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Properties of HPLC and GC that indicate their advantages and disadvantages in terms of flavonoid analysis
Source publication
Flavonoids are a class of secondary plant metabolites with low molecular weights. Most flavonoids are highly polar and unsuitable for gas chromatographic analyses. Derivatization is commonly used to make them amenable to gas chromatography by altering their physicochemical properties. Although highly effective, derivatization techniques introduce e...
Contexts in source publication
Context 1
... to the complexity of the sample extracts/mixtures, chromatographic separations are often combined with mass spectrometric detection, which provides qualitative information [10]. The advantages and disadvantages of using HPLC and GC for flavonoid analysis are summarized in Table 1. ...Context 2
... the predicted formulas for the derivatized species had to contain one and four Si atoms, respectively. By cross-checking the predicted formulas for the observed ions (Table 1), the list of likely molecular formulas was narrowed down to a single possible formula (third column in Table 2). This would not have been possible if derivatization was done with the traditional batch method, since the reaction was less efficient and only one hydroxyl group was derivatized. ...Similar publications
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