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Pseudocoleophoma puerensis (ZHKU 22-0118). (a,b) Ascomata on a decayed branch of Coffea arabica; (c) section of an ascoma; (d) peridium at side; (e) pseudoparaphyses; (f-j) immature and mature asci (arrows indicate the club-like pedicel); (k-n) ascospores; (o) germinated ascospore; (p) culture on PDA from above and reverse (60 days). Scale bars: (c,e) = 100 µm; (f-j) = 20 µm; (k-n) = 5 µm; (o) = 10 µm. Pseudocoleophoma yunnanensis L. Lu and Tibpromma, sp.nov. (Figure 3). Index Fungorum number: IF 559422; Faces of Fungi number: FoF 12762.
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In Yunnan Province, the coffee-growing regions are mainly distributed in Pu’er and Xishuangbanna. During the surveys of microfungi associated with coffee in Yunnan Province, seven taxa were isolated from coffee samples. Based on molecular phylogenetic analyses of combined ITS, LSU, SSU, rpb2, and tef1-α sequence data and morphological characteristi...
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Coffee is one of the most important cash crops in Yunnan Province, China. Yunnan is ranked as the biggest producer of high-quality coffee in China. During surveys of microfungi from coffee plantations in Yunnan, six fungal strains that resemble Nigrogranaceae were collected. Multi-gene analyses of a combined SSU-LSU-ITS-rpb2-tef1-α sequence data ma...
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... However, most studies were restricted to certain groups of ascomycetes, such as bambusicolous fungi (Jiang et al. , 2021bDai et al. 2022;Phookamsak et al. 2022), cordycipitoid fungi Fan et al. 2021;Dong et al. 2022;Tang et al. 2023), endolichenic fungi (Si et al. 2021(Si et al. , 2023, lignicolous freshwater fungi (Luo et al. 2018a(Luo et al. , b, 2019Su et al. 2018;Dong et al. 2020;Shen et al. 2022), nematode-trapping fungi (Zhang et al. , b, c, 2023Yang et al. 2023b) and woody litter-inhabiting fungi Wanasinghe et al. 2022), as well as fungi associated with specific host plants (e.g. Camellia, Coffea, Magnolia, Mangifera and Rhododendron) Gu et al. 2022;Lu et al. 2022;Tibpromma et al. 2022;Wijayawardene et al. 2022a;Yang et al. 2022aYang et al. , b, 2023a. Comparable with the total estimated number of species that may be found in this region, these fungal inventories are still only representing a small number of extant ascomycetes in Yunnan. ...
This article presents the results of an ongoing inventory of Ascomycota in Yunnan, China, carried out as part of the research project series “Exploring ascomycete diversity in Yunnan”. From over 100 samples collected from diverse host substrates, microfungi have been isolated, identified and are currently being documented. The primary objective of this research is to promote the discovery of novel taxa and explore the ascomycete diversity in the region, utilising a morphology-phylogeny approach. This article represents the second series of species descriptions for the project and introduces three undocumented species found in the families Bambusicolaceae, Dictyosporiaceae and Periconiaceae, belonging to the suborder Massarineae (Pleosporales, Dothideomycetes). These novel taxa exhibit typical morphological characteristics of Bambusicola, Periconia and Trichobotrys, leading to their designation as Bambusicola hongheensis, Periconia kunmingensis and Trichobotrys sinensis. Comprehensive multigene phylogenetic analyses were conducted to validate the novelty of these species. The results revealed well-defined clades that are clearly distinct from other related species, providing robust support for their placement within their respective families. Notably, this study unveils the phylogenetic affinity of Trichobotrys within Dictyosporiaceae for the first time. Additionally, the synanamorphism for the genus Trichobotrys is also reported for the first time. Detailed descriptions, illustrations and updated phylogenies of the novel species are provided, and thus presenting a valuable resource for researchers and mycologists interested in the diversity of ascomycetes in Yunnan. By enhancing our understanding of the Ascomycota diversity in this region, this research contributes to the broader field of fungal taxonomy and their phylogenetic understanding.
