrally ostiolate, multiloculate, cells of ascostromata of brown-walled textura angularis. Peridium composed of 2-3 layers of cells of textura angularis, light brown to brown thin-walled, flattened at the base. Hamathecium comprising numerous, anastomosing, hyphae-like, cellular pseudoparaphyses, branching at the apex, often constricted at the septum, and embedded in a gelatinous matrix. Asci 8-spored, bitunicate, fissitunicate, clavate, short to long pedicellate with shallow ocular chamber. Ascospores uni-biseriate, fusiform, thick-walled, 2-celled, constricted at the septum, hyaline, pale brown or yellowish brown. Asexual state Melanconiopsis or "Neomelanconium"-like. Conidiomata pycnothyrial, superficial to semi-immersed, subglobose, dark-brown to black, unilocular or multilocular, if unilocular, locules separated by vertical columns of lightly pigmented pseudoparenchyma; Peridium comprising several brown to dark brown layers with cells of textura angularis. Conidiophores reduced to annellidic conidiogenous cells, hyaline, cylindrical, smooth, formed from cells lining the inner most layer of the pycnidium. Conidia almost globose, black, aseptate, thick-walled surrounded by an entire gelatinous ...
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... on decaying bamboo culms. Ascostromata 0.3-0.8 mm wide, 1.4-2.5 mm long., immersed under a clypeus, raised, visible as black, dome-shape areas on host surface. Locules 220-260 μm high, 560-630 μm diam, depressed globose with a flattened base, single to grouped, with a central ostiole. Beak short papillate, 60-90 μm high, 60-100 μm wide. Peridium 12.5-25 μm thick at sides, composed of small compressed cells, surrounded by wedge-shaped stromatic region. Hamathecium comprising 1-2 μm wide, numerous, anastomosing, cellular pseudoparaphyses, branching at the apex, rough-walled, and embedded in a gelatinous matrix. Asci 154-190 × 21.5-26 μm, 8-spored, bitunicate, clavate, short-stalked ( Notes: The asexual state strongly suggests that it is a species of Roussoellopsis, and the molecular analyses also showed that this strain (NBRC 106246) clusterred into the Roussoella/Roussoellopsis section (FIG. 1). This fungus is characterized by its unique ovoid conidia, while other species in this genus have almost globose conidia, it appears to be new. Possibly it maybe a same species as Ro. japonica, but the asexual morph of the latter has not been yet observed, the molecular data for Ro. japonica is also not ...
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... combined LSU, TEF1α and RPB2 data set comprises 73 taxa with Dothidea insculpta as the outgroup taxon. The dataset comprises 3,101 characters after alignment, 1,750 characters were constant, and 1,062 characters were parsimony informative, while 289 variable characters are parsimony-uninformative. Bayesian, RAxML and Maximum parsimony (MP) analysis of the combined dataset resulted in phylogenetic reconstructions with largely similar topologies, and the best scoring RAxML tree is shown in FIG. 1. The most parsimony tree resulted from ITS sequences of 12 Roussoella- like taxa is shown in FIG. 2. Most of the core families of Pleosporales (Hyde et al. 2013) are included in our phylogenetic analysis (FIG. 1), and two suborders Massarineae and Pleosporineae are represented with well-supported clades. Our eight strains of Neoroussoella, Roussoella and seven referenced sequences of Roussoella and Roussoellopsis from previous studies , Tanaka et al. 2009, Phookamsak et al. 2014 in press) formed a well-supported clade (100% BS/1.00 PP) named Roussoellaceae. They show a close relationship with Versicolorisporium triseptatum and Biatriosporaceae; the latter family was introduced for the monotypic genus Biatriospora (Hyde et al. 2013). The recently introduced bambusicolous fungal families Bambusicolaceae and Tetraplosphaeriaceae (Tanaka et al. 2009, (ML, green), maximum parsimony (MP, red) greater than 50% and Bayesian posterior probabilities (BYPP, blue) greater than 0.95 are given above and below the nodes respectively. Branches with more than 75% bootstrap for both methods (ML and MP) are thickened. The tree is rooted to Dothidea insculpta (CBS 189.58). Hyde et al. 2013) were also included in the phylogenetic analysis to understand the phylogenetic relationships between these bambusicolous fungi; however the phylogenetic reconstructions based on the combined datasets analysis showed that Bambusicolaceae clustered in the suborder Massarineae and Tetraplosphaeriaceae appeared as a basal lineage of the main families of Pleosporales, and did not show a close relationship with ...
