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Aniseed plant (Pimpinella anisum Linn.) is a flowering medicinal plant and are annual important spices belonging to the family of Apiaceae native to eastern Mediterranean region and Southwest Asia. Aniseed (Pimpinella anisum Linn.) essential oil has been widely used in aromatherapy for breathing difficulties as well as has a good effect on asthma a...
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Citral is a widely used monoterpene aldehyde in aromatherapy, food and pesticide industries. A new validated reverse phase high performance liquid chromatography (RP - HPLC) procedure for the detection and quantification of cis - trans isomers of citral was developed. The RP ˗ HPLC analysis was carried out using Enable C - 18G column (250 × 4.6 mm,...
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... trans-anethole) in proportion to 72.49%, limonene 10.01%, anisole 5%, and α-pinene (3.263%). These results are consistent with those of other authors of the domain, who identified trans-anethole, a phytoestrogen, as the major compound in Pimpinella anisum essential oil [32][33][34][35][36]. ...
Citation: Dumitrescu, E.; Muselin, F.; Tîrziu, E.; Folescu, M.; Dumitrescu, C.S.; Orboi, D.M.; Cristina, R.T. Pimpinella anisum L. Essential Oil a Valuable Antibacterial and Antifungal Alternative. Plants 2023, 12, 2428. https://doi.org/10.3390/ plants12132428 Academic Editors: Milan S. Stankovic and Christophe Hano Abstract: Anise (Pimpinella anisum L.) essential oils are intensely investigated worldwide for the beneficial properties, due to the specific bioactive compound's structure. (1) Background: This study characterized the structure of the Pimpinella anisum essential oil and evaluated its antimicrobial properties. (2) Methods: An evaluation of the antibacterial and antifungal activity targeted strains of Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus aureus (ATCC 25923), Streptococcus pyogenes (ATCC 19615), and levure Candida albicans (ATCC 10231). Gas chromatography coupled with mass spectrometry (GC/MS) was used for structure identification, and the optical density mass loss was applied for the analysis of different dilutions of aniseed essential oils antimicrobial activity. (3) Results: A total of 13 compounds were identified, of which trans-anethole was in the highest proportion (72.49%), followed by limonene (10.01%), anisole (5%), and α-pinene (3.26%). The results obtained and statistically analyzed, utilizing one-way ANOVA with Bonfer-roni's multiple comparison test, indicated the antimicrobial activity (p < 0.001) of anise essential oil. (4) Conclusion: Anise essential oil is a promising phyto-remedy with important antimicrobial activity against both Gram-positive and Gram-negative pathogens. Inhibition high percentages were found for the P. aeruginosa and S. aureus strains, but also excellent antifungal activity against C. albicans was ascertained.
... Yadav et al. [22] 2.55 n.a. Albulushi et al. [23] 0.3927 Clearly soluble at any volume n.a.: not available provided at the paper. ...
strong>ABSTRACT. Anise seeds can be processed into essential oils by steam distillation and solvent extraction. This research aims to produce a high oil yield by determining the optimum conditions of extraction, extracting anise seeds according to optimum conditions, producing anise oil with good quality based on 90% alcohol solubility analysis, analyzing the acid number, and conducting GC-MS analysis. The Activity Method was carried out by determining the optimum conditions of extraction on the effect of the variation in the ratio between anise seed powder and petroleum ether. Furthermore, based on optimal conditions, extraction was carried out at a temperature of 60°C, 5 hours with 5 repetitions. The results of essential oils were analyzed including solubility analysis, oil acid number, and GC-MS. The optimal extraction conditions were obtained at the ratio of anise seeds and petroleum ether 1:12.5 with an average oil yield of 4.72%. The results of the analysis showed that anise oil had good quality based on the oil solubility in 90% alcohol with a 1:7 ratio. The acid number of anise seed oil was obtained at 1.14444. GC-MS analysis showed that anise seed oil contained estragol, anethole, limonene, fenchone, and anis ketone.
... Haşimi et al. [46] determined that the main components of anise essential oil were trans-anethole (52.94%), followed by isoanethole (13.89%), caryophyllene oxide (8.55%), and caryophyllene (2.4%). Mohammed et al. [47] reported that essential oil had a percentage of trans-anethole of 55.491%. ...