... The Cauca farm exhibited a distinct abundance of Pseudocoleophoma sp. that accounted for >75% of reads within the saprotrophic group and was identified as an indicator species of this site. Pseudocoleophoma is a genus belonging to Dictyosporiaceae and was recently isolated from decayed wood of Coffea arabica plantations in China (Lu et al., 2022a). According to the authors, their finding represents the first report of coffee-associated fungi in Dictyosporiaceae, and our results support this novelty. ...
Fungi are essential players in the maintenance of global coffee productivity, but their taxonomic and functional diversity in tropical and subtropical soils of Latin America remains largely unexplored. To address this concern, soil fungi were surveyed in six farms in three traditional coffee-growing regions of Colombia (Cauca, Magdalena, and Risaralda). Five farms were organic and newly established (<1 to 15 years) with low shade, and one farm was under long-term conventional management (>30 years old) with higher shade cover. We used amplicon sequencing and functional prediction based on the FUNGuild annotation tool. Fungal community composition diverged among farms, with Mortierella (Mortierellomycota) and Saitozyma (Basidiomycota) among the most prevalent genera. Functional prediction revealed the predominance of saprotroph-symbiotroph and pathotroph fungi. The endophyte and litter decomposer Mortierella genus was dominant within the saprotrophs and symbiotrophs. The pathotroph community was characterized by insect pathogen species belonging to the Metarhizium (Ascomycota) genus. Indeed, M. anisopliae and M. marquandii were identified as indicator species in the conventional long-term shaded farm. This study revealed that coffee plantations studied sustain a diverse fungal community and nurture potentially beneficial species. Further studies are needed to elucidate how particular management practices can nourish beneficial fungi, suppress detrimental species, and promote more sustainable coffee production.
... The obtained forward and reverse sequences of the new taxa were assembled using the Geneious program (9.1.2) (Lu et al. 2022, https://www.geneious.com/). The assembled sequences were used to perform BLAST search in GenBank. ...
Macadamia integrifolia, one of the cash crops widely grown in the tropical and subtropical regions produces edible kernels with high oil content. Several fungal pathogens have been reported from this host, but very few studies on saprobic fungi have been carried out. As a part of our studies on saprobic fungi of edible nuts, dead branches of Macadamia integrifolia with fungal fruiting bodies were collected from Yunnan Province of China. Molecular analyses of combined SSU, ITS, LSU, RPB2, and TEF1-α sequence data showed that our collection belongs to Bambusicolaceae and forms a distinct branch with other genera in this family. Our collection fits well with the Bambusicolaceae, and morphology is similar to Palmiascoma but can be distinguished by molecular data. Therefore, we introduce our collection as Neopalmiascoma gen. nov., with N. macadamiae as the type species. A description, photo plate, and phylogenetic tree to show the taxonomic placement of the new species are provided.
... Morphologically, sexual morphs of Montagnula have three different types of ascospores (didymospore, phragmospore, and dictyospore) [8,16]. Phylogenetically, species with the same type of ascospore tend to cluster together [9,17]. In recent years, there have been many reports on Montagnula species [8,9,[18][19][20], but there are very few comprehensive and systematic papers. ...
... Montagnula species occur on terrestrial habitats with a wide geographic and host distribution [8,21]. Most Montagnula species have been found on dead leaves and twigs by their sexual morph [8,10,17,18,[21][22][23]. The sexual morph is characterized by globose to pyriform, immersed to erumpent or superficial, brown to dark brown ascomata with or without ostiole, textura angularis peridium. ...
... The new sequences derived from this study were deposited in GenBank. 17 ...
