Figure 1 - uploaded by Jérémy Bouyer
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Principle of the release of insects carrying a dominant lethal gene (RIDL). (A) scheme of the transgene. The tetracycline activator variant (tTAV) protein binds to its own promoter, activates its own transcription and perturbs overall gene expression in the cells, resulting in mosquito death, unless tetracycline that binds and inactivates tTAV is provided. (B) During mass rearing in the production unit, mosquitoes develop normally in the presence of tetracycline. For an intervention, males are sorted at the pupal stage (based on the smaller size of male pupae). Once released, they mate with wild females whose progeny will die due to unrestricted tTAV activity.
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Background
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Citations
... The current management plan includes coordinated actions such as monitoring protocols and activities for stakeholders and local communities. The implementation of new control approaches, such as the sterile insect technique (SIT), Wolbachia-based strategies, pyriproxyfen autodissemination and transgenic technologies have recently been proposed [6,13]. SIT is a method of insect control with a strong record of successful applications against a range of agricultural insect pests [14,15]. ...
Aedes albopictus, an invasive mosquito species, is currently well established in many European countries, posing high risks to human health. A preliminary trial using repetitive releases of irradiated sterile male mosquitoes was designed, implemented and evaluated for the first time in Greece. The main scope of this trial was to investigate the efficacy of sterile insect technique (SIT) on wild population egg hatch rate in Greece using mass-produced sterile male mosquitoes from another country (Italy). The study was conducted in Vravrona area, close to Athens International Airport (Attica Region). The number of eggs laid in ovitraps was similar in all intervention and control plots. However, a significant reduction in egg hatch rate was recorded in the SIT plot in comparison with both control plots starting two weeks after the first release. This trial validates the logistics (transportation, releases handling and monitoring) as a major step towards implementing efficient, environmentally safe control approaches as an additional tool against the invasive Aedes species in Greece and more widely in Europe.
