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Advances Polyphenol Oxidase (PPO) from Sorghum bicolor (white and yellow varieties) grains were investigated for optimum processing condition. The partially purified enzyme was obtained from two varieties of Sorghum bicolor by step-wise separation through ion-exchange and size-exclusion chromatography. The final purification gave a yield of 7.33 %...
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... polyphenol oxidase activity of white sorghum was found to be 2.234 U.mL -1 and 2.055 U.mL -1 for crude and ammonium sulphate precipitation samples respectively while that of yellow sorghum was 2.560 U.mL -1 and 5.300 U.mL -1 for crude and ammonium sulphate precipitate samples respectively (Table 1). The elution profile of both varieties showed the same pattern. ...Citations
... Sorghum grain is commonly milled to make traditional dishes like ugali, which serves as a substitute for maize. It is often mixed with other grains like cowpeas, amaranthus, and green grams to create nutritious meals (Fatoki & Sanni, 2019). Sorghum millets based nonalcoholic beverages are popular all over the world. ...
... Sorghum (Sorghum bicolor L. Moench) stands as the fifth most cultivated cereal globally and serves as a dietary staple for over 500 million people across 30 countries in semi-arid regions worldwide, including the Americas, Asia, Australia, and Africa (Dahlberg et al., 2011;Fatoki and Sanni, 2019). This gluten-free and drought-tolerant cereal grain plays a versatile role in human nutrition, being used in various forms like bread, malt drinks, beer, and even as livestock feed and a renewable energy source, notably bioethanol (Dahlberg et al., In Silico Evaluation of Nutri-Pharmacological Potentials of Phytochemicals Fatoki et al. 2011). ...
Sorghum (Sorghum bicolor L. Moench) ranks as the fifth most widely grown cereal globally, and its grain is gluten-free, containing about 26 phytochemicals which are mostly phenolics. The objective of this study was to predict pharmacokinetics of sorghum grain phytochemicals and some molecular targets that may impact good health in human. The methods used were in silico pharmacokinetic prediction, target prediction, target gene network analysis, docking and molecular dynamics simulation. The results showed that ferulic acid, p-coumaric acid, p-hydroxybenzoic acid, and vanillic acid were blood-brain barrier permeant, while p-coumaric acid and gallic acid have high gastrointestinal absorption. The results indicated that p-coumaric acid has 100% probability of target on aldose reductase and estrogen receptor, while gallic acid had 100% probability of target on alpha-(1,3)-fucosyltransferase 7. The docking analyses revealed that p-coumaric acid bind to aldose reductase with an affinity of -7.759 kcal.mol-1 while gallic acid bind to alpha-(1,3)-fucosyltransferase 7 with binding affinity of -5.512 kcal.mol-1. Overall, binding energy ΔGbind (Total) at 0 ns was slightly higher than that of 100 ns for p-coumaric acid - aldose reductase complex (-56.631 to -53.546 kcal.mol-1). This study provides valuable insights into the potential pharmacological actions of phytochemicals in sorghum grains.
... ) stability of PPO from three lily cultivars. The names of the three cultivars of lily are Lilium lancifolium Thunb (JD), Lilium brownie var. viridulum (LY) and Lilium davidii var. unicolor cotton (LZ)activity of PPO decreased remarkably, when the temperature decreased from 15 to 5°C. In most cases, the optimum temperature for plant PPO is 30-40°C.Fatoki and Sanni (2019) found that the optimum activity for white and yellow sorghum was obtained at 30 and 40°C, respectively, by using catechol as the reactant. The optimum temperature of PPO from apple and Tadela date fruit is also within this range(Aydin, Gulcin, & Alwasel, 2015;Benaceur et al., 2019). By contrast, the PPO from lily bulbs showed a low opti ...
The biochemical properties and thermal inactivation of polyphenol oxidase (PPO) from three main planted lily cultivars in China, namely, Lilium lancifolium Thunb, Lilium brownie var. viridulum, and Lilium davidii var. unicolor cotton were evaluated. Data indicate that the PPO from three cultivars showed two optimum pH levels of 4.0 and 6.5–7.0 and temperature of 15°C and exhibited the highest affinity toward 4‐methylcatechol. However, this enzyme did not exhibit monophenolase activity. Thiourea and L‐cysteine were more effective than other inhibitors. The enzymatic activity of L. lancifolium Thunb PPO crude extract was higher than that of L. brownie var. viridulum and L. davidii var. unicolor cotton. For thermal inactivation, L. davidii var. unicolor cotton PPO showed the best thermal resistance at 65–75°C, and L. lancifolium Thunb showed stability at 45°C. The deactivation of the three types of PPO followed the first‐order reaction kinetics, and the activation energy (Ea) was 144.28, 138.00, and 107.12 kJ/mol for L. lancifolium Thunb PPO, L. brownie var. viridulum PPO, and L. davidii var. unicolor cotton PPO, respectively.
Practical applications
Lilium is an ornamental and edible plant typically used for food and traditional Chinese medicine. Its flowers are used for decoration, and its underground bulbs are rich in various bioactive substances. Fresh lily bulbs easily turn brown and lose economic value during storage and processing. Polyphenol oxidase (PPO) is a crucial molecule involved in the enzymatic browning of fruit and vegetables. In this study, PPO was extracted from three main planted lily cultivars in China. Namely, Lilium lancifolium Thunb, Lilium brownie var. viridulum, Lilium davidii var. unicolor cotton and was partially characterized. The results are of considerable importance to further understand the PPO of lily bulbs and provide guidance for the inactivation of enzymes and the processing of lily bulb juice.
