Pathology from the kidney mass. Tissue from partial nephrectomy revealed renal cell carcinoma, clear cell type, Fuhrman grade 2, magnified at ×200 (above) and ×400 (below). 

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... to cause hypercalcemia such as interleukin-6 (IL-6), prostaglandins, and tumor ne- crosis factor alpha (TNF- α ). This report describes an unusual case of paraneoplastic hypercalcemic coma occurring with renal cell carcinoma (RCC) and a discussion of the differential diagnosis and laboratory evaluation of this condition. A 62-year-old white woman was admitted to an outside medical facility with profound onset of hypercalcemia causing marked mental status changes. The patient had been in good health until the day of her admission when she was found unconscious at her home. She was transported to the emergency department where she was found to have a serum calcium level of 18.6 mg/dL. Her medical history includes chronic bronchiectasis, atrial fibrillation, duodenal ulcer, depression, urolithiasis, polymyalgia rheumatica, and maxillary sinusitis with history of sinus abscess. Her home medications included weekly rotating antibiotics for chronic sinusitis: ciprofloxacin 500 mg bid, followed by cefuroxime axetil 500 mg bid, followed by trimeth- oprim/sulfamethoxazole 160/800 bid, and then followed by doxycycline 100 mg bid; acetaminophen prn; amitriptyline 50 mg qhs; bumetanide 1 mg daily prn; potassium chloride 60 meq bid; spironolactone 25 mg bid; Premarin® 1.25 mg daily; digoxin 0.25 mg bid; trazodone 150 mg prn insomnia; pantoprazole 40 mg daily; docusate 100 mg TID prn constipation; gabapentin 300 mg qhs; metoprolol extended release 100 mg qhs; and prednisolone 15 mg daily. She was treated with approximately 8 L of normal saline; once daily intravenous (IV) furosemide; six doses of subcuta- neous calcitonin at 100 U every 6 hours; 20 mg of solumedrol twice daily; and 90 mg of IV pamidronate. The following morning her serum calcium was 12.9 mg/dL. By the fifth hospital day, her serum calcium was normal at 8.7 mg/dL, and her mental status normalized. Her evaluation revealed a suppressed parathyroid hormone (PTH) level of 2.7 pg/mL, undetectable PTHrP, and low 1,25-dihydroxyvitamin D (1,25 (OH) 2 D). Her hypercalcemia was thought to be because of an underlying malignancy, and she was discharged with plans for outpatient imaging. One month later, she presented again in an unconscious state with a serum calcium level of 11.0 mg/dL. She was treated again with IV fluids and pamidronate. She underwent a comprehensive search for malignancy, which was negative. CT scans of the head, neck, chest, abdomen, and pelvis did not identify suspicious masses. A total body bone scan and skeletal survey x-ray did not identify osteolytic lesions. An upper endoscopy and colonoscopy did not visualize malignant lesions. Mammogram and thyroid uptake scans were normal. The patient was discharged with plans for evaluation at an outside facility. Within 2 months of her initial presentation, the patient was referred to our academic medical center for further evaluation of her hypercalcemia. She denied any history of fevers, night sweats, hematuria, flank pain, or parathyroid disease but did report mild constipation, a history of nephrolithiasis, and 20- lb unintentional weight loss within the previous 3 months. Physical examination revealed a blood pressure of 124/ 64 mmHg, pulse rate of 84/minute, respiratory rate 18/ minute, and temperature at 97.5°F. Head, ear, nose, and throat examination was normal without thyroid enlargement, carotid bruits, or cervical lymphadenopathy. Pulmonary and cardiovascular exams were normal. No costovertebral angle tenderness or flank masses were found on abdominal exam. Her neurological exam was normal except difficulty with concentration. Her skin had no bruises or rash. Repeat laboratory tests included normal blood counts, electrolytes, transaminases, and alkaline phosphatase. Calcium was elevated to 12.1 mg/dL. Albumin was 3.8 g/dL. Intact PTH was 8 pg/mL (normal 10 – 65 pg/mL), PTHrP was undetectable, midsegment PTHrP was undetectable, 1,25(OH) 2 D was suppressed at 6.7 pg/mL (normal 25 – 66 pg/mL), and 25- hydroxy vitamin D was 52 ng/mL. Twenty-four-hour urine calcium was elevated at 400 mg (normal 100 – 300 mg), and phosphorus was 310 mg (normal 400 – 1300 mg). Calcitonin, thyrotropin, and serum protein electrophoresis were normal. The initial CT images performed at the outside facility were not available for review. We suspected a paraneoplastic syndrome due to an occult malignancy because of her weight loss, hypercalcemia, and suppressed PTH value. Our patient underwent a PET scan to investigate for a potential malignancy, revealing reactive inflammation in subcentimeter mediastinal lymph nodes and a 2.7×2.3-cm exophytic mass at the superior pole of the left kidney, which was not previously reported in prior radiologic imaging. A repeat abdominal CT scan was performed and confirmed the left-sided kidney mass (Fig. 1). She underwent a partial left nephrectomy 10 weeks after her