Pathological morphology of different tissues observed under microscope. In mice treated with TiO 2 NPs, neuronal cell degeneration was observed in the brain tissue; vacuoles were observed in the neurons of hippocampus and their number was increased in the high dose groups, which indicated fatty degeneration occurred in the hippocampus of brain tissue. In the lung tissues, perivascular infiltration of inflammatory cells, foamy cells as well as pulmonary fibrosis were observed; the granulomatous lesions were found at the doses of 645 and 1387 mg/ kg. At the doses of 140 and 300 mg/kg, TiO 2 NPs showed vacuolar degeneration in the liver. At 645 mg/kg, inflammatory cells were found in the bile ducts of the liver, and hydropic degeneration around the central vein and spotty necrosis of hepatocytes were also observed. At 645 and 1387 mg/ kg, multifocal lesions were observed in the liver. In the kidneys, swelling in the renal glomerulus was observed in TiO 2 NPs treated mice. In the spleen, minor lesions were observed due to increased proliferation of local macrophages. No obvious abnormality in histology was observed in the heart in TiO 2 NPs treated mice as seen under the microscope (400 6 ). doi:10.1371/journal.pone.0070618.g003 

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... Method. After mixing with a vortex, a single injection of TiO 2 NPs saline suspension was administered through the tail vein (28 G needle). Control group mice were given saline only. Behavior and mortality were monitored and recorded carefully after treatment. 14 days after treatment, blood samples were collected from the femoral artery by a quick incision of the artery while the animal was anesthetized with 2% isoflurane. The animals were then euthanized by cervical dislocation. Serum was obtained by centrifugation at 3500 rpm for 10 minutes and stored at 2 80 u until used. Bone marrow smears were prepared by smearing a mixture of mouse bone marrow from the femur and calf serum on a coated glass slide. Slides were air dried and then fixed in methanol for 15 minutes for the micronucleus test. The organs (heart, lung, liver, spleen, kidneys and brain) were excised and weighed accurately. A small piece of tissue from each organ was dissected, fixed in a 6% formalin solution and stored at 4 u C until used. After weighing the organs, the organ weight/BW coefficients of heart, lung, liver, spleen, kidneys and brain were calculated as organ weight (wet weight, mg)/BW (g) 6 100%. Biochemical parameters detected in blood serum of mice included total bilirubin levels (TBIL), indirect bilirubin (IBIL), direct bilirubin (DBIL), alkaline phosphatase (ALP), alanine aminotransferase (ALT) and aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (CREA), uric acid (URCA) and the enzyme creatine kinase (CK). Hematological parameters examined in this study included WBC count, red blood cell (RBC) count, hemoglobin (HGB), red blood cell specific volume (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red cell distribution width (RDW-CV), platelet (PLT), platelet distribution width (PDW-CV), mean platelet volume (MPV) and plateletcrit (PCT). The fixed tissues were stored at 4 C overnight and then embedded in paraffin blocks. 4 m m thick tissue sections were prepared and stained with hematoxylin and eosin (H&E). Histopathological morphology was checked under the microscope by an independent pathologist. The methanol fixed bone marrow smears were stained with Giemsa stain according to the conventional method. One thousand polychromatic erythrocytes per animal were analyzed for the presence of micronucleus in cells. Cyclophosphamide (20 mg/kg, two intraperitoneal injections, at 24 and 48 h before mice were sacrificed, respectively) was used as a positive control. Results were expressed as mean 6 standard deviation (SD). Multigroup comparisons of the means were carried out by one- way analysis of variance (ANOVA) test. Dunnett’s test was used to compare the difference between the experimental groups and the control group. The statistical significance for all tests was set at P , 0.05. The average size distribution of TiO 2 NPs was 42.30 6 4.60 nm as detected by optimas 6.5 image analysis software. Image of TiO 2 NPs was captured by SEM (Figure 1). The size distribution of TiO 2 NP aggregates in saline was observed under the light microscope (Figure 2). 7 days after treatment, difference in eating and drinking patterns and physical activity were observed in the 1387 mg/kg dose group. They showed decreased food and water intake and decreased physical activity than control group. At day 9, 2 males and 4 females in the 1387 mg/kg dose group died. 2 males survived at this dose level, leaving less than three mice at the end of the experiment. Therefore, in this dose group only the tissues were histopathologically analyzed. Data were expressed as means 6 SD (n = 4) (Table 2). Comparisons were carried out by one-way variance test (SNK’s multiple comparison tests). Compared to control, coefficients of the spleens in TiO 2 NPs treated mice increased significantly; however, coefficients of the liver and kidney decreased significantly. No significant effects were observed in the coefficients of the heart, lung or brain in TiO NPs treated mice. Biochemical parameters in the serum as detected by the autoanalyzer are listed in Table 3. No significant differences were found in the serum levels of TBIL, ALT, AST, ALP, BUN, CREA, or CK in TiO 2 NPs treated mice as compared to the control group. The levels of DBIL and IBIL in TiO 2 NPs treated mice decreased