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Medicinal plants are of great importance to researchers in the field of pharmacology as most pharmaceutical industries depend on medicinal plant for their raw materials. Hibiscus asper belongs to the family Malvaceae and is well known for its medicinal properties. The present study was carried out to evaluate the antioxidant effect and p...
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... screening of aqueous methanol fraction of Hibiscus asper leaf revealed the presence of alkaloids, flavonoids, saponins, tannins, phenols, steroids, and terpenoids as shown in Table 1. Values are present as mean ± SD. "+" indicates present ...Similar publications
Diabetes is a chronic disease caused by insufficient insulin production or the inability of the body to use the insulin produced. Hibiscus surattensis L. is a plant species from the genus Hibiscus (Malvaceae), with leaves that has been used in traditional medicine to treat diabetes. The ethanol leaf extract of Hibiscus surattensis (Linn.), was eval...
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... The antifungal potential of the plant extract was performed by agar disc diffusion method as reported by [20,21]. ...
This research evaluated GC-MS profile, and pathogenic effect of methanolic leaves extracts of Khaya anthotheca on Some Selected fungi. Khaya anthotheca a member of Meliaceae family is traditionally used for treating several ailments. The leaves were harvested in Sukundi road Wukari and air dried for four weeks, the leaves were pulverized into powder using manual blender and stored in an air tight container. Extraction was done with absolute methanol; the extract was filtered using mesh and the filtrate was concentrated at 68oC. Only two grams of the filtrate were used for the GC-MS study. One-way analysis of variance (ANOVA) was used for statistical analysis. The GC-MS result revealed 50 constituents. Result of Methanolic extracts of leaves of Khaya anthotheca only showed significant activity against Aspergillus niger at dose100 µg/mL, Candida tropicalis and Fusarium oxysporium at dose 500 µg/mL. This study supports the use of Khaya anthotheca by indigenous herbalists by providing scientific proof of the plant's ability to treat pothegenic diseases. To establish the effectiveness of this plant in the management of fungal effections, more clinical trials at the clinical levels are needed.
... Aculeata) contain an array of organic compounds, tannins, avonoids, catechins, steroids, triterpenes, and saponins offering various applications 11 . Hibiscus Asper leaves contains several bioactive compounds, with the predominant been 9,12,15-octadecatrien-1-ol, n-Hexadecanoic acid, octadecatrienol acid, methyl palmitate, and phytol with wide industrial and medical applications 12 . ...
... Twelve (12) grams each of the different plant specimens were weighed and transferred into a beaker containing 100 ml of methanol. Methanol is predominantly used for the extraction of phytochemicals from plant specimen because of its high yield attributed to its polarity, and high volatile nature 45,62-64 . ...
This work presents a phytochemical study of selected plants extracts to evaluate their potential synergistic corrosion inhibition performance. It was observed that the dominant phytochemicals derived from ten plant leaves and seeds can be synergies in group to enable better understanding of their inhibitory mechanism, which is a potentially significant gap in knowledge. Based on the results of phytochemical screening using FTIR, GC-MS, VU-VIS, and further analysis of the active and predominant phytochemicals, their reaction complex, inhibitor compatibility and other physical and chemical properties as reported in literature, the plant extracts have been grouped into three Organic Green Corrosion inhibitor (OGCI) formulations. Group 1-Greentreat-1234, Group 2-Greentreat-95627 and Group 3-Greentreat-85. The inhibitor adsorption is via, Pie -bond orbital adsorption, Electrostatic adsorption, Chemisorption and Physisorption as reported in literature. The implication of this study is that OGCI has the potential to control wet corrosion of carbon steel.
... By comparing a query mass spectrum with the reference data in a spectrum-matching library, the NIST database was used to interpret the GC-MS data. GC-MS analysis of Hibiscus asper leaves revealed phytochemical profiles that included flavonoids, tannins, phenols, saponins, alkaloids, glycosides, terpenoids, and steroids, along with 23 bioactive compounds in the aqueous methanol fraction, including phytol, n-hexadecanoic acid, octadecatrienol acid, methyl palmitate, and octadecatrienol acid [37,38]. Ten chemicals were identified in the chloroform-methanol extract of Rhazya stricta by Baeshen et al. [39]. ...