The two-spotted spider mite, Tetranychus urticae, also known as the red spider, is one of the most harmful pests in agriculture and causes large losses of many crops. These mites have rapidly developed a resistance to many chemical pesticides in recent years. In this study, the essential oil of seeds of the anise plant (Pimpinella anisum) was extracted by hydrodistillation, and the chemical composition of the oil was analyzed. The antioxidant activity of the volatile oil was determined by the DPPH radical scavenging assay. The acaricidal activity of the anise oil, a natural acaricide, was evaluated for its ability to protect green bean plants from mite injury. The two-spotted spiders were spread on green bean seedlings for 1 week; then, different plants were sprayed with different concentrations of anise oil (10, 20, 30, or 40 µL/L). Our results revealed that anethole was the major component of anise oil, at 53.23%. The acaricidal effect of the various concentrations on T. urticae was recorded after 24, 48, and 72 h of treatment. Our findings suggest that anise oil showed significant acaricidal activity against T. urticae in a dose- and time-dependent manner. Anise oil at a concentration of 40 µL/L killed 96.0% of the red spiders after 72 h. Also, all concentrations of anise oil inhibited acetylcholinesterase, and the spiders’ protease activity declined when the plants were treated with 30 or 40 µL/L of anise oil. The concentrations of 10 and 20 µL/L did not significantly affect the protease activity of T. urticae mites. We can conclude that anise oil exhibited acaricidal activity against T. urticae and that this was highly correlated with the inhibition of acetylcholinesterase and protease activities in the mites.
... Anise (Pimpinella anisum L.), belonging to the Umbelliferae (Apiaceae) family with around 300 genera and 3000 species, is an annual aromatic herb [1,2]. Anise is a plant with natural distribution in the Mediterranean basin and Southwest Asia [1,3]. ...
... Anise (Pimpinella anisum L.), belonging to the Umbelliferae (Apiaceae) family with around 300 genera and 3000 species, is an annual aromatic herb [1,2]. Anise is a plant with natural distribution in the Mediterranean basin and Southwest Asia [1,3]. It is an herbaceous plant with a height of about 90 cm. ...
... The plant with umbellate inflorescences has flowers with approximately 3 mm in diameter and white in color. Schizocarp fruits are 3-5 mm long [1,4]. ...
... High averages of peroxide were reported in the study of Moser and Vaughn, (2012), in anise seeds with the values of 10.3 meq.O2/kg; and cumin seed oil 15.3 meq.O2/kg, thus, could be attributed to the differences in climate, cultural practices and post harvest factors. A similar trend of results was indicated in the study of Albulushi et al.(2014) andSalim et al.(2016) in their determination of acid value in anise seed oil (1.29 and 0.39, respectively). ...
Fixed oils (lipids) from the seeds of Anise (Pimpinella anisum L.), Cumin (Cuminum cyminum L.), Fennel (Foeniculum Vulgare L.) and Caraway (Carum carvi L.) were extracted by the cold press. The oil content, chemical and physical properties, as well as, minerals content were determined. Results approved that the oil content of the examined samples was at the range of 12.5-17.16%.; the most dominant fatty acids were petroselinic acid (18:1-δ-6), which is a characteristic fatty acid of the Apiaceae family (anise, cumin, fennel, and caraway) at the range of 55.43 to 79.3%, followed by linoleic acid (13.16 to 331.71%.). The obtained results from free fatty acid (FFA) and acid value (AV) found to be the lowest in anise seed oil (1.6%, 0.82 mg KOH/g oil, respectively); while, caraway seed oil had the highest values of PV and viscosity at 25 C (3.7 meq. O2/kg oil, 85.24 Cp, respectively). Chlorophyll and density measured from 0.09-0.43 mg/kg and 0.93-0.96 g/cm3. In this study, the mineral content of the Apiaceae plant s oils is detected by Emission Scanning Electron Microscopy (FE-SEM). Cumin seed oil had the lowest value of sulfur and carbon (0.04, 68.65, respectively). Aluminum and Bromine ranged from 0.79-20.5 and 1.47-14, respectively. The percentage of inhibition of caraway, cumin, Anise, and Fennel fixed oil in the linoleic acid system were 78.87, 75.31, 60.26, and 59.11%, respectively, compared with Standard synthetic antioxidant BHT which was 92.07%.
... In our investigation, the concentration of trans-Anethole in aniseed was almost same as in the Gende et al. (2009)and Acimovic et al., 2015 study. Moreover, trans-Anethole identified by (Ullah et al.,2013;Ullah et al.,2014;Hasimi et al.,2014;Mohammed et al.,2014). While Gerogiannaki and Masouras(2015) reported that trans-Anethole and isomer cis-anthole are main in aniseed seed. ...
The essential oils were isolated from dried Pimpinella anisum L. seeds by Cleavenger aperture extraction, and
fractions were identified by gas chromatography-mass spectrometry (GC-MS). 51 components were detected in
aniseed. The components were mainly 19 sesquiterpenes (37.25 % ), ten monoterpene (19.60%) , tow diterpene
(3.92%) and one hemiterpene (1.96 %). The major components identified in the essential oil of Pimpinella
anisum was trans-Anethole or Anisole (71.52 %) , benzocycloheptene (6.59 %),and Isoeugenyl acetate ( 4.63 %).
In addition 44 components were present at less than 1%.