Four stains were isolated from two fresh twigs of Helwingia himalaica and two dead woods during investigations of micro-fungi in China and Thailand. Phylogenetic analyses of four gene regions LSU, ITS, SSU and tef1-α revealed the placement of these species in Montagnula. Based on the morphological examination and molecular data, two new species, M. aquatica and M. guiyangensis, and a known species M. donacina are described. Descriptions and illustrations of the new collections and a key to the Montagnula species are provided. Montagnula chromolaenicola, M. puerensis, M. saikhuensis, and M. thailandica are discussed and synonymized under M. donacina.
... Five partial gene regions were used in this study viz. the internal transcribed spacer (ITS) region was amplified with the primers ITS4 and ITS5 (White et al. 1990), the 18 s small subunit (SSU) region was amplified by primers NS1 and NS4 (White et al. 1990), the nuclear ribosomal 28 s large subunit (LSU) region was amplified by the primers LROR and LR5 (Vilgalys and Hester 1990), the partial RNA polymerase II subunit (rpb2) region was amplified with the primers RPB2-5F and RPB2-7cR (Liu et al. 1999), and the partial translation elongation factor 1-alpha (tef1-α) gene was amplified with the primers EF1-983F and 2218R (Rehner and Buckley 2005). Lu et al. (2022b) was followed for the amplification reactions of different primers. Amplified PCR products were sent to Sango Biotechnology Co., Ltd. ...
Coffee is one of the most important cash crops in Yunnan Province, China. Yunnan is ranked as the biggest producer of high-quality coffee in China. During surveys of microfungi from coffee plantations in Yunnan, six fungal strains that resemble Nigrogranaceae were collected. Multi-gene analyses of a combined SSU-LSU-ITS-rpb2-tef1-α sequence data matrix were used to infer the phylogenetic position of the new species in Nigrograna while morphological characteristics were used to deduce the taxonomic position of the new species. Six fungal strains isolated from decaying branches of Coffea arabica represent three new saprobic species in Nigrograna. The three new species, N. asexualis, N. coffeae, and N. puerensis, are described with full (macro and micro characteristics) descriptions, illustrations, and a phylogenetic tree that shows the phylogenetic position of new taxa.
During a survey of saprobic fungi on coffee samples in Yunnan Province, China, in 2020 and 2022, fungal fruiting bodies on decaying Liberian coffee twigs were collected and isolated. Maximum likelihood and Bayesian analyses of combined ITS, LSU, and RPB2 genes showed that the two isolated fungal strains belonged to a distinct species in Coniocessiaceae and were well separated from closely related genera with 73% ML and 0.92 PP statistical support. Morphologically, this distinct species can be differentiated from other genera in Coniocessiaceae by aseptate hamathecium, 4-8-spored asci with a flat apical ring, and fusiform ascospores, conical at the lower end. Based on morphology and phylogenetic analyses, the two fungal strains are described as a distinct new genus Pseudoconiocessia, within Coniocessiaceae. Pseudoconiocessia is introduced here with Pseudoconiocessia xishuangbannaensis as the type species.
Whilst conducting surveys of lignicolous microfungi in Yunnan Province, we collected a large number of taxa that resemble Montagnula (Didymosphaeriaceae, Pleosporales). Our phylogenetic study on Montagnula involved analysing sequence data from ribosomal RNA genes (nc18S, nc28S, ITS) and protein-coding genes ( rpb 2, tef 1-α). We present a biphasic approach (morphological and molecular phylogenetic evidence) that supports the recognition of four new species in Montagnula viz ., M. lijiangensis , M. menglaensis , M. shangrilana and M. thevetiae . The global diversity of Montagnula is also inferred from metabarcoding data and published records based on field observations. Metabarcoding data from GlobalFungi and field observations provided insights into the global diversity and distribution patterns of Montagnula . Studies conducted in Asia, Australia, Europe, and North America revealed a concentration of Montagnula species, suggesting regional variations in ecological preferences and distribution. Montagnula species were found on various substrates, with sediments yielding a high number of sequences. Poaceae emerged as a significant contributor, indicating a potential association between Montagnula species and grasses. Culture-based investigations from previously published data revealed Montagnula species associations with 105 plant genera (in 45 plant families), across 55 countries, highlighting their wide ecological range and adaptability. This study enhances our understanding of the taxonomy, distribution, and ecological preferences of Montagnula species. It emphasizes their role in the decomposition of organic matter in grasslands and savannah systems and suggests further investigation into their functional roles in ecosystem processes. The global distribution patterns and ecological interactions of Montagnula species underscore the need for continued research and conservation efforts.
