Phylogenetic analysis of legume enod40 genes and putative ENOD40 peptides. A, Distance tree of legume enod40 cDNA sequences deduced from the alignment of cDNA sequences starting 20 nucleotides 5 to region I. The alignment was performed using CLUSTAL V. The scale represents the distance between sequences. B, Multiple alignment of region I encoded putative oligopeptides from legumes and nonlegumes. The conserved amino acids are boxed. The conserved nucleotides present in all legume and nonlegume enod40 transcripts are presented below. Plant species and GenBank database accession numbers used in alignments are as follows: Tr, white clover (1, AF426838; 2, AF426831; and 3, AF426840, this work), Lj, L. japonicus (1, AJ271787; 2, AJ271788); Sr, Sesbania rostrata (Y12714); Gm, soybean (1, D13503; 2, D13504); Pv, bean (X86441); Ms, alfalfa (X80263); Mt, Medicago truncatula (X80264); Vs, vetch (Vicia sativa; X83683); Ps, pea (X81064); Nt, tobacco (X98716); and Os, rice (ABO24054).