Fig 26 - uploaded by Vladimir E. Fedosov
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Pylaisia brotheri (B, OK2799, Russia, Primorsky Territory, Ussurijsky Reserve), perisome under light microscope, showing endostome split along the keel nearly to the top of segments and its extensive adherence to exostome teeth. Scale bars: 100 μm for A; 50 μm for B-C.
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... Because the coverage of the genera Pylaisia and Homomallium for the plastid trnL-trnF and rps4 regions is relatively low in GenBank, we also attempted to obtain sequences of the nuclear ribosomal ITS region, which is much better represented due to the treatment published by Ignatova et al. (2020). Accessions of sequences used for this study are specified in Appendix 1. ...
... In recent years, five Pylaisia species were either newly described or resurrected from previously synonymised taxa (Lin et al. 2010;Allen and Buck 2015;Ignatova et al. 2020). Arikawa (2004) recognised 15 species, including P. levieri (Müll.Hal.) ...
... In the most extensive study of the group, Ignatova et al. (2020) included 15 species of Pylaisia (out of around 20 in total) and four species of Homomallium (from around 15), albeit using mostly ITS data, with additional ETS data for a subset of accessions and the less variable trnL-trnF region on an even more limited subset. The monophyly of the genus was confirmed using the concatenated data, although species referrable to Giraldiella (with possibly two species, cf Lin et al. 2010) were not included, and neither was the monotypic Pylaisiopsis (Broth.) ...
Introduction. Trolliella euendostoma Herzog is the single species of a monospecific genus and we know of no molecular study since its description and we know of only one report since its discovery. Therefore, we decided to compare a recently collected Myanmar specimen with the type material deposited in JE and obtain molecular data from the new material with which to explore the phylogenetic affinities of this species.
Methods. In addition to a morphological examination, an analysis of ITS and trnF–trnS sequence data from the newly collected Myanmar specimen of Trolliella euendostoma was carried out. Phylogenetic affinities were assessed using Bayesian inference and maximum
likelihood analyses.
Results and discussion. Molecular affinities and morphological features of Trolliella euendostoma suggest that the species should be excluded from the Sematophyllaceae and transferred to Pylaisia Schimp. in the Pylaisiaceae. Consequently, we propose the new
combination Pylaisia euendostoma (Herzog) Frank Müll., Jan Kučera & S.He, select the lectotype for T. euendostoma, and additionally synonymise Pylaisia kunisawae (Ando) Ochyra (≡ Pylaisiella kunisawae Ando) with P. euendostoma.
... This species was described from Alaska (Ando, Higuchi, 1987). In Russia, according to Ignatova et al. (2020) it is known from the Chukotka Autonomous Area, Republic of Sakha (Yakutia), Amur Region, Khabarovsk Territory, Trans-Baikal Territory, Republic of Buryatia, Republic of Altai. But it should be noted that the record of Pylaisia steerei for the Trans-Baikal Territory in this publication is erroneous, because the localities of this species in Trans-Baikal Territory are not given in the list of studied specimens (Ignatova et al., 2020) and lacking in the Moss flora of Russia database (Ivanov et al., 2017). ...
... In Russia, according to Ignatova et al. (2020) it is known from the Chukotka Autonomous Area, Republic of Sakha (Yakutia), Amur Region, Khabarovsk Territory, Trans-Baikal Territory, Republic of Buryatia, Republic of Altai. But it should be noted that the record of Pylaisia steerei for the Trans-Baikal Territory in this publication is erroneous, because the localities of this species in Trans-Baikal Territory are not given in the list of studied specimens (Ignatova et al., 2020) and lacking in the Moss flora of Russia database (Ivanov et al., 2017). Thus, the finding of P. steerei in the Kalar District is the first reliable record for the Trans-Baikal Territory. ...
... Thus, the finding of P. steerei in the Kalar District is the first reliable record for the Trans-Baikal Territory. The nearest known localities of this species are in the Republic of Buryatia, Republic of Sakha (Yakutia), and in Xinjiang Province of China (Ignatova et al., 2020). ...
First records for Russia of naviculoid diatom from the Yaroslavl Region, and micromycetes from the Republic of North Ossetia — Alania, green alga for the Leningrad Region and Yamal-Nenets Autonomous Area, fragilarioid diatom for the Kaliningrad Region, red alga for the Nizhny Novgorod Region, cyanoprokaryota for the Leningrad Region and Chukotka Autonomous Okrug, and crustaceous red alga for the Autonomous Republic of Adjara of Georgia, macromycetes for the Leningrad Region, Khanty-Mansi Autonomous Area — Yugra, Republic of Tuva, Trans-Baikal Territory, myxomycetes for the Trans-Baikal Territory, lichens and allied fungi for the Murmansk and Tver regions, republics of Karelia and Tuva, Yamal-Nenets Autonomous Area, Altai and Khabarovsk territories, cyanolichen for the Urals and the Orenburg Region, mosses for the Lipetsk Region, republics of Ingushetia and Buryatia, Krasnoyarsk and Trans-Baikal territories are presented. The data on their localities, habitats, distribution are provided. The specimens are kept in the herbaria ALTB, GSU, IBIW, IRK, KPABG, LE, MHA, MW, NNSU, NSK, PZV, TBI, UUH, VU, YSU, and the Diatom collection of the Laboratory for Algology of IBIW RAS. Sequences of 16S, and 16S–23S ITS cyanobacterial RNA regions, ITS1-5.8S-ITS2 fungal and ITS1-2 moss nrDNA regions of some specimens have been deposited in the GenBank.