initial presentation. Surgical pathology demonstrated a 3.9-cm RCC, clear cell type with Fuhrman ’ s nuclear grade 2 (Fig. 2), with staging of T1aN0M0. After surgery, she became hypocalcemic and required IV infusion of calcium. She was discharged home 3 days after surgery. Twenty-six days after surgery, she was seen in our outpatient endocrinology clinic with a serum calcium level of 10.7 mg/dL. Two months after her surgery, our patient ’ s serum calcium level was measured at 10.4 mg/dL with an intact PTH level of 45 pg/mL (Fig. 3). Follow-up CT scan of the abdomen revealed no recurrence of the RCC. We analyzed presurgery serum samples for TNF α and IL-6 levels by ELISA (Alpco Diagnostics, Salem, NH, USA); however, both of these cytokines were undetectable. Immunohistochem- istry for IL-6 (Santa Cruz Biotechnology, Inc., Santa Cruz, Calif, USA) and PTH (BioGenex, San Ramon, Calif, USA) was performed on two separate areas of the tumor with adjacent normal kidney. Normal renal parenchyma and parathyroid adenoma served as positive controls for IL-6 and PTH, respectively. Negative controls were performed by replacing the primary antibody with buffer. The RCC was negative for both IL-6 and PTH immunohistochemistry. TNF α and PTHrP stains were not available. Renal cell carcinoma accounts for 2% of cancers worldwide. The incidence of RCC has risen over the past several decades, due largely to incidental detection by imaging modalities such as CT scan, ultrasound, and MRI. 2 Nearly 50% of cases are identified incidentally by imaging, 3 and accuracy of diagnosis with CT scan is greater than 95%. 4 Given this high level of accuracy and the size of her tumor, we must attribute our patient ’ s initial negative CT scan to human error. When we finally obtained the original images, the tumor was, in fact, present. Detection of unknown primary tumors ranges from 33% to 57% with PET-CT, 5 – 7 but there is a paucity of data on the use of PET-CT in paraneoplastic syndromes. The most common clinical presentations of RCC are hematuria (50% to 60% of patients), abdominal pain (40%), and a palpable abdominal mass (30%); this classic triad of symptoms is present in less than 10% of cases. 8 Paraneoplastic manifestations of RCC, including hypercalcemia, polycythemia, hepat- ic dysfunction, amyloidosis, fever, and weight loss, are present in up to 20% of patients. 3,8,9 Unusual presentations of RCC include pancreatitis, 10 life-threatening polyneuropathy, 11 gas- trointestinal bleeding, 12 and polymyalgia rheumatica, 13 but hypercalcemia-induced coma has not been reported. In retrospective studies to determine the etiology of hypercalcemia in all patients, 28 – 36% of cases are attributed to malignancy. 14,15 Of patients with known malignancy who develop hypercalcemia, the most common malignancies are lung and kidney. 15,16 Hypercalcemia is the most common paraneoplastic complication of RCC, and approximately 17% of all patients with RCC develop hypercalcemia during the course of their disease. 17 – 19 Albright 20 first reported hypercalcemia occurring in a patient with RCC in 1941. He postulated that the hypercalcemia was because of a humoral factor secreted by the tumor. Later reports confirmed that a protein was secreted from RCC, which could increase urinary cyclic adenosine monophosphate levels similar to PTH. 21,22 In the late 1980s, the substance was identified and named PTHrP. 23 Parathyroid hormone-related peptide is present in several isoforms. The N-terminal sequence is identical in 8 of the first 13 amino acids compared to PTH and binds to the PTH/PTHrP receptor. 24 Parathyroid hormone-related peptide undergoes posttranscriptional modification, dividing itself into the N- terminal fragment, midsection, and C-terminal fragment. 25 The C-terminal fragment is cleared by the kidney and is present in higher serum concentrations, particularly in kidney failure. There is some evidence that the C-terminal fragment inhibits RCC growth, whereas the N-peptide stimulates growth. 24,26 In addition, the C-peptide plays an inhibitory role in calcium metabolism. 26 It is reported that PTHrP levels are elevated in up to 47% of patients with malignancy and hypercalcemia. 27 Parathyroid hormone-related peptide is elevated in up to 15% of all patients with RCC, 19 but it is also expressed in normal tissue. 28 This peptide binds to the common PTH/PTHrP receptor to cause increased resorption of bone and increased renal calcium absorption. 29 – 31 Other humoral factors that have been associated with hypercalcemia in RCC include IL-6, IL-1, TNF α , and trans- forming growth factor alpha and beta. 32,33 Many studies have attempted to determine the exact role of IL-6 in hypercalcemia of RCC. Interleukin-6 has been shown to activate osteoclastic bone resorption, and it acts synergistically when coexpressed with PTHrP. Some studies suggest that IL-6 stimulates tumor growth. 34,35 It is not clear whether this cytokine causes hypercalcemia either directly or indirectly by increasing the effect of PTHrP on bone resorption. 36 – 38 At this time, IL-6- mediated ...