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The recent outbreak of the coronavirus disease 2019 (COVID-19) pandemic and the continuous evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have highlighted the significance of new detection methods for global monitoring and prevention. Although quantitative reverse transcription PCR (RT-qPCR), the current gold standard...
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... pairs and extension probes were designed using Assay Design 4.1 software (Agena Bioscience, Inc., San Diego, CA, USA) and their specificities were confirmed using Primer-BLAST (https://www.ncbi.nlm.nih.gov/tools/primer-blast/). Both the multiplex PCR primer pairs and extension probes for single base extension (SBE) were synthesised by Tsingke Biotechnology (Beijing, China). The 17 genetic target regions and their respective primers and probes are listed in Table 1. ...Context 2
... new coupled method using a universal mass spectrometer can be integrated with other mass spectrometers of any brand, reducing the limitations of the original work using a closed Table 3 The interpretation criteria of the SARS-CoV-2 MS method results. 1 2 3 4 5 6 7 8 9 1 0 1 1 1 2 1 3 1 4 1 5 1 6 1 7 RNaseP N RdRp D950N T19I F486V T95I D614G A67V L452Q Q493R V213G D405N P681R T376A S704L a Y145D Delta ...