Globally, healthcare is concerned about the rising prevalence of type 2 diabetes. Phytochemicals from medicinal plants have shown great promise in improving human health. The present study aimed to determine the secondary metabolites of Ziziphus oenopolia (L.) fruit extract that contribute to its anti-diabetic activity. The anti-diabetic properties were assessed by in vitro and in silico approaches using α-amylase and α-glucosidase inhibitory assays. Gas chromatography and mass spectroscopy analyses were used to profile Z. oenopolia fruit contents, and a total of four bioactive chemicals and eight phytocompounds were tentatively identified, including flavonoids, terpenoids, phenols, steroids, tannins, and saponins. The Z. oenopolia fruit hydroalcoholic extract inhibits α-amylase and α-glucosidase enzymes in a dose-dependent manner (IC50 = 328.76 and 337.28 µg/mL, R ² = 0.979 and 0.981). Additionally, phytochemicals found in Z. oenopolia fruit exhibit the ability to inhibit anti-diabetic targets, specifically α-amylase and α-glucosidase (2QV4 vs 3A4A; correlation coefficient, r = 0.955), as demonstrated by computational analysis. This establishes the fruit as a promising and environmentally friendly option for treating hyperglycemia, highlighting the positive correlation between anti-diabetic objectives.
... The GC-MS analysis of Aristolochia tagala leaf extracts revealed the presence of 42 compounds across various solvents such as petroleum ether, chloroform, ethyl acetate, methanol, and hydro-alcoholic (Mariyammal et al., 2023). Olivia et al., utilized GC-MS analysis to identify twenty-three bioactive compounds in the hydromethanolic fraction of Hibiscus asper leaves such as 9,12,15-octadecatrien-1-ol, n-Hexadecanoic acid, octadecatrienol acid, methyl palmitate and phytol were significant phytoconstituents (Olivia et al., 2021). Lupeol and phytosterols possess various properties like antifungal, anti-inflammatory, antibacterial, anti-tumor, antioxidant and anti-ulcerative effects which plays their multifunctional biological roles (Ito et al., 2017). ...
... The range of fragments varies in size from 40 to 600 Da. The component spectrums were correlated to a database of known spectrums recorded in the GC-MS NIST library [57,58]. ...
... The carrier gas was helium with column velocity flow of 1.0 L/min, ion-source temperature was 250 C, interface temperature was 300 C, operating pressure was 16.2 psi, out time was 1.5 min, injection temperature was 300 C in split mode at 1 µL injector, while the temperature of the column was initially 50 C for 5 min and raised to 250 C at the rate of 20 C/min for 5 min (Ukwubile et al., 2019). The total elution time was 25 min, and each compound was calculated in terms of relative abundance, peak areas and retention times while the identification of compounds was done by comparing with data from the NIST library (Olivia et al., 2021). ...
