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Quantitative and semi-quantitative identification of central line-associated bloodstream infections, MacConkey agar showing growth of mucoid, lactose fermenter colonies
Source publication
Background: Complications associated with central venous catheter central line central line-associated bloodstream infections (CLABSIs).
Methods: We prospective by studied the microbiological profile and antimicrobial susceptibility patterns of isolated bacteria from CLABSIs in respiratory intensive care unit at our a tertiary care teaching hospit...
Context in source publication
Context 1
... from at least two separate blood draws was collected on the same or consecutive calendar days under strict asepsis. Catheter tip samples were processed for both semi-quantitative method (roll-plate) and quantitative method (vortex) [ Figure 1]. [5] According to it, isolation of >15 colony-forming unit (CFU)/mL can be considered as significant for semiquantitative method and >100 CFU/mL for quantitative method. ...
Citations
... Acinetobacter baumanii (40%) and Pseudomonas aeruginosa (75%)which is co relating with other study Montrucchio, G et al study. 29,30 While 90% of Klebsiella pneumoniae, 75% of Escherichia coli, and 100% of Acinetobacter baumanii were ESBL producers, All gram-negative bacteria were sensitive to polymyxin Bwhich is co relating with other studyKhodare A et al. [31][32][33] Among the gram-positive cocci isolates, 66.67% of staphylococci were methicillin-resistant Staphylococcus aureus (MRSA) and 71.5% were methicillin-resistant coagulase-negative staphylococci (MR-CoNS). MRSA and MR-CoNS were both susceptible to vancomycin and linezolide; similar findings were reported in different studies. ...
... MRSA and MR-CoNS were both susceptible to vancomycin and linezolide; similar findings were reported in different studies. 33 Out of 40 cases of CLABSI, three patients died: one from renal failure, one from sepsis with multi-organ disorder syndrome (MODS), and two from chronic obstructive pulmonary disease. ...
Central venous catheters are commonly inserted to monitor patients with critical illnesses. Even when used to treat very ill patients, they are susceptible to widespread headaches, including central line-associated bloodstream infections (CLABSI). Central line-associated bloodstream infection (CLABSI) is one of the most significant HAIs, associated with excess mortality of 12–25%. To discover CLABSI cases, microbiological profiles, and their antimicrobial susceptibility. The study was conducted in an intensive care unit over a period of 12 months. 150 blood samples and catheter tips were collected for the culture of suspected or secondary bacteremia. CLABSI is described as being consistent with the CDC’s proposal. Automated VITEK 2 technology identifies bacterial isolates and investigates their antimicrobial susceptibility. Out of 150 samples, 50 showed no growth, 45 showed colonizers, 40 showed CLABSI, and 15 showed secondary infection. Fifty-five had positive blood cultures, 15 of whom had another source of infection. In our study, the CLABSI rate was 7.8/1000 central venous days. Rigorous implementation of the system and maintenance of the central line bundle are mandatory to prevent colonization.