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... combined LSU, TEF1α and RPB2 data set comprises 73 taxa with Dothidea insculpta as the outgroup taxon. The dataset comprises 3,101 characters after alignment, 1,750 characters were constant, and 1,062 characters were parsimony informative, while 289 variable characters are parsimony-uninformative. Bayesian, RAxML and Maximum parsimony (MP) analysis of the combined dataset resulted in phylogenetic reconstructions with largely similar topologies, and the best scoring RAxML tree is shown in FIG. 1. The most parsimony tree resulted from ITS sequences of 12 Roussoella- like taxa is shown in FIG. 2. Most of the core families of Pleosporales (Hyde et al. 2013) are included in our phylogenetic analysis (FIG. 1), and two suborders Massarineae and Pleosporineae are represented with well-supported clades. Our eight strains of Neoroussoella, Roussoella and seven referenced sequences of Roussoella and Roussoellopsis from previous studies , Tanaka et al. 2009, Phookamsak et al. 2014 in press) formed a well-supported clade (100% BS/1.00 PP) named Roussoellaceae. They show a close relationship with Versicolorisporium triseptatum and Biatriosporaceae; the latter family was introduced for the monotypic genus Biatriospora (Hyde et al. 2013). The recently introduced bambusicolous fungal families Bambusicolaceae and Tetraplosphaeriaceae (Tanaka et al. 2009, (ML, green), maximum parsimony (MP, red) greater than 50% and Bayesian posterior probabilities (BYPP, blue) greater than 0.95 are given above and below the nodes respectively. Branches with more than 75% bootstrap for both methods (ML and MP) are thickened. The tree is rooted to Dothidea insculpta (CBS 189.58). Hyde et al. 2013) were also included in the phylogenetic analysis to understand the phylogenetic relationships between these bambusicolous fungi; however the phylogenetic reconstructions based on the combined datasets analysis showed that Bambusicolaceae clustered in the suborder Massarineae and Tetraplosphaeriaceae appeared as a basal lineage of the main families of Pleosporales, and did not show a close relationship with ...
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... 8-11.5 × 3-4 μm ( x = 9.5 × 3.5 μm, n = 30), uni-seriate or overlapping, ellipsoidal to fusiform, 2-celled, constricted at the septum, pale brown to brown, rough-walled, longitudinally ribbed. Ascospores germinated within 12 hours, initially from one cell, hyaline hyphae. Asexual morph produced on bamboo pieces on WA cultures after 2 months. Conidiomata 178-334 μm high, 180-300 μm diam, superficial, pycnidium, globose to irregular shape, flatten at the basal of pycnidium, scattered or gregarious, clustered to solitary, non ostiolate. Pycnidial wall 5-15 μm wide, composed of 4-5 layers of cells of textura angularis, 2-3 outer layers, thin-walled, dark brown to black, 1-2 inner layers, thin-walled, hyaline. Conidiophores arising from the basal cavity around conidiomata, unbranched, aseptate. Notes: Roussoella chiangraina shares superficially similar morphology with R. neopustulans and R. siamensis; however R. chiangraina has slightly smaller ascospores than the latter two species (8-11 × 3-4 μm vs. 10-12.5 × 3.5-5 μm). All phylogenetic reconstructions strongly suggested this species is phylogenetically distinct from R. neopustulans and R. siamensis (FIGS. 1, 2). In addition, this fungus formed a "Cytoplea"-like asexual morph in culture; it has much smaller conidia (3.5-4.5 × 2-2.5 μm vs. 7-10(-13) × 4-5(-6) μm) compared with Cytoplea hysterioides (asexual morph of R. hysterioides) and smooth walls, which is usually with minutely warty in C. hysterioides. The asexual morphs are not known for R. neopustulans and R. siamensis. subglobose to lenticularis with a flattened base, solitary to clustered, black, centrally ostiolate. Beak short papillate, 75-95 μm high, 60-80 μm wide. Peridium 17-25 μm thick at sides, composed of polygonal flattened cells (5-12.5 × 1.5-5 μm), surrounded by wedge-shaped stromatic region (350-600 μm wide at sides) composed of rectangular to subglobose cells (5-20 × 2-15 μm). Hamathecium comprising 1-2 μm wide, numerous, anastomosing cellular pseudoparaphyses, branching, rough-walled, and embedded in a gelatinous matrix. Asci (86-)92-117(-130) × 6-8 μm Notes: Roussoella intermedia is similar to R. pustulans, but differ in having larger asci and ascospores (asci 92-117 × 6-8 vs. 68-83 × 6.5-8.5 μm; ascospores 12-18 × 4-5 vs. 10-16 ×4-5 μm). The combined sequences phylogenetic tree (FIG. 1) indicates that these two species could be probably the same fungus. However, the ITS sequences analysis confirms that they are phylogenetically distinct species (FIG. 2). Index Fungorum: IF550663 Etymology. Named after the country from where this fungus was collected, ...
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... was placed in Didymosphaeriaceae by Lumbsch and Huhndorf (2010). However, recent molecular data did not support this and Roussoella clustered together with Arthopyreniaceae ( Schoch et al. 2009a, Tanaka et al. 2009, Zhang et al. 2012). Most taxa in Arthopyreniaceae have hemispherical ascostromata containing cellular pseudoparaphyses, fissitunicate asci and mostly hyaline ascospores ( Eriksson 1981, Cannon andKirk 2007) and these taxa are clearly unrelated. The two strains of Arthopyrenia salicis, which were included in the phylogenetic analysis ( Schoch et al. 2009a, Tanaka et al. 2009, Zhang et al. 2012) may be wrongly named or may differ from the type (Arthopyrenia cerasi). We also included these two strains (Arthopyrenia salicis) in our analysis (not in the final analysis) and they clustered in the Roussoella/Roussoellopsis clade, as in Schoch et al. (2009a), Tanaka et al. (2009) and Zhang et al. (2012). Further studies may be warranted, with the newly added data from the fresh collections and isolations, a new family Roussoellaceae is introduced to accommodate this group. Roussoellopsis could not be differentiated from Roussoella in our phylogenetic analyses, as Ro. tosaensis, Ro. macrospora and Roussoellopsis sp (NBRC 106246) grouped together with Roussoella. However, the genera are morphologically distinct. Roussoellopsis has clavate asci and large fusiform ascospores which are strongly constricted at the septum and Melanconiopsis or "Neomelanconium"-like asexual morphs, while Roussoella has cylindrical asci with Cytoplea asexual morphs. This indicates that Roussoellopsis is a distinct genus, even though the present molecular analysis does not support this. Tanaka et al. (2009) also suggested that Roussoellopsis might be a synonym of Roussoella based on the topology of the phylogenetic analysis, but stated that careful consideration must be given to the treatment. This topology may result from too few taxa in the tree. Therefore, we refrain from synonymizing the morphologically distinct Roussoellopsis whose three species share similar morphological characters under Roussoella pending further study with a larger dataset and also the lack of sequences data of the type Ro. japonica. In addition, Roussoellopsis species were considered as Didymosphaeria by Hino and Katumoto (1965). Recently, the genus Didymosphaeria has been reexamined by Ariyawansa et al. (2014, in press) based on type material and fresh collections of the type species D. epidermidis. Their results showed that Didymosphaeria clustered into the family Montagnulaceae. We also included core genera of Montagnulaceae in our phylogenetic analysis (FIG. 1), and confirm that Roussoellopsis and Didymosphaeria are distinct genera and distally ...
