Figure 1 - uploaded by Saurabh Chawla
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Perianal tape test detecting Pinworm eggs: The sticky side of cellophane tape was attached to the perianal region mice and then it was pasted on microscopic slide. The slide was examined under microscope with 10X magnification and these eggs were observed.
Source publication
Syphacia obvelata infection was diagnosed in mice during routine health monitoring of rodent colony at Animal Facility, ILS-NISER, Bhubaneswar. Entire mice colony was treated with fenbendazole mixed in drinking water @70 mg/liter in combination with topical spray of injectable 0.1% ivermectin solution to arrest the disease. At the same time mice we...
Citations
... Parasitic infection occurs through the fecal-oral route (Effler et al., 2008). Although pinworms are generally nonpathogenic (Abdel-Gaber et al., 2018), and the resulting clinical signs are rare unless there are heavy loads of infection in immunocompetent animals (Chawla et al., 2015), pinworms may have deleterious effects on several research implications (Agersborg et al., 2001). ...
During screening laboratory mice (Mus musculus) at the Zoology Department of the College of Science, King Saud University, some tiny oxyurid nematodes were detected from the large intestine of these rodents. Worms were identified using morphological and morphometric description. Furthermore, DNA was extracted from worms and subjected to polymerase chain reaction to amplify 18S rDNA and ITS (ITS1, 5.8S and ITS2) regions. Worms detected from caecum and colon of mice were detected in 80% of mice investigated. Morphologically, worms showed elongated body with tapered anterior end and narrow posterior part with cuticular annulations. Male worms measured 0.71-1.12 (0.90) long and 0.01-0.12 (0.11) wide. Female worms measured 3.12-5.011 (4.30) long and 0.13-0.29 (0.17) wide. Esophagus followed by intestine which opens at the posterior end via anal opening in females and via cloacal opening in males. Males have a single spicule with a gubernaculum and an accessory hook. Females’ uteri were pack with eggs. Data from 18S rDNA revealed a sequence which was identical to Syphacia obvelata in NCBI GenBank. Similarly, sequences from ITS regions grouped with sequences from S. obvelata confirming the morphological identity of the worm. However, it showed 3 mutations at the ITS2 region from related sequences from S. obvelata at NCBI GenBank.
Keywords:
Syphacia obvelata; Mus musculus; 18S rDNA; ITS region; Saudi Arabia
... Fenbendazol is administered orally (20 mg/kg, every 24 h for 5 days), but it can be also applied in drinking water (70 mg/L) or feed (150mg/kg) for 3 cycles based upon a week on-off schedule. Piperazine is recommended to be applied in combination with ivermectin as 2.1 mg/ml piperazine sulphate solution in water for two weeks followed by 0.007 mg/ml ivermectin in third and fourth week (ZENNER, 1998;HRAPKIEWICZ et al., 2013;CHAWLA et al., 2015). ...
The role of rodents as reservoirs of helminths of public health importance is not well known. The zoonotic potential of Syphacia spp. has been confirmed; therefore, the study aimed to estimate the occurrence of oxyurid nematodes in small rodents from pet shops and breeding clubs in Slovakia. Fecal samples of 586 pet rodents kept in 133 cages were collected between 2016 and 2018 and examined by Faust´s flotation method. Four species of oxyurid nematodes, Syphacia muris, S. obvelata, Aspiculuris tetraptera and Paraspidodera uncinata were detected. A. tetraptera was found in the faecal samples of all rodent species included in this survey. The number of positive boxes varied from 5.4% in hamsters to 70.0% with mice. The prevalence of Syphacia muris was highest in Mongolian gerbils where up to 75.0% boxes were positive; S. obvelata was found in 26.7% of boxes with mice, 25.0% of boxes with Mongolian gerbils and 3.2% of boxes with rats. The high prevalence of Syphacia spp. in all animal species points out the infection risk for humans. Animals offered for sale are often in close contact with human beings; therefore they should be regularly tested for parasites and then effectively dewormed.
