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Phylogram of RAxML analysis based on combined LSU and ITS sequence dataset. Bootstrap support values for maximum likelihood equal to or greater than 50%, and Bayesian posterior probabilities equal to or greater than 0.95 BYPP are indicated at the nodes as ML/ BYPP. The tree is rooted to Connersia rilstonii (CBS 537.74) and Pleuroascus nicholsonii (CBS 345.73). Ex-type strains are in bold. The newly generated sequences are in red font.
Source publication
Dicephalospora is a genus of Helotiaceae (Helotiales) that presently comprises 14 species, all of which were collected from Asia. The current study describes three additional species and a collection from Chiang Rai and Chiang Mai Provinces, Thailand. The new fungi were identified based on morphological characteristics coupled with phylogenetic ana...
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... phylogram (Figure 1) was generated from maximum likelihood (ML) analysis based on combined LSU and ITS sequence data for the family Helotiaceae. Seventy-nine strains were included in the combined analyses and the dataset comprised 1287 characteristics after alignment (832 characteristics for LSU and 455 characteristics for ITS), including gaps. ...
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... tree topology of the ML analysis is similar to the BI analysis. The best RaxML tree with a final likelihood value of −8372.349462 is presented in Figure As shown in Figure 1, Dicephalospora yunnanica grouped together with strains MFLU 16-0591a and MFLU 16-0591b. This evidence suggests that MFLU 16-0591a and MFLU 16-0591b are conspecific with D. yunnanica. ...
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... GenBank accession information is as follows: MFLU 21-0018: LSU-MZ241826, ITS-MZ241817; MFLU 21-0019: LSU-MZ241827, ITS-MZ241818; MFLU 21-0020: LSU-MZ241828, ITS-MZ241819. Notes: Our collection grouped sister to Dicephalospora rufocornea with 71% ML and 0.70 BYPP support (Figure 1). Furthermore, the pairwise homoplasy index (PHI) showed no significant genetic recombination between D. chiangraiensis and its sister taxa D. rufocornea and D. irregularis (Figure 2). ...
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... GenBank accession information is as follows: MFLU 22-0050: LSU-ON604634, ITS-ON606312; MFLU 22-0053: LSU-ON604635, ITS-ON606313 Notes: Our specimens MFLU 22-0050 and MFLU 22-0053 grouped as a sister clade to Dicephalospora shennongjiana HMAS 279698 and Dicephalospora huangshanica clade ( Figure 1), with 76% ML bootstrap support and 0.94 Bayesian posterior probabilities. Furthermore, the pairwise homoplasy index (PHI) showed no significant genetic recombination between D. inthanonensis and its sister taxa D. huangshanica (Figure 4). ...
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... GenBank accession information is as follows: ITS-ON511117, LSU-ON514038. Notes: Our collection MFLU 22-0054 grouped sister to Dicephalospora rufocornea MFLU 18-1825 and HMAS 279697 (Figure 1). The ITS sequence of our specimen shows 96.7% and 98% similarity to the D. rufocornea (MFLU 18-1825) and D. rufocornea (HMAS 279697), respectively, while the LSU data of our specimen show 99% similarity to D. rufocornea (MFLU 18-1825) across 829 bp. ...
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... Our collection MFLU 22-0054 grouped sister to Dicephalospora rufocornea MFLU 18-1825 and HMAS 279697 (Figure 1). The ITS sequence of our specimen shows 96.7% and 98% similarity to the D. rufocornea (MFLU 18-1825) and D. rufocornea (HMAS 279697), respectively, while the LSU data of our specimen show 99% similarity to D. rufocornea (MFLU 18-1825) across 829 bp. ...
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... specimen, D. irregularis, is morphologically distinct from D. rufocornea by having sessile apothecia, ectal excipulum of textura globulosa to textura angularis, non-amyloid asci, and fusoid-clavate to ellipsoid ascospores, while D. rufocornea is characterized by having stipitate apothecia, ectal excipulum of textura porrecta to prismatica, hymenoscyphus-type apical ring, and fusoid or fusoid-clavate ascospores [2,8]. Dicephalospora irregularis differs from D. sessilis in apothecial shape and branched, filiform paraphyses [2], and our species is more distantly related to D. sessilis than D. rufocornea in the phylogenetic tree (Figures 8-10). Dicephalospora rufocornea (Berk. ...
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... phylogenetic placement of Helotiaceae in our study is similar to that reported by Ekanayaka and coauthors [2]. Eleven species in this genus were grouped with strong 84% ML bootstrap and 0.99 BYPP support (Figure 1). Further, D. chrysotricha grouped with Hymenoscyphus and formed a separate clade with other species of Dicephalospora, with moderate 62% ML bootstrap and 0.95 BYPP support (Figure 1). ...
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... species in this genus were grouped with strong 84% ML bootstrap and 0.99 BYPP support (Figure 1). Further, D. chrysotricha grouped with Hymenoscyphus and formed a separate clade with other species of Dicephalospora, with moderate 62% ML bootstrap and 0.95 BYPP support (Figure 1). The salient discriminatory characters of D. chiangraiensis are smaller asci and smaller ascospores compared to the closely related D. rufocornea. ...
Citations
... We have been conducting comprehensive studies on discomycetes, encompassing investigations into taxonomy, species diversity and evolutionary research, among other aspects (Ekanayaka et al. 2017(Ekanayaka et al. , 2018(Ekanayaka et al. , 2019Lestari et al. , 2023Phutthacharoen et al. 2022). In this study, the authors aim to investigate the diversity of discomycetes in Yunnan Province, China. ...
During the investigations of discomycetes in Yunnan, China, five species of Tatraea were discovered on decayed, decorticated oak trees or unidentified wood. All species have typical disc-like, large fruiting bodies with grey, brown or greyish-green colors. The ITS sequence analysis showed that they belong to Tatraea (Helotiaceae, Helotiales) and the LSU and ITS combination revealed a different topology within the genus. Four species, T. clepsydriformis , T. griseoturcoisina , T. yunnanensis and T. yuxiensis were established as new species, and T. aseptata was collected and described on oak woods. The pairwise homoplasy index (PHI) test results indicated that there is no significant genetic recombination (Φ w = 1.0) between all related species pairs. All the species described here are supported by descriptions, illustrations and multi-gene analyses.
... In Thailand, helotian discomycetes were recorded Ekanayaka et al., 2019;Phutthacharoen et al., 2022), however, there are no records of Rutstroemiaceae members, especially Lambertella. Following the suggestions of Chethana et al. (2021), the current paper employed polyphyletic approaches for identifying Lambertella species, which resulted in ve new species and a geographical record from Thailand. ...
During our excursions for discomycetes from 2019–2021 in forests and plantations in northern Thailand, several Lambertella-like specimens were found. Morphological observation and BLAST sequence data search confirmed that six newly collected specimens belong to Lambertella . Further phylogenetic analysis using maximum likelihood and Bayesian inference analyses based on combined ITS and LSU sequence data and morphological examination coupled with chemical reactions, confirmed six Lambertell a species. Lambertella aurantiaca was established as a new geographical record, and the other five specimens, L. fusoidea, L. phanensis, L. sessilis, L. takensis , and L. tectonae were introduced as novel species. The highlight of the current study is to contribute a complete morphological description of Lambertella aurantiaca since its introduction in 1964, emend the morphological criteria for Lambertella sensu stricto , and provide sequence data for all the Lambertella species described in the current study, including the extant species, L. aurantiaca for the first time, which are essential for future studies.