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... Roussoella siamensis is most similar to R. neopustulans, and it is hard to separate them based on morphological characters. However they are phylogenetically distinct from each other in all the phylogenetic reconstructions. Therefore, we describe this fungus as a new species. Saprobic on decaying bamboo culms. Ascostromata 1.3-4.8 mm diam., semi-immersed or immersed under a clypeus, raised, visible as black, dome-shape areas on host surface, scattered to gregarious. Locules 280-320 μm high, 400-430 μm diam., depressed globose with a flattened base, 2-3 grouped, ostiolate. Beak short papillate, 50-75 μm high, 55-75 μm wide. Peridium 12.5-17.5 μm thick at sides, composed of 4-6 layers of rectangular flattened cells (3.5-18 × 1.5-4.5 μm), surrounded by wedge-shaped stromatic region (210-240 μm wide at sides) composed of polygonal to subglobose cells (3.5-18 × 4-15 μm). Hamathecium comprising 1-2 μm wide, numerous, anastomosing, cellular pseudoparaphyses, branching, rough-walled, and embedded in a gelatinous matrix. Asci 135-160 × 8-10 μm ( x = 150 × 9 μm, n = 18), 8-spored, bitunicate, cylindrical, short-stalked (12-20 μm long). Ascospores 18-24.5 × 5.5-7 μm ( x = 21.5 × 6.2 μm, n = 50), uniseriate, fusiform to ellipsoidal, 2-celled, constricted at the septum, brown, verrucose wall, covered with irregular longitudinal short striations and surrounded by an entire sheath of 1-5 μm wide. Notes: Roussoella verrucispora is similar to R. hysterioides, but the asci and ascospores are considerably smaller (asci 135-160 × 8-10 μm vs. 140-210 × 8-11 μm; ascospores 18-24.5 × 5.5-7 μm vs. 18-34 × 6-8 μm) than those of R. hysterioides (Hyde et al. 1996). In addition, the ornamentation of ascospores in R. verrucispora is verrucose, while those of R. hysterioides are linear and full length. This species was named as "R. hysterioides" (HHUF 26988) in Tanaka et al. (2009). Roussoella verrucispora is also morphologically similar to R. japanensis, but differs in having slightly larger asci and ascospores (asci 135-160 × 8-10 μm vs. 107-132 × 8-9.5 μm; ascospores 18-24.5 × 5.5-7 μm vs. 16-22 × 5.5-7 μm). The ascospores of R. verrucispora have verrucose ornamentation, while those of R. japanensis are striate. Phylogenetic analysis also distinguished these species (FIGS. 1, 2). Saprobic on decaying bamboo culms. Ascostromata immersed under a clypeus or epidermis, raised, visible as black dome-shaped or flattened ovoid areas on host surface, coriaceous, solitary to gregarious, black, centrally ostiolate. Peridium composed of 2-3 layers of light brown to brown, thin-walled cells of textura angularis, with flattened base. Hamathecium comprising numerous, anastomosing, cellular pseudoparaphyses, branching at the apex, often constricted at the septum, embedded in a gelatinous matrix. Asci 8-spored, bitunicate, cylindrical, short to long pedicellate, apically rounded with an ocular chamber. Ascospores uni-biseriate or overlapping, ellipsoidal to fusiform, 2-celled, constricted at the septum, pale brown to brown or yellowish brown, rough-walled, longitudinally ribbed, surrounded by mucilaginous sheath. Asexual morphs produced on bamboo pieces on WA cultures after 2 months. Conidiomata superficial to immersed, pycnidium, uni-multiloculate, globose to subglobose or irregular shape, scattered or clustered, solitary or gregarious, indistinctly ostiolate. Pycnidial wall composed of several layers of cells of textura angularis. Conidiophores arising from the basal cavity around conidiomata, unbranched, aseptate. Conidiogenous cells annellidic, unbranched, ampulliform or lageniform or cylindrical or cylindric-clavate, hyaline, aseptate, smooth-walled. Conidia oblong to ellipsoidal, hyaline, aseptate with two guttules, ...
