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PCR primers and characteristics of 10 scnDNA markers developed for Euchresta horsfieldii.
Source publication
Premise of the Study
Euchresta horsfieldii (Fabaceae) is a rare and endangered medicinal plant in Indonesia with restricted distribution. Single‐copy nuclear DNA (scnDNA) markers were developed for this species to facilitate further investigation of genetic diversity and population structure.
Methods and Results
We performed RNA‐Seq and de novo as...
Contexts in source publication
Context 1
... specimens of E. horsfieldii were deposited in the Herbarium Hortus Botanicus Baliense (THBB), Bali Botanic Garden, Indonesian Institute of Sciences (LIPI), Bali, Indonesia (Appendix 1). PCR primer pairs and characteristics of the 10 newly developed scnDNA markers, GenBank accessions, and BLASTN hits are presented in Table 1 ...
Context 2
... Sequence information for the developed primers has been deposited to NCBI; GenBank accession numbers are provided in Table 1. Note: A = number of alleles; h = haplotype diversity; k = average number of nucleotide differences; n = number of sequences used (consists of four sequences of E. japonica, 12 sequences of E. tubulosa, and 80 sequences of E. horsfieldii); p n = proportion of polymorphic sites; π = nucleotide diversity; θ w = Watterson estimator per site from S; S = variable sites. ...
Citations
... Euchresta horsfieldii is a rare medicinal plant [5,6], and need to be prioritized for conservation [7]. Medicinal properties of this plant species have been reported [8][9][10][11][12][13]. ...
... Research on Pronojiwo stem cuttings with regard to the number of internodes and growth regulators has been successfully carried out [5]. In addition, research involving DNA manipulation has been carried out to facilitate further research on genetic diversity and population structure of the Pronojiwo plant [6] . ...
The Pronojiwo plant (Euchresta horsfieldii (Lesch.) Benn.) is well known as one of the Indonesian medicinal plants that has various health benefits. Pronojiwo grows in the highlands and is commonly found in Bali and NTB. Several researches using samples of Pronojiwo originating from Bali, proved them to have roles as antibacterial, antioxidant, and aphrodisiac. Unfortunately, this plant has been declared as a rare medicinal plant due to the limited distribution of habitat, low natural reproductive ability, and over-exploitation by the people. Pronojiwo plants are also found in Tawangmangu. The potential of Pronojiwo originating from Tawangmangu as medicinal plants has not been studied. This study will examine the presence of the bioactive compounds of the Pronojiwo plant, found in the Tawangmangu sub-district. Fresh samples of leaves and flowers were taken from two location at Tawangmangu. Samples were then extracted by the maceration method and qualitatively tested for alkaloids, flavonoids, tannins, phenolics, saponins, steroids, and triterpenoids. The result shows that both Pronojiwo leaves and flowers contain alkaloids, tannis, flavonoid, phenolic acids, and saponin. In addition, some environmental parameters of the habitat were also observed.
... This plant can be found in Bhutan, China, India, Indonesia, Laos, Nepal, the Philippines, Thailand, and Vietnam. 2 Research on Kopsia arborea Blume. and Euchresta horsfieldii (Lesch.) ...
Background: Purnajiwa is one of the medicinal plants in Bali. Species information regarding this plant is still unconfirmed. Currently, this plant is considered rare and endangered. Molecular identification supports authentication at the species level, which will also support conservation efforts. Method: Molecular identification using DNA Barcoding was carried out on purnajiwa from three sampling locations in Bali (Jimbaran, Mambal, and Bedugul) using rbcL primers (rbcLaF and rbcLaR). Data analysis was performed using BLAST with species on GenBank. Pairwise and similarity values were used to measure the three samples' proximity to the Gen Bank species. Result: The phylogenetic tree was constructed with the Maximum Likelihood and Tamura-3-parameter model. The analysis results show a close relationship between the purnajiwa from Jimbaran and Mambal with the species Kopsia arborea (KP095079). Meanwhile, the Purnajiwa from Bedugul showed a difference from the other two purnajiwa and was closely related to Euchresta horsfieldii (U74225). Conclusion: It can be concluded that the Purnajiwa from Jimbaran and Mambal is a species of Kopsia arborea while the Purnajiwa from Bedugul is a species of Euchresta horsfieldii.
