Figure 1 - uploaded by Kavitha Raja
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Photomicrograph represents the transverse section of spine stained by Hematoxylin and Eosin. Arrow indicates the venom secretory cells
Source publication
Catfishes, come under the order Siluriformes, they represent as venomous fishes having venom glands in that dorsal and pectoral spines that are speared into place when threatened. The catfish, Tachysurus dussumieri spine venom is unsafe and having neuromuscular blocking activity, envenoms causes instant, local and intense pain, and a variable exten...
Context in source publication
Citations
... These peptides have been isolated through anchovy sauce [10], sea hare [11], sea slug [12], tuna dark muscle [13], squid [14], shrimp shell [15], cod, plaice, salmon [16], Nemipterus japonicas backbone [17]. In our previous studies, the crude extracts from byproducts of marine catfish Tachysurus dussumieri exhibited antiproliferative activity in HT 29 cells [18][19][20]. Therefore, the present study was to investigate the chemotherapeutic and anticancer properties of purified protein from the epidermal mucus of marine catfish Tachysurus dussumieri on the human colon adenocarcinoma cell line. ...
The present study aimed to investigate the purified protein from the epidermal mucus of marine catfish Tachysurus dussumieri on the human colon cancer cell line. The bioactive protein was purified with the Anion exchange chromatography and the collected fractions were then tested to assess cell viability in HT 29 cells through the MTT assay. The most responding active purified protein fraction (PPF III) was characterized with the MALDI-TOF/MS it shared a similar homology and sequence with 90% of antimicrobial peptides from external secretions of amphibians. Typical morphological changes of apoptotic cells, including cell shrinkage and detachment, DNA damage, and nuclear condensation were observed after the treatment of bioactive protein. PPF III triggered ROS, increasing the LDH activity, disruption of mitochondrial membrane potential, and upregulation of Cleaved caspase 3/9, Cytochrome-c, Bax, and downregulation of Bcl-2 protein and gene expression on HT 29 cells.
Graphical Abstract