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... This species is similar to R. pustulans described by Ju et al. (1996) and Hyde (1997), but the ascospores are slightly narrower. The asexual morph formed in culture differs from Cytoplea in having smooth-walled, conidia and is similar to the asexual morph of R. chiangraina. However, these taxa are phylogenetically distinct (FIGS. 1, 2). Notes: Roussoella scabrispora has distinctive ascospores with a reticulate wall ornamentation. There are only two species in Roussoella that have reticulate spore wall ornamentation, namely R. angustispora and R. scabrispora, with R. angustispora having conspicuously narrower ascospores (24−28 × 6−8 vs. 26.5−35 × 10−12.5 μm) ( Zhou et al. 2003). The ascospores of this collection are slightly smaller (25−29(−32) × (7−)9−10.5 μm vs. 26.5−35 × 10−12.5 μm) than in the protologue (Hyde 1997). Roussoella scabrispora was phylogenetically closely related with Roussoellopsis species (FIG. 1). This could possibly be a species of Roussoellopsis, however, it did not produce an asexual state in culture and asci are typical of Roussoella. visible as raised, shield-shaped or dome-shaped on host surface, uni to multi locules. Locules 65-120 μm high, 127.5- 345 μm diam, lenticularis or shield-shaped with a flattened base, coriaceous, solitary to clustered, gregarious, brown to dark brown, centrally ostiolate. Peridium7-13 μm wide, composed of several layers of cells of textura angularis, thick-walled, brown to dark brown. Hamathecium comprising 1.5-2 μm wide, numerous, anastomosing, broadly cellular pseudoparaphyses, branching at the apex, smooth-walled, non-constrict at the septum, and embedded in a gelatinous matrix. Asci (65-)73-85(-94) × (6-)7-8μm ( x = 78 × 7.5 μm, n = 25), 8-spored, bitunicate, cylindrical, short to long foot-like pedicellate, apically rounded with indistinct ocular chamber. Ascospores (8-)10-12(-14) × 4-5μm ( x = 11 × 4.5 μm, n = 30), uni-seriate or overlapping, ellipsoidal to fusiform, 2-celled, constricted at the septum, brown to dark brown, rough-walled, longitudinally ribbed. Ascospores germinated within 12 hours, initially from one cell, hyaline hyphae. Asexual morph did not form in the ...
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... This species is similar to R. pustulans described by Ju et al. (1996) and Hyde (1997), but the ascospores are slightly narrower. The asexual morph formed in culture differs from Cytoplea in having smooth-walled, conidia and is similar to the asexual morph of R. chiangraina. However, these taxa are phylogenetically distinct (FIGS. 1, 2). Notes: Roussoella scabrispora has distinctive ascospores with a reticulate wall ornamentation. There are only two species in Roussoella that have reticulate spore wall ornamentation, namely R. angustispora and R. scabrispora, with R. angustispora having conspicuously narrower ascospores (24−28 × 6−8 vs. 26.5−35 × 10−12.5 μm) ( Zhou et al. 2003). The ascospores of this collection are slightly smaller (25−29(−32) × (7−)9−10.5 μm vs. 26.5−35 × 10−12.5 μm) than in the protologue (Hyde 1997). Roussoella scabrispora was phylogenetically closely related with Roussoellopsis species (FIG. 1). This could possibly be a species of Roussoellopsis, however, it did not produce an asexual state in culture and asci are typical of Roussoella. visible as raised, shield-shaped or dome-shaped on host surface, uni to multi locules. Locules 65-120 μm high, 127.5- 345 μm diam, lenticularis or shield-shaped with a flattened base, coriaceous, solitary to clustered, gregarious, brown to dark brown, centrally ostiolate. Peridium7-13 μm wide, composed of several layers of cells of textura angularis, thick-walled, brown to dark brown. Hamathecium comprising 1.5-2 μm wide, numerous, anastomosing, broadly cellular pseudoparaphyses, branching at the apex, smooth-walled, non-constrict at the septum, and embedded in a gelatinous matrix. Asci (65-)73-85(-94) × (6-)7-8μm ( x = 78 × 7.5 μm, n = 25), 8-spored, bitunicate, cylindrical, short to long foot-like pedicellate, apically rounded with indistinct ocular chamber. Ascospores (8-)10-12(-14) × 4-5μm ( x = 11 × 4.5 μm, n = 30), uni-seriate or overlapping, ellipsoidal to fusiform, 2-celled, constricted at the septum, brown to dark brown, rough-walled, longitudinally ribbed. Ascospores germinated within 12 hours, initially from one cell, hyaline hyphae. Asexual morph did not form in the ...
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A new species of Neoroussoella (Roussoellaceae) collected in Taiwan is introduced from Pennisetum purpureum with both asexual and sexual morphs. Phylogenetic analysis of a concatenated LSU-ITS-SSU-TEF1-α-RPB2 sequence dataset reveals that the new species forms a distinct sister clade with N. bambusae with high statistical support (100% ML/ 1.00 BYP...
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... Species of this genus are characterized by papillate ascomata bearing fissitunicate, cylindrical asci frequently narrower near the base, with a short furcate pedicel and filamentous, pale brown, multiseptate ascospores without swollen cells or separating into part spores (Spegazzini 1909, Thambugala et al. 2017, Tennakoon et al. 2020. Most species of this genus are found as pathogens or saprobes worldwide on Poaceae and Cyperaceae (Phookamsak et al. 2014). Twelve Ophiosphaerella species are listed in Index Fungorum (2023), eight species with molecular data. ...
