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Phenylpropanoid biosynthesis and Coumarin biosynthesis Pathway in G. littoralis. The red color small rectangle indicates that metabolite content is significantly upregulated, the green small rectangle indicates that metabolite content is significantly down regulated. The blue character and formula represent phenylpropanoid biosynthesis. The green character and formula represent Coumarin biosynthesis. The dotted line part is a speculated coumarin metabolic pathway. (Color figure online)
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The dried root of Glehnia littoralis is a traditional Chinese herbal medicine mainly used to treat lung diseases and plays an important role in fighting coronavirus disease 2019 pneumonia in China. This study focused on the key enzyme gene GlPS1 for furanocoumarin synthesis in G. littoralis. In the 35S:GlPS1 transgenic Arabidopsis stud...
Citations
... The U6/8DT enzymes in this chain [38][39][40][41][42], as well as psoralen and angelicin synthases [43][44][45][46][47], have been well studied. Only one marmesin synthase was found, and not in the Apiaceae family but in Ficus carica, and its homologues have not yet been found in Apiaceae [48]. ...
... Among these furanocoumarins, three compounds, namely, psoralen, imperatorin, and isoimperatorin, were accumulated when transgenic plant was given a salt stress. From this finding, it is also suggested that the adequate stress has significantly increased the economic benefits for enhancing G. littoralis quality (Ren et al. 2023). ...
Medicinal and aromatic plants (MAPs) are the reservoirs of numerous life-saving drugs called secondary metabolites including terpenoids, essential oil, steroids, saponins, alkaloids, phenolics, etc. These secondary metabolites are the group of a variety of chemical compounds produced by the plant cell in different metabolic pathways that branch off from primary metabolic pathways. The quality and quantity of secondary metabolite in MAP are completely dependent on environmental conditions; moreover, the commercial production of secondary metabolites is also dependent on the area of cultivation of MAPs. The systematic secondary metabolite production can be enhanced through biotechnological intervention with minimal downstream processing. The tissue culture and transgenic technologies available in the current era of agriculture science have been advocated as effective tools for increasing the synthesis of these metabolites at an industrial scale. This chapter focuses on the recent advances made in the production of various secondary metabolites by deploying tissue culture and transgenic technologies.KeywordsSecondary metaboliteTransgenicHairy rootMedicinal plantBiotechnologyTissue culture
To fully understand the vast biological complexity that exists within cells, omics studies must be conducted. And the data obtained from these omics platforms needs to be validated in order to reach the final conclusion. This multi-omics data largely exhibits the data generated from genome, transcriptome, proteome, metabolome, and epigenome. Integration of multi-omics approaches appears to be more promising in understanding complicated biology systematically and comprehensively. The production of vast amounts of omics data and the development of computational bioinformatics have made it much easier to integrate these two potent tools to elucidate the functional application for recognizing the molecular interactions taking place within the cell. Multi-omics approach leads to the finding of the associated biological network pathways and the genes responsible for the production of natural products within plants. With the advancement of the high-end instrumentation like sequencing technology, mass spectrometry, and controlled phenotyping facility, it is more informative to gain the proper function of the gene and its interaction with the environmental signals. In this chapter, a brief overview on broad omics approaches and the ways for associating the multi-omics data to draw out the functions of various genes involved in the production of natural compounds within the plants are mentioned.
Обзор посвящен фуранокумаринам – классу веществ, представляющих собой совмещение пиронового, бензольного и фуранового колец, обладающих системой сопряженных двойных связей (которая в ряде случаев может нарушаться). Эта группа соединений широко изучается в настоящее время благодаря своим фототоксическим и лекарственным свойствам. В работе рассмотрены фуранокумарины естественного происхождения, выявленные в семействе Umbelliferae (Зонтичные), или Apiaceae (Сельдерейные), структурировано их многообразие, описана история исследования, известные к настоящему времени этапы их биосинтеза, а также некоторые примеры их биологической активности в растении, в культуре клеток и при медицинском использовании.