Coffee is one of the most important cash crops native to north-central Africa, and the second most popular beverage ingredient worldwide. Coffee-associated fungal pathogens have been well studied, but few studies have reported saprobes of coffee. In China, Yunnan Province is the main region of coffee plantations and production. To investigate saprobic fungi of coffee plants, dead branches of Coffea with fungal fruiting bodies were collected in Baoshan City, Yunnan Province, China. Multi-gene phylogenetic analyses of SSU, ITS, LSU, RPB2, and TEF1-α sequence data indicated that our collections formed an independent branch within Brunneofusispora and sister to B. sinensis. The new species B. baoshanensis, characterized by uniloculate ascomata with an erumpent long neck, and inequilateral, ends acute, 1-septate, constricted at the septum ascospores with mucilaginous sheath, was identified based on morphology and multi-gene phylogeny. This is the first report of Brunneofusispora species isolated from Coffea in China. A complete description, micrographs, and a phylogenetic tree showing the placement of the new species are provided.
This study documents the morphology and phylogeny of ascomycetes collected from karst landscapes of Guizhou Province, China. Based on morphological characteristics in conjunction with DNA sequence data, 70 species are identified and distributed in two classes (Dothideomycetes and Sordariomycetes), 16 orders, 41 families and 60 genera. One order Planisphaeriales, four families Leptosphaerioidaceae, Neoleptosporellaceae, Planisphaeriaceae and Profundisphaeriaceae, ten genera Conicosphaeria, Karstiomyces, Leptosphaerioides, Neoceratosphaeria, Neodiaporthe, Neodictyospora, Planisphaeria, Profundisphaeria, Stellatus and Truncatascus, and 34 species (Amphisphaeria karsti, Anteaglonium hydei, Atractospora terrestris, Conicosphaeria vaginatispora, Corylicola hydei, Diaporthe cylindriformispora, Dictyosporium karsti, Hysterobrevium karsti, Karstiomyces guizhouensis, Leptosphaerioides guizhouensis, Lophiotrema karsti, Murispora hydei, Muyocopron karsti, Neoaquastroma guizhouense, Neoceratosphaeria karsti, Neodiaporthe reniformispora, Neodictyospora karsti, Neoheleiosa guizhouensis, Neoleptosporella fusiformispora, Neoophiobolus filiformisporum, Ophioceras guizhouensis, Ophiosphaerella karsti, Paraeutypella longiasca, Paraeutypella karsti, Patellaria guizhouensis, Planisphaeria karsti, Planisphaeria reniformispora, Poaceascoma herbaceum, Profundisphaeria fusiformispora, Pseudocoleophoma guizhouensis, Pseudopolyplosphaeria guizhouensis, Stellatus guizhouensis, Sulcatispora karsti and Truncatascus microsporus) are introduced as new to science. Moreover, 13 new geographical records for China are also reported, which are Acrocalymma medicaginis, Annulohypoxylon thailandicum, Astrosphaeriella bambusae, Diaporthe novem, Hypoxylon rubiginosum, Ophiosphaerella agrostidis, Ophiosphaerella chiangraiensis, Patellaria atrata, Polyplosphaeria fusca, Psiloglonium macrosporum, Sarimanas shirakamiense, Thyridaria broussonetiae and Tremateia chromolaenae. Additionally, the family Eriomycetaceae was resurrected as a non-lichenized family and accommodated within Monoblastiales. Detailed descriptions and illustrations of all these taxa are provided.