... Known hosts and distribution -On Agrostis palustris in the USA (Câmara et al. 2000); on dead culms of grass in Thailand (Phookamsak et al. 2014); on stems of grass (Poaceae) in Thailand (Thambugala et al. 2017); on dead leaves of Oryza sativa in Thailand (this study). ...
... and fasciculate, spiral, scolecosporous, filiform or filamentous, not constricted at the septum, multiseptate, ascospores. The size of asci (115-145 × 9-15 μm vs. 100-135 × 7-9 μm) and ascospores (110-140 × 4−6 μm vs. 98-120 × 2-2.5 μm) is also similar to O. agrostidis (MFLUCC 11-0152) (Phookamsak et al. 2014). The nucleotide comparisons showed that our strain (MFLUCC 23-0130) is not significantly different from the strain of O. agrostidis in ITS, LSU, SSU, and tef1-α. ...
Monocotyledons are one of the important groups of flowering plants that include
approximately 60,000 species with economically important crops including coconut (Cocos nuciferanucifera), pineapple (Ananas comosus comosus), and rice (Oryza sativa sativa). Studies on these hosts are mainly focused on pathogenic fungi; only a f ew saprobic species have been reported. This study investigated the saprobic ascomycetes associated with coconut, pineapple, and rice in southern China and northern Thailand. Approximately 200 specimens were collected, and 100 fungal strains were isolated and identified to 77 species based on phylogenetic approaches and morphological characteristics. Among the 77 species, 29, 38, and 12 were found on coconut, pineapple, and rice, respectively, distributed in Dothideomycetes (41), Eurotiomycetes (one), and S ordariomycetes (35). Pseudomycoleptodiscus , Pseudosaprodesmium Pseudosetoseptoria, Pseudostriatosphaeria and
Pseudoteichospora are introduced as new genera and Anthostomella cocois, Apiospora ananas, Chromolaenicola ananasi, Epicoccum yunnanensis, Exserohi lum ananas, Hypoxylon cocois, Lasiodiplodia ananasi, Muyocopron chiangraiense, Myrmecridium yunnanense, Occultitheca ananasi, Periconia chiangraiensis, Placidiopsis ananasi, Pseudomycoleptodiscus ananas, Pseudosaprodesmium cocois, Pseudosetoseptoria oryzae, Pseudostriatosphaeria chiangraiensis,
Pseudoteichospora thailandensis, Savoryella chiangraiensis, Savoryella cocois, and Tetraploa oryzae are introduced as novel species. In addition, 51 species are reported as new hosts or geographical records, and six species are reported as new collections. Pseudopithomyces pandanicola and P. palmicola are synonymized under P. chartarum, P. diversisporus synonymized under P. atro olivaceus based on phylogenetic analyses and morphological characteristics.
Moreover, comprehensive checklists of fungi associated with coconut, pineapple, and rice are also provided.
... as the type species, which was collected from bamboo in Malaysia. This family is characterised as having semi-immersed to immersed, solitary or gregarious, clypeate ascostromata containing trabeculate pseudoparaphyses embedded in a gel matrix, long cylindrical to clavate bitunicate asci with or without obvious fissitunicate dehiscence and brown, 2-celled ornamented ascospores (Liu et al. 2014). In this study, we introduce three new records of Roussoella species, based on morpho-anatomical and molecular analyses (Fig. 2). ...
... The sequence of our Roussoella neopustulans (2022HX25) forms a well-supported clade (85% ML, 0.92 BYPP; Fig. 2) with R. neopustulan . Roussoella neopustulans was originally introduced by Liu et al. (2014), with a description of the sexual morph only. Dai et al. (2017) provided a comprehensive description and illustrations for both the sexual and asexual morphs of this species. ...
Pleosporales comprise a diverse group of fungi with a global distribution and significant ecological importance. A survey on Pleosporales (in Didymosphaeriaceae, Roussoellaceae and Nigrogranaceae) in Guizhou Province, China, was conducted. Specimens were identified, based on morphological characteristics and phylogenetic analyses using a dataset composed of ITS, LSU, SSU, tef 1 and rpb 2 loci. Maximum Likelihood (ML) and Bayesian analyses were performed. As a result, three new species ( Neokalmusia karka , Nigrograna schinifolium and N. trachycarpus ) have been discovered, along with two new records for China ( Roussoella neopustulans and R. doimaesalongensis ) and a known species ( Roussoella pseudohysterioides ). Morphologically similar species and phylogenetically close taxa are compared and discussed. This study provides detailed information and descriptions of all newly-identified taxa.
... 712 Neoroussoella Jian K. Liu, Phook. & K.D. Hyde, Phytotaxa 181(1): 21 (2014) Roussoellaceae was introduced by Liu et al. (2014) to accommodate Neoroussoella, Roussoella and Roussoellopsis. Neoroussoella was initially established to accommodate a single species, N. bambusae (Liu et al. 2014). ...
... & K.D. Hyde, Phytotaxa 181(1): 21 (2014) Roussoellaceae was introduced by Liu et al. (2014) to accommodate Neoroussoella, Roussoella and Roussoellopsis. Neoroussoella was initially established to accommodate a single species, N. bambusae (Liu et al. 2014). Eleven species have been accepted in this genus and all were introduced by incorporating morphological and molecular data (Dai et al. 2022). ...