BACKGROUND AND OBJECTIVES: _Camellia sinensis_ is a tree popularly called green tea used as a strong antioxidant and for treating diseases such as cancer. This present study was aimed at assessing the phytochemicals and evaluating the hepatoprotective activity of leaf extract in Wistar rats. METHODS: The phytochemical screening was performed by standard methods, GC-MS analysis was carried out in Agilent 7890 A GC coupled to a MS detector, acute toxicity study was evaluated by the OECD guidelines, and hepatoprotective activity were evaluated in randomly groups of five rats of six rats in paracetamol-induced liver toxicity, while liver biomarkers were evaluated using automated methods. RESULTS: The phytochemical screening of leaf extract revealed the presence of major metabolites. The GC-MS analysis showed 18 bioactive compounds which are mainly fatty acids with retention times between 7.436 and 18.462 min. A high total phenolic content (TPC) of 1425.22 mg GAE/g and a total flavonoid content (TFC) of 802.01 mg QE/g were obtained. The acute toxicity did not produce any sign of toxicity at a dose of 5,000 mg/kg of extract. The extract produced a dose-dependent activity on the liver. These protections were statistically significant (p<0.05) when compared to the standard drug silymarin. There were no elevated values in liver function biomarkers in all the animal groups as well as histopathological damages to the liver after eight weeks of treatment at 200 and 400 mg/kg b.w. of extract doses. CONCLUSION: In conclusion, the study showed that _C. sinensis_ methanol leaf extract contains important phytochemicals that are protective against paracetamol-induced liver injuries, thus, the plant was considered safe for use as ethnomedicinal remedies for liver disease in traditional medicine.
... [5,6] The finding of active compounds from natural sources is the initial step in the development of new therapeutics. [7] For this, scientists are more interested in investigating secondary metabolites derived from plants to create novel therapeutic medications. In accordance with the World Health Report on Infectious Diseases (2000), combating bacterial resistance is one of the most significant problems for WHO in the current era. ...
Background: Background: Psychotria dalzellii Hook.f. (Rubiaceae) is an ethnobotanically important plant species, traditionally the stem part is used as a remedy for pruritus and the leaf juice is utilized in the preparation of eye drops for migraines. However, these claims lack of scientific validation. The current study focused on Gas Chromatography-Mass Spectrometry analysis and in vitro evaluation of antibacterial properties of leaf and stem bark acetone extracts of P. dalzellii. Materials and Methods: Materials and Methods: P. dalzellii leaf and stem-bark samples were collected from Joida, Western Ghats, Karnataka, India. The collected plant materials were subjected to Soxhlet extraction using acetone. The presence of phytochemicals were investigated by preliminary qualitative screening and GC-MS for quantitative estimation. Then, the antibacterial potential was analysed by agar well diffusion technique against selected plant and animal pathogenic bacterial strains. Results: Results: GC-MS chromatogram of acetone stem-bark extract shows the presence of 15 chemical constituents in that, Resorcinol (58.36%) and Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester (8.81%) were found to be the major phytoconstituents. Whereas, the leaf extract shows 19 bioactive compounds in that, 3,7,11,15-Tetramethyl-2-hexadecen-1-ol (26.99%); Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester (21.99%) and Resorcinol (17.87%) were found to be the major compounds. P. dalzellii extracts of stem-bark and leaf exerted greater antibacterial activity towards all tested bacterial strains. Among leaf and stembark extracts, the stembark acetone extract were shown to be the most potent against Gram-negative pathogenic bacterial strains like Pseudomonas syringae (19.33 ± 0.16 mm), Klebsiella pneumoniae (19.25 ± 0.14 mm), Xanthomonas compestris (18.16 ± 0.16 mm) and Escherichia coli (17.25 ± 0.14 mm). Conclusion: Conclusion: This study provides new insights into the Psychotria dalzellii as a potential tool for antibacterial drug discovery through in vitro investigation that could be used to treat human and plant infectious diseases.
... The carrier gas was helium with column velocity flow of 1.0 L/min, ion-source temperature was 250 C, interface temperature was 300 C, operating pressure was 16.2 psi, out time was 1.5 min, injection temperature was 300 C in split mode at 1 µL injector, while the temperature of the column was initially 50 C for 5 min and raised to 250 C at the rate of 20 C/min for 5 min (Ukwubile et al., 2019). The total elution time was 25 min, and each compound was calculated in terms of relative abundance, peak areas and retention times while the identification of compounds was done by comparing with data from the NIST library (Olivia et al., 2021). ...
