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Silver stainable nucleolar organizer regions (AgNORs) are replicatory markers which may have a place in objectively characterizing dysplasia.
A study of various morphometric parameters related to AgNORs was performed in basal and parabasal layers of normal human oral epithelium, nondysplastic leukoplakia, and dysplastic leukoplakia employing photom...
Citations
... Several reports have suggested that variations in AgNOR counts may reflect the state of activation or degree of malignancy of the lesion involved [16]. The AgNOR count is the most appropriate marker to differentiate between dysplastic and nondysplastic leukoplakia [17]. AgNOR analysis may be useful as a quantitative marker of incipient cellular alterations and hence would be helpful in assessing suspicious lesions and thus can be regarded as a valuable adjunct [18]. ...
Background
Silver stainable nucleolar organizer regions (AgNORs) have proven to exhibit utmost importance due to their higher occurrence in the nucleus especially in malignant cells than in normal. Thus, they assist in the examination of nucleolar structures and variations in nucleolar activity.
Aim
Quantitative and qualitative analysis in relation to the number and area of AgNOR in tissue sections of the normal oral mucosa (NOM), oral leukoplakia (OL), and oral squamous cell carcinoma (OSCC) was the main aim of the study.
Materials & method
A total of 50 cases comprising 20 OL with dysplasia, 20 OSCC cases, and 10 samples of normal oral mucosa were taken. Silver nitrate (Sol A) & gelatin (Sol B) solutions were freshly prepared for staining the lesional slides.
Results
The mean value of nuclear profile area (A Nuc) was comparatively higher in oral leukoplakia i.e. 41.97 and in oral squamous cell carcinoma i.e. 62.36 in comparison to the control group where it was 36.19. The mean value of a single AgNOR profile area per nucleus (A NOR) was found to be comparatively lower in both study groups i.e. oral leukoplakia (2.76) and oral squamous cell carcinoma (1.61) in comparison to the control group (3.45) . The mean value of total AgNOR profile area per nucleus (TA NOR) and the number of profiles of AgNORs per nucleus (n NOR) were found higher in both study groups (oral leukoplakia and oral squamous cell carcinoma) as compared to normal oral mucosa of the control group.
However, the findings of all four parameters of morphometric analysis were found to be significantly associated with disorder of oral mucosa i.e. cases of oral leukoplakia and oral squamous cell carcinoma (P value <0.01).
Conclusion
It can thus be suggested that the mean AgNOR count displayed a higher value in OSCC. Hence, the number of AgNORs in nuclei increases as epithelial cells undergo malignant transformation which is designated that mean AgNOR count may contribute to establishing the prognosis of a lesion.
... On the other hand, Spolidorio et al. [19] found no difference in the AgNORs count between the ED and OSCC; however, a distinction was found in the AgNORs morphology based on their size, shape, and distribution pattern within the nucleus. Although various mean AgNOR related parameters (count, area, perimeter, and proportion) can be used to compare normal oral epithelium from dysplastic and nondysplastic leukoplakia [24], according to Garg et al. [27], the AgNOR count is the most appropriate marker to differentiate between dysplastic and nondysplastic leukoplakia. ...
Histologically, these lesions can range from hyperkeratosis and acanthosis to epithelial dysplasia and even OSCC. The aim of this study was to investigate the proliferative activity, using AgNORs quantification proteins, in low- and high-risk oral epithelial dysplasia, OSCC, and nondysplastic epithelium (inflammatory fibrous hyperplasia). The sample was divided into 4 groups: G1: 10 cases of inflammatory fibrous hyperplasia (IFH), G2: 11 cases of low-risk epithelial dysplasia (LD), G3: 10 cases of high-risk
epithelial dysplasia (HD), and G4: 11 cases of OSCC.The quantitative analysis was performed using an image processing software in photomicrographs at 1000x magnification. The one-way ANOVA was used for comparison of the mean AgNORs counts between the study groups. The mean AgNORs count was significantly higher (𝑃 ≤ 0.01) in OSCC when compared to IFH and the LD; however, it was not statistically different from HD. The mean number of LD was significantly lower than the HD and OSCC, with
no difference related to IFH. AgNORs quantification can be an important and cheap method to help in the determination of the degree of epithelial dysplasia and, consequently, in the analysis of their potential for malignant transformation.