Bu çalışmada, farklı genotiplerin (populasyon) anasonun (Pimpinella anisum L.) verim ve verim bileşenleri üzerindeki etkisini ortaya çıkarmak amaçlanmıştır. Deneme, Eskişehir ekolojik koşullarında 2017 ve 2018 yılları yetiştirme döneminde gerçekleştirilmiştir. Çalışmada Türkiye’nin farklı bölgelerinden temin edilen dört farklı anason populasyonu (Afyon, Bilecik, Çeşme ve Burdur) kullanılmıştır. Bitki materyalleri, tesadüf blokları deneme desenine göre üç tekerrürlü olarak yetiştirilmiştir. Sonuçlar, populasyonların 1000 tohum ağırlığı (g) hariç incelenen diğer tüm parametrelerde önemli farklılığa sahip olduğunu göstermiştir. Bilecik ve Çeşme populasyonlarından en yüksek tohum verimi (sırasıyla 80.56 ve 76.31 kg da-1) ve en yüksek uçucu yağ verimi (sırasıyla 2.17 ve 2.22 L da-1) elde edilmiştir. Diğer taraftan çalışmada elde edilen uçucu yağ oranı %2.67-2.92 arasında değişmiş, Avrupa farmakopesinde belirtilen % 2 sınırının üzerinde olduğu görülmüştür. Netice olarak; çalışmada yer alan populasyonlar arasında yüksek tohum ve uçucu yağ verimleri açısından Bilecik ve Çeşme populasyonlarının öne çıktığı belirlenmiştir.
Essential oils (EOs) from anise seeds (Pimpinella anisum) collected in the Algerian region were extracted by microwave‐assisted hydrodistillation (MAHD) and hydrodistillation (HD). The oil yield (%), chemical composition, energetic consumption and the antimicrobial properties were evaluated of the obtained EO. The yields obtained by both processes were 2.81 ± 0.01% for 90 minutes and 3.30 ± 0.05% for 330 minutes for MAHD and HD, respectively. The determination of the chemical composition of EOs by GC‐MS revealed the presence of a majority compound, trans‐anethole 81.52% and 91.31% for MAHD and HD, respectively. The other main compounds present in the oils for MAHD and HD were, respectively: methyl chavicol (estragole) (0.63%‐1.39%), cis‐anethole (0.23%‐0.34%), anisaldehyde (1.1%‐1.57%), anisyl methyl ketone (0.28%‐0.13%), trans‐methyl isoellgenol (0.31%‐0.17%), γ‐himachalene (0.5%‐0.36%), trans‐pseudoisoeugenyl 2‐methylbutyrate (6.53%‐2.53%) and epoxy‐pseudoisoeugenyl 2‐methylbutyrate (2.01%‐0.56%). The EO obtained by MAHD detected other compounds, fenchone 0.41%, limonene 0.68%, cis‐jasmolacatone extra C 0.41%, trans‐isolongifolanone 0.2% and α‐himachalene 0.16%. The energetic consumption by MAHD was significantly less than HD. Theoretical data from mathematical modelling were compared with practical data, and the coefficient of determination was between 0.93 and 0.97. The sensibility of bacterial strains to the essential oil obtained by MAHD is marked relatively to that obtained by HD, and for the sensibility of fungal strains, it was conserved. The MAHD process revealed a good yield of anise seeds EOs while reducing extraction time and energetic consumption. The EO extracted by this process revealed the presence of other compounds while improving antibacterial activity.
Recently, there has been an increasing demand for bioactive compounds from plants that can be used in the pharmaceutical and nutraceutical industry. This is due to their presumed roles in preventing diseases such as cancer and their ability enhance human skin to make it look younger. Along with conventional methods such as liquid–liquid extraction, solid-phase extraction, and solid-phase micro-extraction, numerous newer techniques have been developed to enhance the extraction of bioactive compounds from their plant sources in a greater scale. These techniques include ultrasound-assisted extraction, pressurized liquid extraction, subcritical water extraction, supercritical fluid extraction, microwave-assisted extraction, and instant controlled pressure drop extraction. This review describes the types of plants that are widely known for their beneficial effects on human skin and the different extraction techniques—along with their basic mechanisms, advantages, and disadvantages—for extracting bioactive compounds from plants.
Anise (Pimpinella anisum L.) is a herbaceous annual plant from Apiaceae family. It originates from the Mediterranean region where it is quite widespread. On the other hand, star anise (Ilicium verum Hook.) is an evergreen perennial plant from Illiciaceae family. The cultivation of the plant is restricted to south China and Vietnam. Fruit with seed is used from anise, while fruit without seed is used from star anise. The fruit of star anise derives its name from the characteristic star shape. The seed of this plant is rich in essential oil. Both species contain essential oil both in the seed as well as in the fruit, anise approximately 1.7%, star anise from 4.3 to 6.7%. The dominant component in essential oils of both species is trans-anethole (93.2-96.4%).