... Eleven species have been accepted in this genus and all were introduced by incorporating morphological and molecular data (Dai et al. 2022). These species commonly live as saprobes associated with decaying branches, stems and pods of dicotyledonous and monocotyledonous plants (Liu et al. 2014, Jayasiri et al. 2019, Karunarathna et al. 2019, Phukhamsakda et al. 2020, Poli et al. 2020, Yuan et al. 2020. Neoroussoella solani was isolated from keratitis in human patients with keratomycosis (Mochizuki et al. 2017). ...
... 712 Neoroussoella Jian K. Liu, Phook. & K.D. Hyde, Phytotaxa 181(1): 21 (2014) Roussoellaceae was introduced by Liu et al. (2014) to accommodate Neoroussoella, Roussoella and Roussoellopsis. Neoroussoella was initially established to accommodate a single species, N. bambusae (Liu et al. 2014). ...
... & K.D. Hyde, Phytotaxa 181(1): 21 (2014) Roussoellaceae was introduced by Liu et al. (2014) to accommodate Neoroussoella, Roussoella and Roussoellopsis. Neoroussoella was initially established to accommodate a single species, N. bambusae (Liu et al. 2014). Eleven species have been accepted in this genus and all were introduced by incorporating morphological and molecular data (Dai et al. 2022). ...
... Eleven species have been accepted in this genus and all were introduced by incorporating morphological and molecular data (Dai et al. 2022). These species commonly live as saprobes associated with decaying branches, stems and pods of dicotyledonous and monocotyledonous plants (Liu et al. 2014, Jayasiri et al. 2019, Karunarathna et al. 2019, Phukhamsakda et al. 2020, Poli et al. 2020, Yuan et al. 2020. Neoroussoella solani was isolated from keratitis in human patients with keratomycosis (Mochizuki et al. 2017). ...
This is the eighth of the series Mycosphere notes wherein we provide descriptions and notes on various fungal genera. In this compilation, we introduce Neophaeosphaeriopsis (Phaeosphaeriaceae) as a new genus, and 25 new species. The new species are Acrodictys bambusae (Acrodictyaceae), Acrogenospora guizhouensis (Acrogenosporaceae), Aureobasidium xishuangbannaensis (Saccotheciaceae), Conlarium guizhouense (Conlariaceae), Dactylellina dulongensis (Orbiliaceae), Diaporthe araliae-chinensis (Diaporthaceae), Dibaeis jingdongensis (Icmadophilaceae), Dictyosporella yunnanensis (Annulatascaceae), Distoseptispora phragmiticola (Distoseptisporaceae), Fusarium camelliae (Nectriaceae), Helminthosporium lignicolum, Helminthosporium shangrilaense (Massarinaceae), Kirschsteiniothelia puerensis (Kirschsteiniotheliaceae), Melomastia septata (Pleurotremataceae), Montagnula aquilariae (Didymosphaeriaceae), Neophaeosphaeriopsis triseptatispora (Phaeosphaeriaceae), Neoroussoella chiangmaiensis (Roussoellaceae), Nigrograna heveae (Nigrogranaceae), Pestalotiopsis ficicrescens (Sporocadaceae), Pleurothecium hainanense (Pleurotheciaceae), Rhodoveronaea hainanensis (Sordariomycetidae), Roussoella chinensis (Roussoellaceae), Torula calceiformis (Torulaceae), Trichoglossum ailaoense (Geoglossaceae) and Zeloasperisporium spartii (Zeloasperisporiaceae). We provide new sequence data for 25 species and updated phylogenetic trees for 24 genera (Acrodictys, Acrogenospora, Aureobasidium, Conlarium, Dactylellina, Diaporthe, Dibaeis, Dictyosporella, Distoseptispora, Fusarium, Helminthosporium, Kirschsteiniothelia, Melomastia, Montagnula, Neophaeosphaeriopsis, Neoroussoella, Nigrograna, Pestalotiopsis, Pleurothecium, Rhodoveronaea, Roussoella, Torula, Trichoglossum, Zeloasperisporium).
... laevigatus resembles previous work describing the high abundance of these fungi in the soil and in association with plants, particularly in the cases of saprophagous Roussoella sp. (Liu et al. 2014) or the putative facultative phytopathogens Sclerotiniaceae sp. (Adams and Ayers 1979). ...
Prosopis laevigata (mesquite; Fabaceae) forms fertility islands in soils of semi-arid lands where microbial diversity concentrates in response to the accumulation of resources in the soil beneath individual plants, promoting organic matter decomposition and nutrient cycling. This phenomenon provides suitable conditions for the proliferation of key edaphic elements such as fungi and mites. Mite-fungal interactions are central for our understanding of nutrient cycling processes in resource-limited arid food webs; yet, no information is available about fertility islands in semi-arid lands. Thus, we aimed to determine in vitro fungal-based feeding preferences and molecular gut content of the oribatid mite species Zygoribatula cf. floridana and Scheloribates cf. laevigatus, which are abundant under the canopy of P. laevigata in an intertropical semi-arid zone in Central Mexico. Our results on the gut content analysis of these oribatid species resulted in the ITS-based identification of the following fungi: Aspergillus homomorphus, Beauveria bassiana, Filobasidium sp., Mortierella sp., Roussoella sp., Saccharomyces cerevisiae, Sclerotiniaceae sp. and Triparticalcar sp. Furthermore, under laboratory conditions both oribatid mite species exhibited feeding preferences on melanized fungi, such as Cladosporium spp., whereas A. homomorphus and Fusarium penzigi were avoided. Our findings indicated that the analyzed oribatid mite species have similar feeding preferences for melanized fungi, which might suggest resource partitioning and a degree of preference, explaining the coexistence of both oribatid species.
