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Piwi expression during zebrafish development The embryonic development of zebrafish takes 48-72 hours post fertilization (hpf) and can be roughly divided in six periods: zygote, cleavage, blastula, gastrula, segmentation, pharyngula and hatching. After leaving the egg, the larva develops to sexual maturity after 12-16 weeks (green box, 1:10 scale compared to previous images). The Piwi protein ZIWI can be observed throughout development (Houwing et al. 2007). ZILI was found to be expressed from 72 hpf onwards (Houwing et al. 2008) (dark blue line), but Sun and described ZILI already in the one-cell stage of the embryo (Sun et al. 2010) (light blue dashed line). However, Sun describes a cap of expression of ZILI around 24 hpf. The image was adapted from (Kimmel et al. 1995).  

Piwi expression during zebrafish development The embryonic development of zebrafish takes 48-72 hours post fertilization (hpf) and can be roughly divided in six periods: zygote, cleavage, blastula, gastrula, segmentation, pharyngula and hatching. After leaving the egg, the larva develops to sexual maturity after 12-16 weeks (green box, 1:10 scale compared to previous images). The Piwi protein ZIWI can be observed throughout development (Houwing et al. 2007). ZILI was found to be expressed from 72 hpf onwards (Houwing et al. 2008) (dark blue line), but Sun and described ZILI already in the one-cell stage of the embryo (Sun et al. 2010) (light blue dashed line). However, Sun describes a cap of expression of ZILI around 24 hpf. The image was adapted from (Kimmel et al. 1995).  

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