Fig 3 - uploaded by Ronnie Concepcion II
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Paper chromatography setup for segmenting phytopigments into clear molecular chromatic components (a). Distribution gradient of chlorophylls and carotenoids in a chromatography paper (b).
Source publication
Phytopigments are essential indicators of plant growth. However, current methodologies use expensive laboratory devices. In this study, a low-cost approach of lettuce leaf phytopigments profiling is employed using a consumer-grade camera and integrated computational intelligence via paper chromatography. Hybrid neighborhood component analysis and R...
Contexts in source publication
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... To make these pigments spread easily with the chromatography paper, 0.1 mL of acetone ((CH3)2CO) is dissolve with it. Customized chromatography paper was made using 90 sets of cellulose Leaf phytopigment reflectance and spectro-texturalmorphological characterization Phytopigment profiling filter papers (Whatman) with dimensions specified in Fig. 3b. In paper chromatography, stationary phase starts with leaf stain placed on the central 1 cm mark from the bottom edge of the paper using a capillary tube. Leaf stained-paper is placed inside the 100 mL clear glass graduated cylinder (Bomex, China), with 0.5 cm fold at the top edge for hanging, using a steady nylon thread (Fig. 3a). ...
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... specified in Fig. 3b. In paper chromatography, stationary phase starts with leaf stain placed on the central 1 cm mark from the bottom edge of the paper using a capillary tube. Leaf stained-paper is placed inside the 100 mL clear glass graduated cylinder (Bomex, China), with 0.5 cm fold at the top edge for hanging, using a steady nylon thread (Fig. 3a). Petri dish was used to close the fluid system inside the cylinder resulting to faster pigment gradient. The mobile phase starts moving when the 10 mL acetone polar solvent interacts with leaf stain and separates leaf pigments in upward direction from Chl b to Car (Fig. 3b). It stops when no further pigment gradients is appeared and ...
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... with 0.5 cm fold at the top edge for hanging, using a steady nylon thread (Fig. 3a). Petri dish was used to close the fluid system inside the cylinder resulting to faster pigment gradient. The mobile phase starts moving when the 10 mL acetone polar solvent interacts with leaf stain and separates leaf pigments in upward direction from Chl b to Car (Fig. 3b). It stops when no further pigment gradients is appeared and usually happens after 15 minutes. ...
