Figure 3 - uploaded by Soheir Ghonemy
Content may be subject to copyright.
Photomicrograph of normal skin (control group) is showing osteopontin expression in the basal layer of the epidermis (osteopontin immunostain, ×200)
Source publication
Background:
Osteopontin (OPN) has been postulated to have a role in several T-helper (Th) 1 and Th 17-mediated diseases including psoriasis (PS), through multiple mechanisms sharing in the onset and worsening of PS, OPN shares in induction of keratinocyte proliferation through inhibiting keratinocyte apoptosis, OPN acts as a proinflammatory agent...
Citations
... In addition, fibroblasts and keratinocytes may be influenced by osteopontin through matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), contributing to abnormal tissue remodeling observed in psoriatic skin [16,17]. Finally, sOPN promotes enhanced epidermal proliferation via keratinocyte apoptosis inhibition and is also associated with vascular changes leading to increased angiogenesis in psoriatic lesions [18]. ...
... In all 16 evaluated articles, there was a higher expression of OPN in the psoriasis (PsO) group than in the healthy control (HC) groups. Twelve groups measured serum OPN [17,[19][20][21][22][23][24][25][26][27][28][29], four reports investigated tissue OPN [7,18,23,30], and the expression of OPN genotypes and mRNA in peripheral blood mononuclear cells was evaluated in three studies [24,29,31]. The correlation between serum and tissue OPN was investigated in one paper and no correlation was found [23]. ...
... Although OPN concentration is known to be elevated in psoriatic plaques, it may be surprising to learn that this is also the case in non-lesional skin in PsO patients. Such OPN overexpression in seemingly healthy skin may explain Koebner phenomenon and the constant readiness of the skin to promote plaque formation [18,23,31]. The OPN distribution in psoriatic skin differs from that noted in healthy controls. ...
Psoriasis is a chronic systemic disease with an immunological basis and a complex pathophysiology. The chronic inflammatory status of psoriasis is associated with several comorbidities, such as metabolic syndrome, obesity, and cardiovascular disease. The development of psoriasis is influenced by osteopontin, a glycoprotein that influences physiological and pathological reactions by modulating Th1 and Th17 cellular responses, stimulating keratinocyte proliferation, regulating cellular apoptosis, and promoting angiogenesis. The recent identification of immune pathways involved in psoriasis development has facilitated the development of biological treatments; however, a better understanding of the intricate relationship between underlying inflammatory processes, psoriasis development, and accompanying comorbidities is needed for improved disease management.
... OPN was expressed in skin biopsies from psoriasis lesions and peripheral blood mononuclear cells [75]. OPN may play a role in pathogenesis of psoriasis as suggested on 532 the findings of previously reported case-control studies [19][20][21][22][23][24][25][26][27][28][29][30]. ...
... To my knowledge, this is the 3 rd study that was performed in Iraq which reported a higher serum OPN in psoriasis compared to controls [39,84]. Global previous studies indicated an increase in plasma/serum levels in individuals with psoriasis [20][21][22][23][24][25][26][27][28][29][30]. However, the reported studies (A case-control studies with one cross-sectional, one systematic review and meta-analysis) were performed on variable study population sizes which range from 12 to 117 psoriatic patients (Supplementary file). ...
... It was demonstrated that OPN expression in the skin of patients is elevated but its expression level is also associated with the severity of disease. 25,26 In addition, the role of OPN variants on psoriasis susceptibility was evaluated. Abdel-Hay et al 27 analyzed rs4754 C>T and rs9138 A>C, but showed no significant differences in the genotypes frequency between patients and controls. ...
Purpose:
Atopic dermatitis (AD) is a chronic, relapsing inflammatory disease, caused by environmental and genetic factors, which lead to immunological abnormalities. Osteopontin (OPN), also named secreted phosphoprotein 1 (SPP1), is a protein involved in the pathogenesis of numerous autoimmune and inflammatory conditions. However, its role in AD has not been fully elucidated. Therefore, we aim to gain an insight into the role of OPN in AD pathogenesis through investigating its gene single nucleotide polymorphisms (SNPs) and their possible associations with disease clinical features.
Patients and methods:
A total of 182 Caucasian participants (45 AD patients and 137 gender- and age-matched controls) were studied. Genomic DNA was isolated from peripheral blood samples. Genotyping for the rs1126616 C>T, rs1126772 A>G, rs9138 A>C, and rs3841116 T>G SNPs was performed by real time polymerase chain reaction (RT-PCR).
Results:
The frequency of the minor TT genotype and the T allele of rs1126616 C>T was higher in AD patients compared to controls (P = 0.019, OD = 4.86, 95% CI = 1.46-16.20, and P = 0.047, OR = 1.77, 95% CI = 1.04-3.00, respectively) and was associated with the higher prevalence of asthma (P = 0.017, OR = 3.73, 95% CI = 0.71-19.67, and P = 0.004, OR = 3.96, 95% CI = 1.53-10.25, respectively). Likewise, the minor GG genotype and the G allele of rs1126772 A>G were more frequent in AD patients (P = 0.026, OR = 3.27, 95% CI = 1.29-8.33, and P = 0.013, OR = 1.94, 95% CI = 1.18-3.21, respectively) and were associated with the increased incidence of asthma (P = 0.016, OR = 5.06, 95% CI = 1.14-22.49, and P = 0.002, OR = 4.40, 95% CI = 1.71-11.35, respectively). Furthermore, haplotype frequency estimation determined the four-loci haplotype TGCT, as a significant risk factor for AD compared to controls (P = 0.031, OR = 9.48, 95% CI = 1.23-71.91).