BACKGROUND AND OBJECTIVES: Camellia sinensis is a tree popularly called green tea used as a strong antioxidant and for treating diseases such as cancer. This present study was aimed at assessing the phytochemicals and evaluating the hepatoprotective activity of leaf extract in Wistar rats. METHODS: The phytochemical screening was performed by standard methods, acute toxicity study and hepatoprotective activity were evaluated in randomly groups of five rats or six rats in paracetamol-induced liver toxicity. RESULTS: The phytochemical screening of leaf extract revealed the presence of major metabolites. The GC-MS analysis showed 18 bioactive compounds which are mainly fatty acids with retention times between 7.436 and 18.462 min. A high total phenolic content (TPC) of 1425.22 mg GAE/g and a total flavonoid content (TFC) of 802.01 mg QE/g were obtained. The acute toxicity did not produce any sign of toxicity at a dose of 5,000 mg/kg of extract. The extract produced a dose-dependent activity on the liver. These protections were statistically significant (p<0.05) when compared to the standard drug silymarin. There were no elevated values in liver function biomarkers in all the animal groups as well as histopathological damages to the liver after eight weeks of treatment at 200 and 400 mg/kg b.w. of extract doses. CONCLUSION: In conclusion, the study showed that C. sinensis methanol leaf extract contains important phytochemicals that are protective against paracetamol-induced liver injuries, thus, the plant was considered safe for use as ethnomedicinal remedies for liver disease in traditional medicine.
... FT-IR helps in assessing the functional groups present in a compound which can be related to the chemical and physical characteristics of the compound [25]. GCMS is applied widely in forensics, drug discovery and medicine, and environmental monitoring by examining each component independently after gas chromatography has separated the individual components of the mixture [26]. ...
Bioactive compounds and other constituents of plants have been shown to vary by cultivation region, species, environmental conditions and method of extraction among others. Phytochemical analysis of Cucurbita pepos farmed in Kiambu County, Kenya, or their seeds has not been documented. The present research aimed to bridge this knowledge gap by screening phytochemicals and characterizing the seed extracts of Cucurbita pepo cultivated in Kiambu County, Kenya. Cucurbita pepo seeds extracted using organic solvent extraction method employing methanol and preconcentrated in a vacuum rotatory evaporator. The extracts were characterized by GCMS and Fourier transform infrared (FTIR) techniques. Phytochemical analysis of the seeds revealed the presence of flavonoids, alkaloids, saponins, cardiac glycosides, and steroids. FT-IR analysis showed significant peaks for C–N, N–H, C–O, C–H, and CH3 functional groups. The GCMS studies revealed a significant number of fatty acids and their derivatives with 12-cis-octadecadienoate being the most abundant in the oil (53.93 %). A significant amount of the macrocyclic lactone 7,9-ditert-butyl-1-oxaspiro [4.5] deca-6,9-diene-2,8-dione (0.58 %) in the seeds was reported. Macrocyclic lactones are generally a class of anthelminthic drugs. These reported biologically active compounds have a wide range of medicinal and nutritional value. One interesting compound from the GCMS analysis of the seed extracts analyzed was the macrocyclic lactone providing a basis for further research on the anthelminthic actions of the seeds.
... The total elution time was 32.67 min with a 5 min solvent delay. Identification of phytochemicals present in the test samples was based on comparisons of their relative retention time (min) and mass spectra with a spectral database of known compounds obtained from the National Institute of 25 Standards and Technology (NIST) library . ...
Background: Natural products or functional supplements from edible plants have served as an attractive strategy for weight loss. Solanum macrocarpon L. (Solanaceae) and Abelmoschus esculentus (L.) Moench have been used in indigenous medicine for weight reduction and to treat several diseased conditions. This study evaluates the potential of S. macrocarpon and A. esculentusin weight reduction and lipid metabolism as well as revealing their phytochemical profile. Methods: Thirty-six rats were randomly divided into six groups of six rats each. Group 1 rats were fed with a 100% standard rodent diet (StD) throughout the experimental period. Rats in groups 2 to 6 were fed with a high-fat diet (HF-StD) for 4 weeks to induce obesity. Following obesity induction, the HF
