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Plantlet production on leaf discs of Peperomia 'Red R ipple' after 10 weeks culture as influenced by 2.5 m g/liter kinetin in com binations with 5.0 (A), 0.5 (B) and 0.05 (C) m g/liter NAA.
Source publication
This paper reports a method of propagating Peperomia ‘Red Ripple’3 from leaf discs in vitro .
Contexts in source publication
Context 1
... and single discs were placed in 20 x 150 mm disposable test tubes on a Schenk and Hildebrandt (2) salt medium con taining 30 g sucrose and 6 g/liter agar. Table 1). The high kinetin/NAA ratio consis tently promoted shoot initiation whereas increasing the level of NAA led to excessive callus development, root formation, and media discoloration (Fig. 1). NAA at 0.5 mg/liter only pro moted shoot initiation at the highest kinetin level (10.0 mg/liter). NAA at 5.0 mg/liter caused production of dark brown or black callus and roots regard less of the kinetin level and shoots rarely ...
Context 2
... wettable powder) for 15 min, 0.26% sodium hypochlorite for 10 min, and 3 changes of sterile distilled water for 5 min each. Each solution was stirred constantly to facilitate surface contact. The shoot tips were held in sterile distilled water for up to 30 min until they were cut under water to 1 to 2 cm in length and then placed into culture (Fig. 1A and ...
Context 3
... shoot proliferation. Trifoliate leaves formed during culture establish ment (Fig. 1C). After cultures were transferred to the agar medium, many of these leaves died. However, lateral buds grew, callus formed around the stem, and shoots grew out from the callus. Dense masses of 20 to 40 shoots formed within 4 weeks (Fig. ID). Since many lateral buds on these shoots developed, each shoot could be divided at least twice. ...
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Citations
... 2Present address: V irginia Cooperative Extension Ser vice, Box 3431, H am pton, VA 23669.The latter form an impervious film on the plant surface sometimes penetrating the stomatal aperture and forming water res istant plugs(1,3). ...
Leaf discs of Peperomia viridis Tre. were cultured on a Murashige and Skoog’s medium supplemented with 2 mg/liter kinetin and 1 mg/liter NAA. The addition of 100–500 mg/liter of raw ginseng powder increased shoots per explant and improved the quality of the regeneration plantlets.
... U nder postal reg ulations, this paper therefore must be hereby marked advertisement solely to indicate this fact. The latter form an impervious film on the plant surface sometimes penetrating the stomatal aperture and forming water res istant plugs (1,3). ...
... Growth retardants are frequently re quired for height control of Easter lily. Several chemicals are effective, however, ancymidol is generally regarded as the' best and is used most widely commercial ly (1,2,3,4,5,8,9). Ancymidol is usual ly applied postemergence as a soil drench or foliar spray. ...
Antitranspirants (Exhalt 4-10, Vapor Gard, Wilt Pruf, and Folicote) were more phyto-toxic when applied to detached florets than to detached leaves of hydrangea (Hydrangea macro-phylla Thunb. cv. Improved Merveille). Transpiration was high from hydrangea flowers as well as leaves. Folicote applied to intact flowering plants at concentrations of 1.25–5.00% was effective in reducing transpiration 10–15% compared to untreated plants.
The chapter covers mutation work (mutagens, working dose, mutants) carried out throughout the world on approximately 120 ornamental crops.
In recent years, propagation of numerous ornamental plants by tissue culture has become accepted commercial practice. Great progress has been made since Haberlandt (29) described the basic principles of plant tissue culture, followed by successful culture of plant callus by Nobecourt and Gautheret in France and by White in the U.S. (79). The rapid strides achieved in the field of micropropagation of ornamental plants have been made possible by the research accomplishments of myriad dedicated scientists. It is impossible to list all of these accomplishments in a brief overview, but some of the more significant contributions will be included in the following categories of ornamentals: orchids, ferns, foliage plants, bulbous species, herbaceous ornamentals, and trees and shrubs.
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IntroductionGeneral MethodologyPlant Regeneration MethodologyGenetic Regulation of Plant RegenerationConcluding RemarksLiterature Cited