in a dose dependent manner. The level of URCA in TiO 2 NPs treated mice at 140 and 300 mg/kg were significantly increased compared to the control group. Hematological parameters in the blood as detected by the autoanalyzer are listed in Table 4. Intravenous injection of high doses of TiO 2 NPs did not induce significant acute hematological toxicity except for an increase in the WBC count in 645 mg/kg dose group. Histopathological examination of the tissues indicated that intravenous administration of high doses of TiO 2 NPs could induce multi-organ pathological lesions in a dose dependent manner (Figure 3). The results show that TiO 2 NPs treatment could induce different degrees of damage in the brain, lung, spleen, liver and kidneys. However, no obvious pathological effects were observed in the heart of TiO NPs treated mice. Micronucleus test result 14 days after a single intravenous injection of different doses of TiO 2 NPs shows, no significant increase in micronucleus cell number in the polychromatic erythrocytes among TiO 2 NPs treated mice as compared to the control (Figure 4). Studies have demonstrated that accumulation of TiO 2 NP can be observed in the liver, lung, kidneys, and spleen after intraperitoneal, intravenous or dermal administration.[13–15] After inhalation exposure in rats, TiO 2 NPs have been found to accumulate in the lung, leading to phagocytosis [16,17]. Wang et al. [18] found that a single oral gavage exposure of a very high dose (5 g/kg) of TiO 2 NPs (80 nm) in mice could elevate the ALT/AST enzyme ratio and LDH level in serum, which implies TiO 2 NPs may induce hepatic injury. Fabian et al. [12] reported the accumulation of TiO 2 NPs in the liver, spleen, lung, and kidneys after intravenous administration of 5 mg/kg TiO 2 NPs in rat. However, there were no remarkable toxic effects observed in these organs. In this study, behavioral and physical symptoms of acute toxicity such as decreased activity or decreased uptake of food and water were observed in the first week in the mice treated with 1387 mg/ kg of TiO 2 NPs. The behavior of the mice in the other dose groups was normal throughout the study. Compared to the control group, organ coefficients of the spleens in TiO 2 NPs treated mice increased significantly; while, the organ coefficients of the liver and kidneys decreased significantly. Both increase and decrease of the organ coefficients may be caused by TiO 2 NPs excretion or accumulation in the organs, which could cause certain histopathological changes. These changes were confirmed in the histopathological tissue sections of the liver and kidneys. The liver tissue showed hepatocyte vacuolar degeneration, spotty necrosis of liver hepatocytes, along with inflammatory cell invasions in the bile ducts of the liver, and hydropic degeneration around the central vein. At higher doses, multifocal lesions were also observed in the liver. Wang et al. [18] reported that TiO 2 NPs treatment through oral administration could increase hepatocyte necrosis. Combined with our results, it can be concluded that TiO 2 NPs can induce hepatocyte injury in vivo . In the kidneys, swelling in the renal glomerulus in TiO 2 NPs treated mice was observed. Serum biochemical analysis showed a significant increase in blood URCA level in TiO 2 NPs treated mice. URCA is the end product of decomposition of a purine nucleic acid, which is mainly excreted from the kidneys. Blood URCA levels are elevated in renal dysfunction [19]. In the spleen, TiO 2 NPs treatment caused increased proliferation of local macrophages, which is consistent with the results of the increased organ coefficients. Although no significant changes were found in the organ coefficients for brain and lung tissues in TiO 2 NPs treated mice, histopathological examination showed certain lesions. The brain tissue showed neuronal cell degeneration and vacuoles were observed in the hippocampus, which is indicative of fatty degeneration in the hippocampus. In the lung tissues, inflammatory cells, foamy cells and granulomatous lesions were observed. Park at al. [20] reported that granulomatous lesions were found in the bronchiole and alveoli of the lung after 14 days TiO 2 NPs treatment (5 mg/kg, 20 mg/kg, and 50 mg/kg) in mice. Rours- gaard et al. [21] also found inflammation in the lungs after TiO 2 NPs treatment in mice by intratracheal instillation. Bilirubin consists of TBIL, IBIL, and DBIL. Studies have revealed that the levels of serum bilirubin are inversely related to the risk of certain heart diseases [22]. The results showed that both IBIL and DBIL significantly decreased in TiO 2 NPs treated mice compared to the control mice, which suggests that TiO 2 NPs treatment might also have myocardial effects. However, histopathological examination of the heart showed no pathological effects and no significant changes in the organ coefficients in the TiO 2 NPs treated mice in this study. These differences may be attributed to late onset of histological evidence of myocardial pathology. Hematological analysis is normally used to detect the hematological toxicity of different chemicals. In this study, the results indicated that, 14 days after intravenous administration of the high dose of TiO 2 NPs, no significant hematological toxicity could be observed. The micronucleus test is frequently used as a tool for genotoxicity assessment of various chemicals. It is easier to conduct than the chromosomal aberration test in terms of procedures and evaluation. In this study, 14 days after a single intravenous injection of different doses of ...