Fig 9 - available from: Journal of Materials Science: Materials in Medicine
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Photomicrograph showing osteopontin staining for woven bone formation through the entire thickness of the onlay DSM/SCPC/hBMSC sheet graft. The onset demonstrates direct bone formation on the surface and in between the SCPC particles. The dark staining of the cells indicates differentiated osteoblasts
Source publication
Tissue regeneration and neovascularisation in cases of major bone loss is a challenge in maxillofacial surgery. The hypothesis of the present study is that the addition of resorbable bioactive ceramic Silica Calcium Phosphate Cement (SCPC) to Declluraized Muscle Scaffold (DSM) can expedite bone formation and maturation. Two surgical defect models w...
Context in source publication
Context 1
... staining confirmed the formation of bone matrix within the defect as well as through the entire thickness of the grafted onlay (Fig. 9). We used a colour deconvolution algor DMSC ithm (Aperio Technologies, Inc.) to quantify the osteopontin immunopositive reaction. The intensity of osteopontin immune staining increased in the following order: centre of the defect > interface between new and host bone > newly regenerated bone. Statistical significance was noted with this ...
Citations
... Previously, we evaluated the use of decellularised skeletal muscles( DSM) seeded with mesenchymal stem cells (MSCs) and bioactive glass SCPC and found that this combination can enhance and stimulate bone regrowth and neovascularization [12,13]. It was concluded that addition of biomaterials augments the effects of DSM environment; thus, the use of silica calcium phosphate composite (SCPC) may promote osteoinduction in the seeded exogenous cells and result in recruitment of additional boneforming cells to the area. ...
... There has been considerable research performed in the literature on the use of DSM for bone augmentation [12,13]. When DSM is implanted in living tissues, it creates an extracellular matrix that permits constant communication between the cells and the extracellular framework. ...
... The study showed the role of osteoblasts rather than osteoclasts in the resorption of SCPC bone graft material [21]. In other hand, the role of DSM in neovascularization and angiogenesis at the site of bone regeneration was well documented [12]. This may indicated that vascularity at the site of the bone graft does not cause resorption of the graft, conversely it is crucial for bone regeneration. ...
Bone augmentation is a vital area of research because of its high clinical demand and the reported complications associated with the available biomaterials.
Purpose: The study assess the role of decellurized skeletal muscle (DSM) when combined with synthesized porous bioactive silicon carbide (SiC) ceramic and evaluated its ability to augment calvarious bone at rat`s model. Material&
Methods: Eighteen rats were divided into two groups; Group 1 (n=9), SiC discs (10 mm x 0.2 mm) treated with 20% NaOH were placed as an onlay grafts on calvarial bone. Meanwhile, in Group 2 (n=9), SiC discs (10 mm x 0.2 mm)were covered with DSM. After 12 weeks, the grafted tissues were harvested and examined using cone-beam computed tomography (CBCT), mechanical testing, and histological analysis.
Results: CBCT showed more radio-opacity for the remnant of SiC compared to native bone was noted in group2 at surface area and volume o at 2.48mm²+/- (Sd=1.6) and 14.9+/-(Sd=7.8 )mm3 respectively. The estimated quantitative average surface area of the radio-opacity for group 1 and volume were 2.55 mm²+/- (Sd=3.7) and 11.25+/-(Sd=8.9), respectively. Mechanically, comparable values of the flexural strength and statistically significant higher modulus of elasticity of calvaria in Group 1 compared to Group 2 and control (P < 0.001). Histologically, group2 region of woven bone was seen close to the lamellar bone (native bone), and there was immature bone present near the implanted SiC.
Conclusion: The tested construct made of SiC/DSM has potential to osteointegrate into native bone, making it a suitable material for bone augmentation.
Background
Bone augmentation is a vital area of research because of its high clinical demand and the reported complications associated with the available biomaterials. Purpose: The study assess the role of decellurized skeletal muscle (DSM) when combined with synthesized porous bioactive silicon carbide (SiC) ceramic and evaluated its ability to augment bone calvaria in a rat model.
Material and Methods
Eighteen rats were divided into 2 groups; group 1 (n=9), SiC discs (10 × 0.2 mm) pre-treated with 20% NaOH were placed as an onlay grafts on calvarial bone. Meanwhile, in group 2 (n=9), SiC discs pre-treated with 20% NaOH (10 × 0.2 mm) were covered with DSM. After 12 weeks, the grafted tissues were harvested and examined using cone-beam computed tomography, mechanical testing, and histologic analysis.
Results
Cone-beam computed tomography for group 2 showed more radio-opacity for the remnant of SiC compared with native bone. The surface area and volume of radio-opacity were 2.48 mm ² ± 1.6 and 14.9 ± 7.8 mm ³ , respectively. The estimated quantitative average surface area of the radio-opacity for group 1 and volume were 2.55 mm ² ± (Sd=3.7) and 11.25 ± (Sd=8.9), respectively. Mechanically, comparable values of the flexural strength and statistically significant higher modulus of elasticity of calvaria in group 1 compared with group 2 and control ( P <0.001). Histologically, group 2 region of woven bone was seen close to the lamellar bone (native bone), and there was immature bone present near the implanted SiC.
Conclusion
The tested construct made of SiC/DSM has potential to osteointegrate into native bone, making it a suitable material for bone augmentation.