... Biji dari jenis tumbuhan ini dipercaya sebagai bahan pembangkit stamina pria (afrodisiak) (Oktavia et al., 2017;Darma et.al., 2019), sebagai anti bisa ular dan mengobati penyakit TBC (Sutomo dan Mukarromah, 2010). Beberapa penelitian juga menunjukkan E. horsfieldii berpotensi sebagai antibakteri (Prihantini et al., 2018), antitumor, menurunkan kolesterol (Priyadi et al., 2018), antioksidan, menghambat alpha-glukosidase (potensi untuk antidiabetes) (Prihantini et al., 2019), dan mencegah stres oksidatif (Gunawan et al., 2016). ...
Pranajiwa (Euchresta horsfieldii (Lesch.) Benn.) has been widely used as traditional medicine by the Balinese ethnic community. Up to now, the raw materials for traditional medicines from E. horsfieldii are harvested directly from their natural habitat. It is feared that it will interfere with its sustainability. The Balinese ethnic community has a culture known as the Tri Hita Karana (THK) concept, which means there is a balance in the relationship between humans and God, nature, and with fellow humans. This study aims to identify the use of E. horfieldii and its conservation efforts based on THK. This research was carried out in communities living around Bedugul, Bali. The method of data collection was done by participatory observation and semi-structured interviews. The research informants were 33 people with a purposive sampling method. The criteria are based on the level of knowledge and use of E. horsfieldii. Data were analyzed and interpreted descriptively. The results of this study indicate that E. horsfieldii has been widely used to treat various diseases and is practiced by many traditional healers (balian). However, people also use it for treatment without a standardized dose. This causes the measure of its use is very diverse. Harvesting of E. horsfieldii is carried out by the community while still observing the principle of sustainability according to the THK philosophy. Harvesting of E. horsfieldii is mostly done for individual needs. Although there has been a practice of buying and selling E. horsfieldii, harvesting has not resulted in over-exploitation. Efforts to conserve E. horsfieldii have been carried out by the community, especially the balians by trying to cultivate it, although they still fail. In addition, the conservation of E. horsfieldii is carried out by the Bali BKSDA with efforts to maintain the habitat of E. horsfieldii in forest areas.
... Biji dari jenis tumbuhan ini dipercaya sebagai bahan pembangkit stamina pria (afrodisiak) (Oktavia et al., 2017;Darma et.al., 2019), sebagai anti bisa ular dan mengobati penyakit TBC (Sutomo dan Mukarromah, 2010). Beberapa penelitian juga menunjukkan E. horsfieldii berpotensi sebagai antibakteri (Prihantini et al., 2018), antitumor, menurunkan kolesterol (Priyadi et al., 2018), antioksidan, menghambat alpha-glukosidase (potensi untuk antidiabetes) (Prihantini et al., 2019), dan mencegah stres oksidatif (Gunawan et al., 2016). ...
Pranajiwa (Euchresta horsfieldii (Lesch.) Benn.) has been widely used as traditional medicine by the Balinese ethnic community. Up to now, the raw materials for traditional medicines from E. horsfieldii are harvested directly from their natural habitat. It is feared that it will interfere with its sustainability. The Balinese ethnic community has a culture known as the Tri Hita Karana (THK) concept, which means there is a balance in the relationship between humans and God, nature, and with fellow humans. This study aims to identify the use of E. horfieldii and its conservation efforts based on THK. This research was carried out in communities living around Bedugul, Bali. The method of data collection was done by participatory observation and semi-structured interviews. The research informants were 33 people with a purposive sampling method. The criteria are based on the level of knowledge and use of E. horsfieldii. Data were analyzed and interpreted descriptively. The results of this study indicate that E. horsfieldii has been widely used to treat various diseases and is practiced by many traditional healers (balian). However, people also use it for treatment without a standardized dose. This causes the measure of its use is very diverse. Harvesting of E. horsfieldii is carried out by the community while still observing the principle of sustainability according to the THK philosophy. Harvesting of E. horsfieldii is mostly done for individual needs. Although there has been a practice of buying and selling E. horsfieldii, harvesting has not resulted in over-exploitation. Efforts to conserve E. horsfieldii have been carried out by the community, especially the balians by trying to cultivate it, although they still fail. In addition, the conservation of E. horsfieldii is carried out by the Bali BKSDA with efforts to maintain the habitat of E. horsfieldii in forest areas.
