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![Pathology images after surgical excision of lesion: [A,B] Hematoxylin and eosin stained sections show a large exophytic lesion consisting of branched fibrovascular cores lined by conjunctival epithelium with numerous goblet cells and intraepithelial neutrophils, [C] Many cells show nuclear p16 immunoexpression (arrows). [D] In situ hybridization for low-risk HPV 6/11 is also present in some cells (arrows).](profile/Anvesh-Annadanam/publication/314240537/figure/fig2/AS:468903525654530@1488806720397/Pathology-images-after-surgical-excision-of-lesion-A-B-Hematoxylin-and-eosin-stained.png)
Pathology images after surgical excision of lesion: [A,B] Hematoxylin and eosin stained sections show a large exophytic lesion consisting of branched fibrovascular cores lined by conjunctival epithelium with numerous goblet cells and intraepithelial neutrophils, [C] Many cells show nuclear p16 immunoexpression (arrows). [D] In situ hybridization for low-risk HPV 6/11 is also present in some cells (arrows).
Source publication
![Figure 1. Gross images of lesion before excision: [A] Fullness of...](profile/Anvesh-Annadanam/publication/314240537/figure/fig1/AS:468903525654528@1488806720250/Gross-images-of-lesion-before-excision-A-Fullness-of-eyelid-B-Exophytic_Q320.jpg)
![Figure 2. Pathology images after surgical excision of lesion: [A,B]...](profile/Anvesh-Annadanam/publication/314240537/figure/fig2/AS:468903525654530@1488806720397/Pathology-images-after-surgical-excision-of-lesion-A-B-Hematoxylin-and-eosin-stained_Q320.jpg)
Background: Most conjunctival papillomas are benign in nature. However, any long standing conjunctival mass should not evade the surveillance of carcinogenicity. Presented here is a case of a long standing
Contexts in source publication
Context 1
... specimen was sent to pathology for further characterization. The histopathology reportconfirmed the mass was a papilloma lined by conjunctival epithelium with numerous goblet cells (Figures 2A and 2B). The lesion was inflamed with extensive neutrophils within the epithelium causing some reactive atypia, but no dysplasia was identi- fied. ...
Context 2
... lesion was inflamed with extensive neutrophils within the epithelium causing some reactive atypia, but no dysplasia was identi- fied. Some koilocytic changes were noted, and nuclear p16 immunostains were positive in many cells (Figure 2C). In situ hybridization revealed a few cells to be positive for low risk HPV 6/11 ( Figure 2D). ...
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Background: Human papillomavirus (HPV) is the major cause of cervical cancer (CC) etiology; its contribution to head and neck cancer (HNC) incidence is steadily increasing. As individual patients’ response to the treatment of HPV-associated cancer is variable, there is a pressing need for the identification of biomarkers for risk stratification tha...
Citations
... Interestingly, prior studies using DNA probes had lower rates of HPV detected, perhaps reflecting the age of the studies [73]. While some single cases reports examined papillomas using RNA in-situ hybridization, this study represents the first series of cases, and the larger percentage of papillomas found to be positive using RNA in-situ hybridization could potentially reflect the increased sensitivity of this approach [74,75]. However, given the relatively small number of cases in each group, the differences between DNA and RNA in-situ approaches could also be due to normal variation. ...
In-situ hybridization provides a convenient and reliable method to detect human papillomavirus (HPV) infection in formalin-fixed paraffin-embedded tissue. Cases of conjunctival papillomas, conjunctival intraepithelial neoplasia (CIN), conjunctival carcinoma in situ (cCIS), and invasive squamous cell carcinoma (SCC), in which low-risk (LR) and/or high-risk (HR) HPV types were evaluated by RNA or DNA in-situ hybridization, were retrospectively identified. LR HPV types were frequently detected in conjunctival papillomas (25/30, 83%), including 17/18 (94%) with RNA probes, compared to 8/12 (75%) with DNA probes. None of the CIN/cCIS or SCC cases were positive for LR HPV by either method. HR HPV was detected by RNA in-situ hybridization in 1/16 (6%) of CIN/cCIS cases and 2/4 (50%) of SCC cases, while DNA in-situ hybridization failed to detect HPV infection in any of the CIN/cCIS lesions. Reactive atypia and dysplasia observed in papillomas was generally associated with the detection of LR HPV types. Collectively, our findings indicate RNA in-situ hybridization may provide a high-sensitivity approach for identifying HPV infection in squamous lesions of the conjunctiva and facilitate the distinction between reactive atypia and true dysplasia. There was no clear association between HPV infection and atopy in papillomas or dysplastic lesions.
... HPV has been identified in several lesions of the ocular surface. A strong association between HPV types 6 and 11 and conjunctival papilloma has been established (49,52,57,59,60). The varied percentages of papillomas associated with HPV presence could be attributed to differences in genetic predisposition, lifestyle and environmental exposure (73). ...
Human papillomavirus (HPV) infection has been implicated as a primary cause of lesions in the anogenital region, skin, oropharynx and respiratory tract. Additionally, the role of HPV in the pathogenesis of ocular surface disease has also been extensively studied. Conjunctival papilloma development has been strongly associated with the HPV infection of certain subtypes. On the other hand, the role of HPV in conjunctival pterygium, conjunctival intraepithelial neoplasia (CIN) and ocular surface squamous neoplasia (OSSN) remains controversial. Genetic predisposition and environmental factor is important in HPV hosts as regards the pathogenesis of ocular surface disease. Several studies have indicate a synergic role of HPV with ultraviolet radiation in pterygium establishment. A higher recurrence risk rate and more aggressive disease of ophthalmic pterygium is observed in cases of HPV infection. The purpose of this review was to provide a systematic review of the literature and to assist in a better understanding of the role of HPV in ocular surface disease.
