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Phylogenetic placement of known species of the representative yeast species from duckweed (phylum Ascomycota) based on sequences of the D1/D2 region of the LSU rRNA gene. Reference sequences retrieved from the GenBank database are included. The tree was constructed with the maximum-likelihood method and the GTR evolutionary model. Numbers on the branches represent the bootstrap values (>50%) from 1000 random replicates. The scale bar corresponds to a genetic distance of 0.1 substitutions per position. Schizosaccharomyces pombe NRRL Y-12796 T (JQ689077) was used as an outgroup in this analysis. a) A part of the tree showing the phylogenetic relationships of a partial taxa within Debaryomycetaceae, Saccharomycetales incertae sedis, Trichomonascaceae, Phaffomycetaceae, Pichiaceae, Saccharomycodaceae and Saccharomycetaceae b) Part of the tree that shows the phylogenetic relationships of another partial taxa within Metschnikowiaceae, Saccharomycetales incertae sedis and Pichiaceae.
Source publication
The diversity of duckweed (Lemnaceae) associated yeasts was studied using a culture-dependent method. A total of 252 yeast strains were isolated from 53 duckweed samples out of the 72 samples collected from 16 provinces in Thailand. Yeast identification was conducted based on the D1/D2 region of the large subunit (LSU) rRNA gene sequence analysis....
Contexts in source publication
Context 1
... 15 yeast strains showed nucleotide sequence similarities that ranged between 97.11% and 99.41% to their closest species in the GenBank database. Figure 2 (phylum Ascomycota) and Figure 3 (phylum Basidiomycota). The representative sequence data of those yeasts showing the highest similarity of the D1/D2 region of the LSU rRNA gene to the corresponding type strains were submitted to the GenBank database under the following accession numbers: ON065764, ON000832, MZ930476, OP609955, OP555401-OP555438, and OP555998-OP556014. ...
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... results shown in Figures 2 and 3 indicate a slightly higher number of yeast strains in the phylum Ascomycota than in Basidiomycota. However, the most abundant species in this study was Papiliotrema laurentii (55 strains out of 252 strains, which is equivalent to a 21.8% relative frequency). ...
Context 3
... 15 yeast strains showed nucleotide sequence similarities that ranged between 97.11% and 99.41% to their closest species in the GenBank database. Figure 2 (phylum Ascomycota) and Figure 3 (phylum Basidiomycota). The representative sequence data of those yeasts showing the highest similarity of the D1/D2 region of the LSU rRNA gene to the corresponding type strains were submitted to the GenBank database under the following accession numbers: ON065764, ON000832, MZ930476, OP609955, OP555401-OP555438, and OP555998-OP556014. ...
Context 4
... results shown in Figures 2 and 3 indicate a slightly higher number of yeast strains in the phylum Ascomycota than in Basidiomycota. However, the most abundant species in this study was Papiliotrema laurentii (55 strains out of 252 strains, which is equivalent to a 21.8% relative frequency). ...
Citations
... Specifically, duckweed can provide habitats (such as surface of roots and leaves) and resources (such as secretions) for the enrichment of specific microbial assemblages [37,38], e.g., Herbaspirillum, Brevundimonas, ANPR, and Sphingomonas in this study (Fig. 6d), which may facilitate the nutrient removal. Conversely, these specific microbial assemblages may also promote duckweed growth and nutrient recovery via different mechanisms such as nitrogen fixation, phosphate solubilization, production of indole-related compounds and other plant growth-promoting compounds [39][40][41]. Thus, this study demonstrates that combined treatment with pre-aeration and carrier addition more effectively strengthens the purification performance of a deep DWP than single treatment, elucidates the roles of duckweed and microorganisms in deep DWPs under combined treatment, and provides new insights into the improvement and application of DWPs. ...
... Elution was performed at 40 °C with 0.3 mL/min flow rate. The compound was detected with a UV detector at 280 nm and compared to the standard IAA (Sigma, USA) as described by Kajadpai et al. (2023). Siderophore production was tested on chrome azurol S (CAS) agar (Schwyn and Neilands 1987) which overlaid with 0.5X TSA to provide a suitable medium for growth of fastidious bacteria. ...
Duckweed has been highlighted as an invaluable resource because of its abilities to remove nitrogen and phosphorus from wastewater coupling with the production of high starch/protein-containing plant biomass. Duckweed recruits microbes and particularly forms a stable “core” bacterial microbiota, which greatly reduces the colonization efficiency of plant growth-promoting bacteria (PGPB). In this study, natural duckweeds were enriched in a sterilized-partially treated wastewater effluent from a poultry farm. After 24 days of cultivation, the duckweed-associated bacteria (DAB) were isolated and evaluated for their plant growth-promoting (PGP) potentials by co-cultivation with axenic Spirodela polyrhiza. Ten species were found in more than one location and could be considered candidates for the stable “core” DAB. Among them, all isolates of Acinetobacter soli, Acidovorax kalamii, Brevundimonas vesicularis, Pseudomonas toyotomiensis, and Shinella curvata increased duckweed growth in Hoagland medium. The highest PGP ability was observed in Sh. curvata W12-8 (with EPG value of 208.72%), followed by Paracoccus marcusii W7-16 (171.31%), Novosphingobium subterraneum W5-13 (156.96%), and Ac. kalamii W7-18 (156.96%). However, the highest growth promotion in the wastewater was observed when co-cultured with W7-16, which was able to increase biomass dry weight and root length of duckweed by 3.17 and 2.26 folds, respectively.
Strains of the ascomycetous yeast Metschnikowia pulcherrima isolated from the internal tissues of fruits were evaluated for phytohormonal activity. IAA production in the culture liquid was determined by the chromatographic method (HPLC-UV). The production of the phytohormones zeatin and gibberellin (GA3) in the culture liquid was determined by a chromatographic method using a quadrupole time-of-flight mass spectrometry detector (Q- TOF LC / MS). IAA and zeatin were detected in the culture liquid of all strains studied. Synthesis of gibberellin (GA3) was detected in only 5 of 18 strains examined. On average, the strains showed the highest activity in the synthesis of IAA (6.668 µg/mL and 2.135 mg/g). Zeatin content was 3 orders of magnitude lower (0.365 ng/mL and 0.129 µg/g) and GA3 content was 4 orders of magnitude lower (0.076 ng/mL and 0.027 µg/g). According to our results, the yeast M. pulcherrima is not an active producer of zeatin and GA3 in amounts relevant to the plant. On the other hand, the average level of IAA synthesis detected in M. pulcherrima strains in this study is within the range of concentrations that can stimulate plant development and growth. Thus, endophytic strains of M. pulcherrima that produce plant-relevant levels of IAA could be improved to increase their synthesis ability and used as plant growth promoters in agricultural crops.