... Phaeosphaeriaceae is one of the species-rich families in Pleosporales and includes species that inhabit a wide range of ecosystems (Phookamsak et al. 2014, Tennakoon et al. 2020). This family was introduced by Barr (2002) which is characterized by immersed to superficial, globose to subglobose ascomata, short papilla, bitunicate asci and hyaline, yellowish or brown, fusiform to ellipsoidal, filiform, or muriform, septate ascospores. ...
... Phaeosphaeria was introduced by Miyake (1909) to accommodate P. oryzae as the type species. Phaeosphaeria species seem to have cosmopolitan in distribution since they have been recorded from both temperate and tropical countries (i.e., China, Germany, Italy, Japan, Taiwan, Thailand, USA) (Hyde et al. 2013, Hongsanan et al. 2020a, Phookamsak et al. 2014, Tennakoon et al. 2020). There are 219 epithets for Phaeosphaeria in Index Fungorum (2021). ...
... This family was introduced by Liu et al. (2014) to accommodate Roussoella with R. nitidula as the type species. Initially, three genera were accommodated in this family viz. ...
This paper elaborates the advances made in the study of morphology, phylogeny, host association and geography of novel and interesting fungi in China and Thailand. We documented saprobic microfungi from dead twigs of different plant hosts from Annonaceae (Anomianthus dulcis, Cananga odorata and Desmos chinensis), Apocynaceae (Alstonia scholaris) and Magnoliaceae (Magnolia champaca, M. garrettii and M. liliifera) in Yunnan Province, China and northern Thailand. Descriptions, illustrations and discussions on the familial placement of taxa are given based on phylogeny and morphological data. One new genus Muriformispora in Neohendersoniaceae (Dothideomycetes) and twelve new species, Acrocalymma magnoliae, Diaporthe chiangmaiensis, Fuscostagonospora magnoliae, Gyrothrix anomianthi, Hermatomyces anomianthi, Muriformispora magnoliae, Neomassaria alstoniae, N. thailandica, Neoroussoella thailandica, Peroneutypa anomianthi, Pseudochaetosphaeronema magnoliae and Torula canangae are introduced. An amended account of Hermatomyces is provided to include the sexual morph of the genus. New host records or new country records are provided for Acrocalymma pterocarpi, A. walkeri, Amphisphaeria micheliae, Angustimassarina populi, Aurantiascoma minimum, Diaporthe musigena, D. pterocarpi, Eutypella citricola, Gyrothrix oleae, Hermatomyces sphaericus, Lasiodiplodia crassispora, L. exigua, L. ponkanicola, L. pseudotheobromae, L. thailandica, L. theobromae, Magnibotryascoma kunmingense, Memnoniella ellipsoidea, Melomastia clematidis, M. thamplaensis, Neoroussoella entadae, Nectria pseudotrichia, Nigrograna thymi, Periconia byssoides, P. pseudobyssoides, Phaeosphaeria sinensis, Pseudopithomyces chartarum, Pseudofusicoccum adansoniae, Rhytidhysteron neorufulum, Setoapiospora thailandica and Xenoroussoella triseptata.
... and Roussoella Sacc. are well-documented on bamboo and palms in different localities in Asia (Liu et al. 2014;Konta et al. 2016;Dai et al. 2017). ...
... (descriptions from Samuels and Rossman 1987 Konta & Hyde) are the initial colonizers of dead palm material (Hyde 1993;Fröhlich and Hyde 1994;Hidayat et al. 2006;Konta et al. 2016). Liu et al. (2014) introduced Roussoellaceae Jian K. Liu et al. to accommodate three genera, i.e. Neoroussoella Jian K. Liu et al., Roussoella Sacc. ...
... Barr and, Pararoussoella Wanas et al., were added to this family (Hyde 1994;Hyde et al. 1996;Ariyawansa et al. 2015;Hyde et al. 2017;Phookamsak et al. 2019;Wijayawardene et al. 2020). Most species of Roussoellaceae were reported as saprophytic taxa on the terrestrial plants including bamboo, palms and mangroves (Liu et al. 2014;Jiang et al. 2019;Poli et al. 2020). The members of this family have 4-8 spored, and bitunicate asci with aseptate, brown to dark brown ascosporic, melanconiopsis-like or neomelanconium-like asexual morphs (Liu et al. 2014). ...
Several micro fungi were gathered from bamboo and palm in Guizhou Province, China. In morphology, these taxa resemble Neomassaria , Roussoella and Oxydothis . Multi-gene phylogenetic analyses based on combined ITS, LSU, SSU, rpb 2 and tef 1 loci confirmed that two are new geographical records for China, ( viz. Roussoella siamensis , Neomassaria fabacearum ), while two of them are new to science ( viz. Oxydothis fortunei sp. nov. and Roussoella bambusarum sp. nov.). The stromata of Roussoella bambusarum are similar to those of R. thailandica , but its ascospores are larger. In addition, multi-gene phylogenetic analyses show that Oxydothis fortunei is closely related to O. inaequalis , but the J- ascus subapical ring as well as the ascospores of O. inaequalis are smaller. Morphological descriptions and illustrations of all species are provided.
... The family Roussoellaceae Liu et al. [1] accommodates three genera, viz., Neoroussoella Liu et al., Roussoella Sacc. and Roussoellopsis Hino and Katum. ...
