Plant material. (a) Diplotaxis acris (acc. 554488); (b) Diplotaxis assurgens (Delile) Thell. (acc. S17021002); (c) Diplotaxis berthautii Braun-Blanq. and Maire (acc. S17021001); (d) Diplotaxis brachycarpa (acc. S17021009); (e) Diplotaxis brevisiliqua (Coss.) Mart.-Laborde (acc. S17021010); (f) Diplotaxis duveyrieriana Coss. (cretacea) (acc. S17021008); (g) Diplotaxis eruicoides (L.) DC. (acc. DIPLO2); (h) Diplotaxis harra (acc. 338167); (i) Diplotaxis ibicensis (Pau) Gómez-Campo (acc. S17021006); (j) Diplotaxis ilorcitana (acc. S17021007); (k) Diplotaxis muralis (L.) DC. (acc. DIPLO 5); (l) Diplotaxis siifolia Kunze (acc. S17021003); (m) Diplotaxis simplex (Viv.) Spreng. (acc. S17021004); (n) Diplotaxis tenuifolia (L.) DC. (acc. DIPLO 12); (o) Diplotaxis tenuisiliqua (acc. DIPLO 10); (p) Diplotaxis viminea (L.) DC. (acc. S17021005); (q) Diplotaxis virgata (Cav.) DC (acc. CM 0025909). The Royal Botanical Garden Kew (Richmond, United Kingdom) provided (a) and (h). The Universidad Politécnica de Madrid UPM (Madrid, Spain) provided (b-f,i,j,l,m,p). The Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, IPK (Gatersleben, Germany) provided (g,k,n,o). The Universidad de Castilla-La Mancha UCLM (Ciudad Real, Spain) provided (q). All images are on the same size scale.

Plant material. (a) Diplotaxis acris (acc. 554488); (b) Diplotaxis assurgens (Delile) Thell. (acc. S17021002); (c) Diplotaxis berthautii Braun-Blanq. and Maire (acc. S17021001); (d) Diplotaxis brachycarpa (acc. S17021009); (e) Diplotaxis brevisiliqua (Coss.) Mart.-Laborde (acc. S17021010); (f) Diplotaxis duveyrieriana Coss. (cretacea) (acc. S17021008); (g) Diplotaxis eruicoides (L.) DC. (acc. DIPLO2); (h) Diplotaxis harra (acc. 338167); (i) Diplotaxis ibicensis (Pau) Gómez-Campo (acc. S17021006); (j) Diplotaxis ilorcitana (acc. S17021007); (k) Diplotaxis muralis (L.) DC. (acc. DIPLO 5); (l) Diplotaxis siifolia Kunze (acc. S17021003); (m) Diplotaxis simplex (Viv.) Spreng. (acc. S17021004); (n) Diplotaxis tenuifolia (L.) DC. (acc. DIPLO 12); (o) Diplotaxis tenuisiliqua (acc. DIPLO 10); (p) Diplotaxis viminea (L.) DC. (acc. S17021005); (q) Diplotaxis virgata (Cav.) DC (acc. CM 0025909). The Royal Botanical Garden Kew (Richmond, United Kingdom) provided (a) and (h). The Universidad Politécnica de Madrid UPM (Madrid, Spain) provided (b-f,i,j,l,m,p). The Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, IPK (Gatersleben, Germany) provided (g,k,n,o). The Universidad de Castilla-La Mancha UCLM (Ciudad Real, Spain) provided (q). All images are on the same size scale.

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Article
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Nuclear and cytoplasmic DNA barcoding regions are useful for plant identification, breeding, and phylogenesis. In this study, the genetic diversity of 17 Diplotaxis species, was investigated with 5 barcode markers. The allelic variation was based on the sequences of chloroplast DNA markers including the spacer between trnL and trnF and tRNA-Phe gen...

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Context 1
... plant material consisted of 17 Diplotaxis species retrieved from different European genebanks (Figure 1) including the Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung, IPK (Gatersleben, Germany), the Royal Botanical Garden Kew (Richmond, United Kingdom), the Universidad Politécnica de Madrid UPM (Madrid, Spain), and the Universidad de Castilla-La Mancha UCLM (Ciudad Real, Spain). mg of fresh leaves were collected, followed by nucleic acid isolation using the DNeasy plant mini kit (Qiagen, Hilden, Germany). ...

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