... Since the state of liverwort diversity knowledge in Russian Asia is still fragmentary, there were no similar works in other genera of hepatics in Russian Asia, and this is the first attempt to resolve such kind of problem. At the same, molecular phylogenetic studies of Asian mosses revealed several cases of such "problematic" topologies [5][6][7]. ...
This paper addresses the genetic delimitation of narrowly distributed Scapania magadanica and broadly circumpolar S. kaurinii. The phylogenetic trees based on maximum likelihood and Bayesian inference constructed for one of the most informative loci (ITS1‒2) showed that Scapania magadanica was deeply nested within the clade of S. kaurinii. The comparison of the obtained topologies with known strong morphological dissimilarities of two taxa has led to the understanding that this approach does not work. The latter may be due to a widespread variable tentatively ‘ancestral’ species (S. kaurinii) having no joint molecular synapomorphies that would delimit it from the locally distributed derived taxon (S. magadanica). Therefore, the relationships of these two species were evaluated using molecular genetic distances with the Neighbor Net split network and TCS haplotype network. The obtained data have confirmed the speculation above, and it is possible to assume that when the S. magadanica lineage split, S. kaurinii already occupied a rather wide range, which could limit further gene flow among its remote populations.
Introduction. We report the results of an international bryological expedition to the Khamar-
Daban mountain range in Russian southern Siberia. The novel approach of integrative floristics
is introduced here as a method of surveying the flora of a region by using a combination of
morphological and molecular evidence to identify and verify unexpected findings. We show
that use of this approach can deliver a high level of credibility in cases in which direct
molecular comparison is possible or the relevant data can be easily obtained from reliable
studies informing recent treatments. Integrative floristics can also be used to identify
potential taxonomic problems requiring further study.
Methods. Conventional identification methods based on plant anatomy and morphology were
supplemented with molecular barcoding (nuclear ITS, plastid trnL–trnF, rps4 and atpB–rbcL
regions) of specimens that either did not fit existing morphological concepts perfectly or for
which records were unexpected based on previously established phytogeographical patterns.
Key results and conclusions. In total, we recorded ca 380 species of mosses and ca 150
species of liverworts from five localities on the northern slopes of the Khamar-Daban range.
Such floristic richness indicates a hotspot of bryophyte diversity with a remarkably high
proportion of suboceanic species, such as Brachydontium trichodes (F.Weber) Milde,
Herzogiella striatella (Brid.) Z.Iwats. and Racomitrium macounii subsp. alpinum (E.Lawton)
Frisvoll. Nearly 20% of specimens were identified or checked using DNA barcoding. This
approach showed the presence of previously unrecorded species in a number of genera,
including Didymodon, Neckera and Pseudohygrohypnum.
We present an integrative molecular and morphological study of subaquatic representatives of the genus Pseudohygrohypnum (Pylaisiaceae, Bryophyta), supplemented by distribution modelling of the revealed phylogenetic lineages. Phylogenetic analyses of nuclear and plastid datasets combined with the assemble species by automatic partitioning (ASAP) algorithm revealed eight distinct species within the traditionally circumscribed P. eugyrium and P. subeugyrium. These species are therefore yet another example of seemingly widely distributed taxa that harbour molecularly well-differentiated lineages with narrower distribution ranges. Studied accessions that were previously assigned to P. eugyrium form three clearly allopatric lineages, associated with temperate regions of Europe, eastern North America and eastern Asia. Remarkably, accessions falling under the current morphological concept of P. subeugyrium were shown to be even more diverse, containing five phylogenetic lineages. Three of these lineages occur under harsh Asian continental climates from cool-temperate to Arctic regions, while the remaining two, referred to P. subeugyrium s.str. and P. purpurascens, have more oceanic North Atlantic and East Asian distributions. Niche identity and similarity tests suggested no similarity in the distributions of the phylogenetically related lineages but revealed the identity of two East Asian species and the similarity of two pairs of unrelated species. A morphological survey confirmed the distinctness of all eight phylogenetic lineages, requiring the description of five new species. Pseudohygrohypnum appalachianum and P. orientale are described for North American and East Asian plants of P. eugyrium s.l., while P. sibiricum, P. subarcticum and P. neglectum are described for the three continental, predominantly Asian lineages of P. subeugyrium s.l. Our results highlight the importance of nontropical Asia as a center of bryophyte diversity. Phylogenic dating suggests that the diversification of subaquatic Pseudohygrohypnum lineages appeared in late Miocene, while mesophilous species of the genus split before Miocene cooling, in climatic conditions close to those where the ancestor of Pseudohygrohypnum appeared. We speculate that radiation of the P. subeugyrium complex in temperate Asia might have been driven by progressive cooling, aridification, and increases in seasonality, temperature and humidity gradients. Our results parallel those of several integrative taxonomic studies of North Asian mosses, which have resulted in a number of newly revealed species. These include various endemics from continental areas of Asia suggesting that the so-called Rapoport’s rule of low diversity and wide distribution range in subpolar regions might not be applicable to bryophytes. Rather, the strong climatic oscillations in these regions may have served as a driving force of speciation and niche divergence.
