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PGT2/Quercetin Reaction Product on Cellulose TLC Plate in 15% Acetic Acid. Ethyl acetate extract from PGT2/quercetin reaction was co-chromatographed on cellulose TLC plate, using 15% acetic acid as solvent against quercetin 3-O-glucoside as standard. Note: Rf values for PGT/quercetin reaction product were compared to the values obtained from flavonol specifc-3O-GT (Owens and McIntosh, 2009).
Source publication
Expression and Biochemical Function of Putative Flavonoid GT Clones from Grapefruit and Identification of New Clones using the harvEST Database by Venkata K.P.S. Mallampalli Flavonoids are plant secondary metabolites well known for many key roles in the life cycle of plants. They also can affect human health. Citrus paradisi is known to produce sev...
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Context 1
... experiments to identify the PGT2 enzyme reaction product with quercetin as the substrate using co-chromatography on cellulose TLC plates with 15% acetic acid indicated that the product was not quercetin-3-O-glucoside. The highest peak of activity of PGT2 did not match the location of quercetin-3-O-glucoside on the cellulose TLC plate (Figure 12). The Rf value for PGT2 reaction product, quercetin, and quercetin-3-O-glucoside were 28, 0, and 24 respectively ( Figure 12). ...
Context 2
... highest peak of activity of PGT2 did not match the location of quercetin-3-O-glucoside on the cellulose TLC plate (Figure 12). The Rf value for PGT2 reaction product, quercetin, and quercetin-3-O-glucoside were 28, 0, and 24 respectively ( Figure 12). Identification of reaction product is in progress. ...
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Citations
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