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Phylogenetic tree of Cunninghamellaceae constructed using D1-D2 domains of nuc 28S rDNA sequences. Mortierella parvispora was used as an outgroup. Sequences are labeled with their database accession numbers. Support values are from maximum likelihood analyses and Bayesian inference. The sequences obtained in this study are annotated in boldface.
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During a survey of the diversity of Mucorales in Atlantic Forest and upland rainforest soils in northeastern Brazil, two strains belonging to Absidia were isolated. These strains are morphologically and molecularly (nuc rDNA internal transcribed spacer ITS1–5.8–ITS2 and D1–D2 domains of nuc 28S rDNA) distinct from other Absidia species. Absidia cor...
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While surve ying Mucorales diversity in soil from an upland Atlantic forest area in the state of Pernambuco, Brazil, one specimen of Absidia was isolated and characterized based on morphological, physiological, and molecular data (ITS and LSU rDNA regions). The isolate produces well-branched sporangiophores that may end in abortive sporangia that b...
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... Species are usually delineated using the ITS and LSU genetic markers. Some studies also include protein-coding genes such as actin (ACT) and translation elongation factor (EF-1α), which increases the reliability of the phylogenies [9,10] . However, it is well known, that obtaining the ITS rDNA sequence data and protein coding genes in this genus is extremely difficult and often cloning is required to obtain good quality DNA sequences [6,9] . ...
Absidia is one of the most commonly isolated fungi among Cunninghamellaceae. The genus comprises saprobes isolated from soil, dung and other organic debris such as leaf litter. During a survey aimed at exploring the diversity of basal lineages of soil fungi, samples were collected from Nan province, Thailand. This led to the collection of a new Absidia isolate from soil. Characterization of the new isolate was based on morphological characters, colony growth and DNA sequence data. Phylogenetic analyses indicate that the new isolate comprises a lineage distinct from other described species. Morphological characterization showed that the isolate has smaller sporangia and columellae than its sister taxa. Furthermore, physiological data and genetic distance analysis supported the establishment of the new taxon. Hence, in this study, a new species of Absidia (A. zygospora) is introduced based on morphology, phylogeny and physiology.
... et al. and A. macrospora Váňová were reported in China, Czechia, and the USA [25][26][27]. Since 2018, 22 endemic species have been described from Korea, China, Thailand, Australia, USA, Mexico, and Brazil [14][15][16][28][29][30][31][32][33][34][35][36]. Type strains were collected from 17 countries, and the two most investigated countries are China and Brazil, with nine and eight type strains, respectively. ...
... In China, most species of Absidia are recorded in Yunnan, Xinjiang, and Taiwan (Table 3), located in tropical, subtropical, and temperate zones. At the same time, a number of Absidia are described from Brazil and Thailand, which have a similar climate [17,[28][29][30]33,34]. However, species of Absidia are rarely adapted to high temperatures, so that strains in tropical areas are usually described from forest soil or mountains [16,17,28,29,33,34]. ...
... At the same time, a number of Absidia are described from Brazil and Thailand, which have a similar climate [17,[28][29][30]33,34]. However, species of Absidia are rarely adapted to high temperatures, so that strains in tropical areas are usually described from forest soil or mountains [16,17,28,29,33,34]. Consequently, species diversity of Absidia in tropical and subtropical forest soil needs to be further explored. ...
Although species of Absidia are known to be ubiquitous in soil, animal dung, and insect and plant debris, the species diversity of the genus and their ecological habitats have not been sufficiently investigated. In this study, we describe five new species of Absidia from forest and grassland soils in southwestern China, with support provided by phylogenetic, morphological, and physiological evidence. The species diversity and ecological habitat of Absidia are summarized. Currently, 22 species are recorded in China, which mainly occur in soil, especially in tropical and subtropical forests and mountains. An updated key to the species of Absidia in China is also provided herein. This is the first overview of the Absidia ecological habitat.
... [1,2]. Members of Absidia are widely distributed in soils, plant residues, herbivorous dung, decaying substrates and air [2][3][4][5][6][7][8][9][10]. In particular, some species are found in the mycangia of ambrosia beetles (A. ...
