Figure 2 - available via license: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Content may be subject to copyright.
Download
View publication
Pipetting emulsion onto the oil phase (A) Lipids in oil is added on top of the diluted External buffer. (B) Emulsion is pipetted onto the oil phase. (C) A resultant solution with phases. The solution is centrifuged to form liposomes at the next step.

Pipetting emulsion onto the oil phase (A) Lipids in oil is added on top of the diluted External buffer. (B) Emulsion is pipetted onto the oil phase. (C) A resultant solution with phases. The solution is centrifuged to form liposomes at the next step.

Source publication
Figure 1. A paint brush washed with the sucrose solution.
Figure 2. Pipetting emulsion onto the oil phase (A) Lipids in oil is...
Figure 3. A unit spaced with silicon spacer for microscopic observation...
Figure 4. A representative result obtained by this protocol (16 h...
Activation of a diluted E. coli cell-free transcription-translation system within liposomes by hypertonic concentration
Article
Full-text available
  • Dec 2021
We present a protocol for activating protein synthesis in liposomes encapsulating a diluted E. coli cell extract-based TX-TL (transcription-translation) system by hypertonic concentration. Protein expression is turned on in the liposome-encapsulated TX-TL system by simple treatment with a concentrated external solution. The expression of sfGFP is d...
Cite
Download full-text

Contexts in source publication

Context 1
... Carefully add 50 mL of the lipid solution (prepared in step 1) on top of the diluted External buffer with pipette ( Figure 2). d. ...
Context 2
... Add 50 mL of the emulsified solution (prepared in step 2) onto the oil phase with the lipids (Fig- ure 2). ...

Citations

Controlling the Periodicity of a Reaction–Diffusion Wave in Artificial Cells by a Two-Way Energy Supplier
Article
  • Oct 2022
  • ACS NANO
Reaction-diffusion (RD) waves, which are dynamic self-organization structures generated by nanosize molecules, are a fundamental mechanism from patterning in nano- and micromaterials to spatiotemporal regulations in living cells, such as cell division and motility. Although the periods of RD waves are the critical element for these functions, the development of a system to control their period is challenging because RD waves result from nonlinear physical dynamics under far-from-equilibrium conditions. Here, we developed an artificial cell system with tunable period of an RD-driven wave (Min protein wave), which determines a cell division site plane in living bacterial cells. The developed system is based on our finding that Min waves are generated by energy consumption of either ATP or dATP, and the period of the wave is different between these two energy suppliers. We showed that the Min-wave period was modulated linearly by the mixing ratio of ATP and dATP and that it was also possible to estimate the mixing ratio of ATP and dATP from the period. Our findings illuminated a previously unidentified principle to control the dissipative dynamics of biomolecules and, simultaneously, built an important framework to construct molecular robots with spatiotemporal units.
View