... Furthermore, there are no base pair differ-ences in ITS, tef1 and rpb2 loci of GMBCC1067, GMBCC1117, and MAFF 239636 which indicates that they are conspecific. Morphologically, they are identical to each other [1]. Hence, based on both morphology and phylogeny, we identified GMBCC1067 and GM-BCC1117 as R. japanensis. ...
... Hence, based on both morphology and phylogeny, we identified GMBCC1067 and GM-BCC1117 as R. japanensis. Roussoella japanensis was introduced by Liu et al. [1] from twigs of Sasa veitchii var. veitchii in Japan. ...
During the ongoing investigation of bambusicolous ascomycetous fungi in Yunnan, China, 24 specimens belonging to the family Roussoellaceae were collected and identified based on morphological features and phylogenetic support. Maximum-likelihood (ML) analyses and Bayesian analyses were generated based on the combined data set of ITS, LSU, tef1, and rpb2 loci. The phylogenetic analyses revealed four novel lineages in Roussoella s. str.; thus, we introduced four new species viz., Roussoella multiloculate sp. nov., R. papillate sp. nov., R. sinensis sp. nov., and R. uniloculata sp. nov. Their morphological characters were compared with the known Roussoella taxa, which lack sequence data in the GenBank. Asexual morphs of R. kunmingensis and R. padinae were recorded from dead bamboo culms in China (from the natural substrates) for the first time. Neoroussoella bambusae, Roussoella japanensis, R. nitidula, R. padinae, R. scabrispora, and R. tuberculate were also reported as the first records from China. All new taxa are described and illustrated in detail. Plates are provided for new reports.
... Roussoella is characterized by immersed clypeate ascostromata, pseudoparaphyses embedded in a gelatinous matrix, bitunicate asci, and ornamented, two-celled, brown, longitudinally striate ascospores [70,71]. Species of Roussoella can be found on bamboo and woody plants and in seawater [49,[71][72][73][74]. Currently, 39 species of Roussoella are listed in Index Fungorum (2021). ...
During a survey of plant-inhabiting fungi and water niches from Korea, noteworthy fungi were collected; among them, two new species, Paracamarosporium noviaquum sp. nov. and Phyllosticta gwangjuensis sp. nov., are described based on morphology and multi-gene phylogenies. Paracamarosporium noviaquum was characterized by its production of 1-celled and 2-celled conidia, forming conidiomata on only potato dextrose agar medium. Phyllosticta gwangjuensis was characterized by conidia hyaline, ovoid to ellipsoid shape, rounded at both ends, containing numerous guttulae or with a single large central guttule. Additional species were identified as Cosmospora lavitskiae, Monochaetia cameliae, and Roussoella doimaesalongensis, which are reported as new record species from Korea. Detailed descriptions and illustrations of these taxa are provided herein.
... Pleosporales is the largest order in the Dothideomycetes (Hyde et al. 2013, Hongsanan et al. 2020& Wijayawardene et al. 2020, including 28 families, 175 accepted genera and 12 genera listed as genera incertae sedis (Hongsanan et al. 2020). Roussoellaceae in Pleaosporales was introduced by Liu et al. (2014) and typified by Roussoella with R. nitidula. Members of ascomycetous taxa of Roussoellaceae characterized by dome-shaped to elongated linear ascostromata, with uni-loculate to multi-loculate, cylindrical to clavate, bitunicate asci, with brown, 2-celled ornamented ascospores (Liu et al. 2014). ...
... Roussoellaceae in Pleaosporales was introduced by Liu et al. (2014) and typified by Roussoella with R. nitidula. Members of ascomycetous taxa of Roussoellaceae characterized by dome-shaped to elongated linear ascostromata, with uni-loculate to multi-loculate, cylindrical to clavate, bitunicate asci, with brown, 2-celled ornamented ascospores (Liu et al. 2014). Asexual morph of the family is coelomycetous and characterized by pycnothyrial conidiomata containing globose, oblong or ellipsoidal often biguttulate, hyaline conidia (Liu et al. 2014). ...
... Members of ascomycetous taxa of Roussoellaceae characterized by dome-shaped to elongated linear ascostromata, with uni-loculate to multi-loculate, cylindrical to clavate, bitunicate asci, with brown, 2-celled ornamented ascospores (Liu et al. 2014). Asexual morph of the family is coelomycetous and characterized by pycnothyrial conidiomata containing globose, oblong or ellipsoidal often biguttulate, hyaline conidia (Liu et al. 2014). Jaklitsch & Voglmayr (2016) treated the family as a synonym of Thyridariaceae. ...
In this study, we introduce an asexual morph of a novel species Neoroussoella peltophora (Roussoellaceae) on Peltophorum vogelianum (Fabaceae) collected from Chiang Rai Province, Thailand. Morphologically the new species resembles Neoroussoella species. Phylogenetic analyses of concatenated LSU, SSU, ITS, tef1-α and rpb2 sequence data revealed that (MFLU 21–0113) is phylogenetically distinct (91% MLBS/1.00 BYPP). Neoroussoella peltophora differs from N. fulvicomae in having thinner peridium and differs from N. alishanense in having thicker peridium. Further, N. peltophora is distinct from N. alishanense in having shorter conidiogenous cells and differs from N. fulvicomae in having longer conidiogenous cells. The base pair comparison of ITS, tef1-α and rpb2 gene regions also support that our isolate is distinctive from the other species in Neoroussoella.