Species of Absidia are distributed widely in the environment, while their diversity is insufficiently studied. Three new species, A. frigida, A. gemella and A. longissima, are proposed herein from Xinjiang and Yunnan in China based on phylogenetic, morphological and physiological evidence. According to maximum likelihood (ML), maximum parsimony (MP) and Bayesian inference (BI) analyses, the phylogenetical results suggest that A. frigida, A. gemella and A. longissima are closely related to A. psychrophilia, A. turgida and A. zonata and A. koreana, respectively, based on ITS and LSU rDNA sequences. Absidia frigida is characterized by a lower growth temperature, which does not grow above 24 °C. It differs from A. psychrophilia by sporangiophores, sporangia, columel-lae, collars and projections. Absidia gemella is distinguished from A. turgida by hypha, sporangio-spores, sporangia, projections and sporangiophores. Absidia longissima is discriminated from A. zo-nata and A. koreana by sporangiophores, columellae and collars. The three new species are described and illustrated in this article.
... Species belonging to Absidia are characterized by their simple or branched sporangiophores that commonly arise from stolons with rhizoids never opposed to sporangia (Benny 2005). The deliquescent and apophysate sporangia are subglobose or piriform in shape, and its columellae may or may not have one to three projections on its surface (Benny 2005;Lima et al. 2020). Zygospores are formed within zygosporangia supported by opposed suspensors that have several appendages (Hoffmann et al. 2007). ...
... μm) (Ariyawansa et al. 2015), smaller than the sporangiospores of A. aguabelensis that are globose, subglobose and cylindrical. (Lima et al. 2020)] with one or two projections (rarely three in A. cornuta) on its central part (Lima et al. 2020). However, some projections on the columellae of A. aguabelensis are extremely small that they seem inconspicuous. ...
... μm) (Ariyawansa et al. 2015), smaller than the sporangiospores of A. aguabelensis that are globose, subglobose and cylindrical. (Lima et al. 2020)] with one or two projections (rarely three in A. cornuta) on its central part (Lima et al. 2020). However, some projections on the columellae of A. aguabelensis are extremely small that they seem inconspicuous. ...
During a soil survey for Mucorales in a semiarid region of the state of Pernambuco, Brazil, a specimen of Absidia (URM 8213) was isolated. Both morphological and phylogenetic analyses (ITS and LSU rDNA regions) of this specimen showed that it is a new species. The new species differs from other species in the genus in its ability to concomitantly produce isolated or whorled sporangiophores of up to 8 with fig-shaped and hemispherical columellae, as well as globose, subglobose, and cylindrical sporangiospores. Thus, in this study, we propose a new species of Absidia.
... Sporangiospores spherical, 2.3-3.5 µm diam, 2.8 µm on average. Columellae hemispherical above the Fig. 1 -Phylogenetic tree based on the large ribosomal subunit showing the taxonomic placement of the 16 newly isolated Absidia strains (in bold) compared to previously described species (Lima et al., 2021;Lima et al., 2020;Cordeiro et al., 2020;Crous et al., 2020;Vitale et al., 2012;Walther et al., 2013;Vu et al., 2019;Crous et al., 2018;Zhang et al., 2018;Li et al., 2016;Hoffmann & Voigt, 2009;Ariyawansa et al., 2015;Wanasinghe et al., 2018). Cunninghamella vesiculosa P.C. Misra is used as the outgroup. ...
Absidia healeyae is a new species described in the Mucorales genus Absidia after screening 16 strains of Absidia isolated from seven locations in the state of Victoria in Australia. After initial analysis of the large ribosomal subunit sequence, the genomes of representative strains from two clades were sequenced using short paired-reads. Additional taxonomic markers extracted from the genome sequencing data support the novelty of A. healeyae. The identification of a new species in the genus Absidia, from a relatively small collection of isolates, hints at an unexplored diversity in the early diverging lineages of fungi in Australia.
Novel species of fungi described in this study include those from various countries as follows: Algeria,
Phaeoacremonium adelophialidum from Vitis vinifera. Antarctica, Comoclathris antarctica from soil. Australia,
Coniochaeta salicifolia as endophyte from healthy leaves of Geijera salicifolia, Eremothecium peggii in fruit of Citrus
australis, Microdochium ratticaudae from stem of Sporobolus natalensis, Neocelosporium corymbiae on stems of
Corymbia variegata, Phytophthora kelmanii from rhizosphere soil of Ptilotus pyramidatus, Pseudosydowia backhousiae
on living leaves of Backhousia citriodora, Pseudosydowia indooroopillyensis, Pseudosydowia louisecottisiae
and Pseudosydowia queenslandica on living leaves of Eucalyptus sp. Brazil, Absidia montepascoalis from soil.