Conclusion:
Our results suggest that the variation in the OPN gene might be associated with AD and increased incidence of asthma in Caucasians. Further studies should be conducted to look into the possible role of OPN as a biomarker for AD.
... Osteopontin is involved in the pathophysiology of psoriasis by different mechanisms through its expression by lesional keratinocytes, inflammatory cells, and endothelial cells (Li et al. 2018, Abdel-Mawla et al. 2016. ...
The study aimed to contribute to understanding the role of CRP, chemerin, fetuin-A and osteopontin and to assess their suitability as biomarkers of early stages of cardiovascular diseases in psoriasis vulgaris. Serum levels measured in 28 patients and 22 controls. Patients: increased levels of CRP (p<0.001), chemerin (p<0.05), osteopontin (p<0.05) and decreased levels of fetuin-A (p<0.05), significant relationships between CRP and fetuin-A (rho=0.530, p<0.01), CRP and chemerin (rho=0.543, p<0.01), CRP and age (rho=0.590, p<0.001), osteopontin and fetuin-A (r=-0.415, p<0.05), chemerin and PASI score (rho=-0.424, p<0.05). We confirmed specific roles of the biomarkers in psoriasis. CRP, fetuin-A and osteopontin could be considered appropriate markers for the detection of early stages of cardiovascular diseases.
... 20 In our study, a significantly higher concentration of OPN in the group of psoriatic patients in comparison to the healthy volunteers (p < 0.001) was observed, which is in agreement with the results reported by other authors. [21][22][23][24] In 2016, Abdel-Mawla et al. 25 observed significantly higher OPN expression in the psoriatic plaques in comparison to the healthy skin as well as to the unchanged skin in psoriatic patients. Moreover, the expression of OPN in the unchanged skin of psoriatic patients was significantly higher than in the healthy skin of the healthy volunteers. ...
... The authors also showed a positive correlation of OPN expression with the density of inflammatory infiltration in the skin. 25 This study confirms the participation of OPN in the local inflammatory process occurring in psoriatic lesions. It seems that an increased concentration of OPN in the unchanged skin of psoriatic patients may stimulate chemotaxis of inflammatory cells towards the predisposed site and contribute to the development of psoriatic plaque. ...
... However, different data was presented by Kadry et al., 22 who showed a positive correlation between plasma OPN concentration and the clinical severity of psoriasis. Abdel-Mawla et al., 25 using immunohistochemistry, also observed a positive relationship between the OPN expression in the affected psoriatic skin and PASI. ...
Background:
Psoriasis is a chronic, autoinflammatory disease characterized by activation and differentiation of naive T lymphocytes towards T helper CD4+ (including Th1 and Th17) and T cytotoxic CD8+. Osteopontin (OPN), which plays an important role in both physiological processes and inflammatory, neoplastic and autoimmune diseases, is also considered in the context of psoriasis pathogenesis. Current data indicates that OPN is a multifunctional protein involved in the modulation of Th1 and Th17 cellular responses, in stimulating keratinocyte proliferation, and in the regulation of cellular apoptosis.
Objectives:
The assessment of OPN and interleukin 17 (IL-17) concentrations in the peripheral blood of psoriatic patients in comparison to healthy volunteers as well as the correlations of OPN and IL-17 with the severity of psoriasis.
Material and methods:
The study included 107 male psoriatic patients and 41 age-matched healthy men. The serum concentrations of IL-17 and OPN were examined using the enzyme-linked immunosorbent assay (ELISA) method. The skin change severity of psoriasis was assessed using the Psoriasis Area and Severity Index (PASI), Body Surface Area (BSA), Physician Global Assessment (PGA), and Dermatology Life Quality Index (DLQI).
Results:
Psoriatic patients had significantly higher concentrations of OPN (31.65 ng/mL on average) than the healthy volunteers (11.42 ng/mL on average) (p < 0.001). Interleukin 17 was also higher in psoriatic patients (0.53 pg/mL on average) compared to healthy volunteers (0.09 pg/mL on average) (p < 0.001). There was no significant correlation between OPN and IL-17 concentrations in psoriatic patients and in healthy volunteers. Psoriasis severity correlated positively to IL-17 serum concentration, but not to OPN.
Conclusions:
Although the study did not show a relationship between OPN and IL-17 concentrations in psoriatic patients, it should be emphasized that serum concentrations were significantly higher in the patients with psoriasis compared to healthy volunteers.
... Abdel-Mawla et al. [25] had a case-control study on 18 (eight men and 10 women) patients of chronic plaque psoriasis; their ages ranged between 12 and 64 years, and a control group of 18 apparently healthy volunteers matched for sex and age. Two skin biopsies were taken from psoriatic patients. ...
... OPN is a multifunctional protein that plays an important role in the inflammatory processes inhibiting Th2 response and promoting Th1 cytokine function, with a crucial role in leukocyte migration [25]. It is secreted in autoimmune diseases such as lupus erythematosus, and influences inflammation of immediate and delayed-type allergies and granuloma formation. ...
... A statistically significant difference was found between psoriatic patients and controls regarding OPN expression in the epidermal skin layers between lesional and nonlesional skin groups, nonlesional and control groups, and finally, lesional skin and control groups. These results possibly indicate that in some psoriatic patients, nonlesional skin might be predisposed to develop the disease, as it can occur in the so-called Koebner phenomenon may be due to elevated OPN expression [25]. ...