Our current study is done to explore the possible mechanisms to elaborate on the growth inhibitory effect of baicalein (BE) in human lung carcinoma. Initially, BE (25 and 50 µM) treatment for 24 h, suppressed the viability and inhibited population growth in A549 cells. BE upholds the production of reactive oxygen species (ROS) with concomitant replenishment of glutathione, catalase, and glutathione peroxidase activity. The expression level of nuclear factor erythroid 2‐related factor 2 and heme oxygenase‐1 markedly increased after BE treatment will intimidate A549 cells proliferation by the ROS‐independent pathway via the antioxidant pathway. In vivo investigations were carried out on BE (12 mg/kg, oral) in benzo(a)pyrene (B(a)P; 50 mg/kg, oral) induced lung carcinogenesis in mice. BE induces caspase‐dependent apoptosis by increasing the levels of cytosolic cytochrome c accompanied by upregulating the outflow of p53, Bax, and caspase‐3 with a concomitant abatement in the outflow of Bcl‐2 in both in vitro and in vivo. In the murine model, BE treatment hindered the countenance of proliferation‐related proteins (argyrophilic nucleolar organizing regions and proliferating cell nuclear antigen). Additionally, appraisal of the cell nucleus by transmission electron microscopic assessment uncovered that BE treatment adequately counteracts B(a)P‐induced lung cancer cell survival. During the transition of the G0/G1 phase, BE is arrested in the cell cycle process. This might be the cause of a substantial increase in the appearance of p21Cip1 with concomitant downregulating the expressions of CDK4, cyclin D, and cyclin E both in vitro and in vivo. Our results conclude that BE treatment induced apoptosis and repressed proliferation both in vitro and in vivo of human lung carcinoma.
The periodontal lesions with cellular proliferation can be assessed by various methods. One of the methods to determine the proliferative activity is silver-staining argyrophilic nucleolar organizer region (AgNOR) staining. AgNOR can be used as diagnostic and prognostic tool for many of periodontal lesions which are proliferative in nature and may be quantitative marker of incipient cellular alterations before the histologic hallmarks appear. Hence, this paper reviewed about the periodontal applications of AgNOR staining.
Squamous cell carcinoma arising from oral mucosal epithelium remains a lethal and deforming disease due to tumour invasion, oro‐facial destruction, cervical lymph node metastasis and ultimate blood‐borne dissemination. Worldwide, 300,000 new cases are seen each year, with a recent and significant rise in incidence affecting particularly the young. In order to rationalize perspectives on preventive strategies in oral cancer management, this paper addresses a number of fundamental questions regarding carcinogenesis: Proliferation ‐ what epithelial cell changes precede tumour development? Position – why are certain oral sites so predisposed to cancer? Progression ‐ why do some precursor lesions progress to invasive carcinoma and others do not? Prediction ‐ how can we predict individual patient and/or lesion behaviour to prevent disease progression? By improving our understanding of oral carcinogenesis, can we thereby facilitate more effective primary, secondary and tertiary preventive strategies and ultimately reduce the global burden of OSCC?
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Curcumin has been reported to suppress different types of clinical and experimentally-induced tumors, but due to less absorption and quick metabolism it show poor bioavailability. The present study was envisaged to investigate the possible synergistic effect of combined treatment of curcumin with piperine in suppression of diethylnitrosamine (DENA)-induced hepatocellular carcinoma (HCC) in rats, owing to permeability enhancing effect of latter. HCC was induced by supplying DENA (0.01%) in drinking water for 10 weeks. The rats were treated with curcumin (100mg/kg; p.o.) per se and curcumin along with piperine (20mg/kg; p.o.) for 4 weeks post HCC induction. The combined treatment significantly attenuated the morphological, histopathological, biochemical, apoptotic and proliferative changes in the liver and serum in comparison to curcumin per se and vehicle control group. The results of present study concluded that curcumin in combination with piperine shows better suppression of DENA-induced HCC in contrast to curcumin per se.
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