Chile, Ilyonectria zarorii from soil under Maytenus boaria. Costa Rica, Colletotrichum filicis from an unidentified
fern. Croatia, Mollisia endogranulata on deteriorated hardwood. Czech Republic, Arcopilus navicularis from tea bag
with fruit tea, Neosetophoma buxi as endophyte from Buxus sempervirens, Xerochrysium bohemicum on surface
of biscuits with chocolate glaze and filled with jam. France, Entoloma cyaneobasale on basic to calcareous soil,
Fusarium aconidiale from Triticum aestivum, Fusarium juglandicola from buds of Juglans regia. Germany, Tetraploa
endophytica as endophyte from Microthlaspi perfoliatum roots. India, Castanediella ambae on leaves of Mangifera
indica, Lactifluus kanadii on soil under Castanopsis sp., Penicillium uttarakhandense from soil. Italy, Penicillium ferraniaense
from compost. Namibia, Bezerromyces gobabebensis on leaves of unidentified succulent, Cladosporium
stipagrostidicola on leaves of Stipagrostis sp., Cymostachys euphorbiae on leaves of Euphorbia sp., Deniquelata
hypolithi from hypolith under a rock, Hysterobrevium walvisbayicola on leaves of unidentified tree, Knufia hypolithi
and Knufia walvisbayicola from hypolith under a rock, Lapidomyces stipagrostidicola on leaves of Stipagrostis sp.,
Nothophaeotheca mirabibensis (incl. Nothophaeotheca gen. nov.) on persistent inflorescence remains of Blepharis
obmitrata, Paramyrothecium salvadorae on twigs of Salvadora persica, Preussia procaviicola on dung of Procavia
sp., Sordaria equicola on zebra dung, Volutella salvadorae on stems of Salvadora persica. Netherlands, Entoloma
ammophilum on sandy soil, Entoloma pseudocruentatum on nutrient poor (acid) soil, Entoloma pudens on
plant debris, amongst grasses. New Zealand, Amorocoelophoma neoregeliae from leaf spots of Neoregelia sp.,
Aquilomyces metrosideri and Septoriella callistemonis from stem discolouration and leaf spots of Metrosideros
sp., Cadophora neoregeliae from leaf spots of Neoregelia sp., Flexuomyces asteliae (incl. Flexuomyces gen. nov.)
and Mollisia asteliae from leaf spots of Astelia chathamica, Ophioceras freycinetiae from leaf spots of Freycinetia banksii, Phaeosphaeria caricis-sectae from leaf spots of Carex secta. Norway, Cuphophyllus flavipesoides on soil
in semi-natural grassland, Entoloma coracis on soil in calcareous Pinus and Tilia forests, Entoloma cyaneolilacinum
on soil semi-natural grasslands, Inocybe norvegica on gravelly soil. Pakistan, Butyriboletus parachinarensis on
soil in association with Quercus baloot. Poland, Hyalodendriella bialowiezensis on debris beneath fallen bark of
Norway spruce Picea abies. Russia, Bolbitius sibiricus on а moss covered rotting trunk of Populus tremula, Crepidotus
wasseri on debris of Populus tremula, Entoloma isborscanum on soil on calcareous grasslands, Entoloma
subcoracis on soil in subalpine grasslands, Hydropus lecythiocystis on rotted wood of Betula pendula, Meruliopsis
faginea on fallen dead branches of Fagus orientalis, Metschnikowia taurica from fruits of Ziziphus jujube, Suillus
praetermissus on soil, Teunia lichenophila as endophyte from Cladonia rangiferina. Slovakia, Hygrocybe fulgens
on mowed grassland, Pleuroflammula pannonica from corticated branches of Quercus sp. South Africa, Acrodontium
burrowsianum on leaves of unidentified Poaceae, Castanediella senegaliae on dead pods of Senegalia
ataxacantha, Cladophialophora behniae on leaves of Behnia sp., Colletotrichum cliviigenum on leaves of Clivia
sp., Diatrype dalbergiae on bark of Dalbergia armata, Falcocladium heteropyxidicola on leaves of Heteropyxis
canescens, Lapidomyces aloidendricola as epiphyte on brown stem of Aloidendron dichotomum, Lasionectria
sansevieriae and Phaeosphaeriopsis sansevieriae on leaves of Sansevieria hyacinthoides, Lylea dalbergiae on
Diatrype dalbergiae on bark of Dalbergia armata, Neochaetothyrina syzygii (incl. Neochaetothyrina gen. nov.) on
leaves of Syzygium chordatum, Nothophaeomoniella ekebergiae (incl. Nothophaeomoniella gen. nov.) on leaves of
Ekebergia pterophylla, Paracymostachys euphorbiae (incl. Paracymostachys gen. nov.) on leaf litter of Euphorbia
ingens, Paramycosphaerella pterocarpi on leaves of Pterocarpus angolensis, Paramycosphaerella syzygii on leaf
litter of Syzygium chordatum, Parateichospora phoenicicola (incl. Parateichospora gen. nov.) on leaves of Phoenix
reclinata, Seiridium syzygii on twigs of Syzygium chordatum, Setophoma syzygii on leaves of Syzygium sp., Starmerella
xylocopis from larval feed of an Afrotropical bee Xylocopa caffra, Teratosphaeria combreti on leaf litter of
Combretum kraussii, Teratosphaericola leucadendri on leaves of Leucadendron sp., Toxicocladosporium pterocarpi
on pods of Pterocarpus angolensis. Spain, Cortinarius bonachei with Quercus ilex in calcareus soils, Cortinarius
brunneovolvatus under Quercus ilex subsp. ballota in calcareous soil, Extremopsis radicicola (incl. Extremopsis
gen. nov.) from root-associated soil in a wet heathland, Russula quintanensis on acidic soils, Tubaria vulcanica on
volcanic lapilii material, Tuber zambonelliae in calcareus soil. Sweden, Elaphomyces borealis on soil under Pinus
sylvestris and Betula pubescens. Tanzania, Curvularia tanzanica on inflorescence of Cyperus aromaticus. Thailand,
Simplicillium niveum on Ophiocordyceps camponoti-leonardi on underside of unidentified dicotyledonous leaf. USA,
Calonectria californiensis on leaves of Umbellularia californica, Exophiala spartinae from surface sterilised roots of
Spartina alterniflora, Neophaeococcomyces oklahomaensis from outside wall of alcohol distillery. Vietnam, Fistulinella
aurantioflava on soil. Morphological and culture characteristics are supported by DNA barcodes.
Absidia is ubiquitous and plays an important role in medicine and biotechnology. In the present study, nine new species were described from China in the genus Absidia , i.e. A. ampullacea, A. brunnea, A. chinensis, A. cinerea, A. digitata, A. oblongispora, A. sympodialis, A. varians , and A. virescens . Besides, two varieties A. cylindrospora var. nigra and A. spinosa var. biappendiculata were elevated to a specific rank as A. nigra comb. nov. and A. biappendiculata comb. nov., respectively. These new taxa were proposed based on a comprehensive investigation of morphological traits (such as shape and size of sporangia, sporangiospores and projections on columellae), physiological feature (maximum growth temperatures), and multi-locus sequences (including internal transcribed spacer, large subunit D1-D2 domains of nuclear ribosomal DNA, partial translation elongation factor 1 alpha gene and actin gene). All species mentioned above are illustrated, and an identification key to all the known species of Absidia in China is included.
Four new species within the genus Absidia, A. globospora, A. medulla, A. turgida, and A. zonata, are proposed based on a combination of morphological traits, physiological features, and molecular evidences. A. globospora is characterized by globose sporangiospores, a 1.0-to 3.5-µm-long papillary projection on columellae, and sympodial sporangiophores. A. medulla is characterized by cylindrical to oval sporangiospores, a 1.0-to 4.5-µm-long bacilliform projection on columellae, and spine-like rhizoids. A. turgida is characterized by variable sporangiospores, up to 9.5-µm-long clavate projections on columellae, and swollen top of the projection and inflated hyphae. A. zonata is characterized by cylindrical to oval sporangiospores, a 2.0-to 3.5-µm-long spinous projection on columellae, and as many as eight whorled sporangiophores. Phylogenetic analyses based on sequences of internal transcribed spacer rDNA and D1-D2 domains of LSU rDNA support the novelty of these four species within the Absidia. All new species are illustrated, and an identification key to all the known species of Absidia in China is included.
