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Ossicaulis lachnopus , mature basidiocarps (Stu ž ica, PRM 899407)). The ochre to rusty brown tinge of lamellae is caused by climatic conditions (dry weather, night frost in late season). Photo J. Holec
Source publication
Identity of Ossicaulis lachnopus and O. lignatilis was studied using both classical and molecular methods. While O. lignatilis is a well-known species, O. lachnopus is almost unknown, having been resurrected to modern mycology by M. Contu in 2000. Based on collections from six European countries, both classical and molecular methods independently p...
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Four new species of Entoloma from Kon Chu Rang Nature Reserve and Ta Dung National Park were discovered during an investigation of the diversity of the mycobiota of Central Vietnam and are described here on the base of the molecular and morphological data. Phylogenetic analysis was based on nrITS1-5.8S-ITS2, nrLSU and tef1α regions. Illustrated des...
Citations
... Contu, O. lignatilis (Pers.) Redhead and Ginns, O. yunnanensis L.P. Tang, N.K. Zeng and S.D. Yang, and O. salomii Siquier and Bellanger (Contu, 2007;Holec and Kolarík, 2013;Yang et al., 2018;Crous et al., 2019). The genus is distinguished by brownrot fungus, adnexed, adnate, or at most subdecurrent lamellae, central to the eccentric stipe, regular trama, small and ellipsoid basidiospores, coralloid hyphae in the epicutis, presence or absence of cheilocystidia, and presence of clamp connections (Holec and Kolarík, 2013). ...
... Redhead and Ginns, O. yunnanensis L.P. Tang, N.K. Zeng and S.D. Yang, and O. salomii Siquier and Bellanger (Contu, 2007;Holec and Kolarík, 2013;Yang et al., 2018;Crous et al., 2019). The genus is distinguished by brownrot fungus, adnexed, adnate, or at most subdecurrent lamellae, central to the eccentric stipe, regular trama, small and ellipsoid basidiospores, coralloid hyphae in the epicutis, presence or absence of cheilocystidia, and presence of clamp connections (Holec and Kolarík, 2013). ...
... Basidiospores ( has slightly smaller basidiospores (2.6-3.0 × 1.8-2.0 µm) than O. borealis (3.0-4.0 × 2.0-2.4 µm) (Holec and Kolarík, 2013;Yang et al., 2018). In terms of habitat, O. yunnanensis grows on the living tree trunk of Rhododendron, whereas O. borealis grows on the dying or decaying tree trunks such as O. lachnopus and O. lignatilis (Holec and Kolarík, 2013;Yang et al., 2018). ...
Background:
Gerhardtia and Ossicaulis are two genera within the family Lyophyllaceae, which show an apparently poor species diversity worldwide. During the field investigation on wild macrofungi, six interesting collections within Gerhardtia and Ossicaulis genera are discovered in the northeastern China.
Methods:
To identify whether these collections of Gerhardtia and Ossicaulis are novel species, we performed phylogenetic analyzes using the following DNA regions: the internal transcribed spacer (ITS) region and the large subunit nuclear ribosomal RNA (nrLSU) region. Moreover, a traditional morphological method also be conducted based on both the macro-morphological and micro-morphological features.
Results:
The results indicated that these collections tested formed two independent lineages in each genus with a high support. In addition, they can easily be separated from all other taxa of the two genera in morphology. Based on the combination of morphological and molecular data, Gerhardtia tomentosa and Ossicaulis borealis, are confirmed as two new species to science.
Discussions:
This study provided a theoretical basis is for the two lyophylloid genera and indicated that the biodiversity resources of northeastern China might be underestimated.
... PRM collections analyzed by M. Kolařík (MK). DNA for the ITS (ITS1, 5.8S, ITS2) barcode sequencing was extracted as described in Holec & Kolařík (2013). PCR amplification of the ITS region sequence was done using the primers ITS1F-KYO2 and ITS4 as described in Kolařík & Vohník (2018). ...
A striking species of cyphelloid fungus tentatively identified as a Henningsomyces or Rectipilus was collected in Białowieża virgin forest in Poland since 1962. After search in literature, morphological studies and phylogenetic analyses based on LSU and ITS rDNA sequences, the species turned out to be unknown to science with ≥ 5% difference in LSU rDNA sequence from nearest representatives of both cyphelloid and agaricoid fungi. We describe it here as a new genus Cyphelloporia and new species C. bialoviesensis. The species is still known only from the Białowieża forest, where it produces large basidiomata consisting of a well developed subiculum and densely aggregated, tubular receptacles up to 10 mm long on decaying trunks of Picea abies. A detailed morphological description accompanied by line drawings and photographs is provided, and differences from similar Rectipilus and Henningsomyces species are discussed. Phylogenies based on LSU and ITS sequences show that C. bialoviesensis belongs to the lineage formed by Rectipilus species together with Phyllotopsis nidulans and Pleurocybella porrigens, whereas true Henningsomyces species belong to a highly unrelated lineage labelled Niaceae or Cyphellaceae. Family position of C. bialoviesensis is discussed showing that Phyllotopsidaceae is well justified.
... Vesterholt 2008a). However, Holec & Kolařík (2013) confirmed that these two are distinct species based on morphological and molecular studies. The material from Gozo (SM450) corresponded to O. lachnopus based on the small spores (3-4 µm long for O. lachnopus; 4-6 µm for O. lignatilis); beige-grey colour of the stipe (almost white in O. lignitis) and natural habitat in forest and maquis (artificial habitats such as parks and garden for O. lignitis). ...
... The material from Gozo (SM450) corresponded to O. lachnopus based on the small spores (3-4 µm long for O. lachnopus; 4-6 µm for O. lignatilis); beige-grey colour of the stipe (almost white in O. lignitis) and natural habitat in forest and maquis (artificial habitats such as parks and garden for O. lignitis). Interestingly, more than half of the collections examined by Holec & Kolařík (2013) turned out to be O. lachnopus indicating that it is not a rare species as formerly believed and previous records of O. lignatilis, should be revised. ...
Mifsud, S. & Mifsud, D.: The macrofungi of Gozo (Maltese Islands): an annotated checklist.-Borziana 4: 51-94. 2023.-ISSN: 2724-5020 online. Records of macrofungi from Gozo are few and underrepresented when compared to those found in mainland Malta. An investigation on the macrofungi occurring on Gozo has been carried out to narrow this knowledge gap. During the present study, 147 samples were collected from Gozo between October 2019 and April 2021. From this sampling effort, 70 different species of macro-fungi were identified, of which 61 were new records for Gozo and 45 were new records for the Maltese Islands. Five of these new records (one new to science) were already published during the progress of this work, whereas the remaining 40 new records are reported for the first time in this account. This investigation elevated the number of macrofungi in Gozo from 36 (prior to the onset of this work) to 97 species. For each of the 40 new records, a detailed account of the examined material, ecology, macro-and micro-morphology, and notes related to the taxon-omy and closely related species are provided. An annotated checklist of all the 97 species occurring in Gozo is given, and details on the locations of each collection are also included. The main threats that local macrofungi are currently facing in Malta and Gozo are for the first time exposed, and conservation measures are discussed.
... The genus of Ossicaulis Redhead & Ginns was erected in 1985, with a north temperate distribution, and four species are known worldwide [20]. It is mainly characterized by adnate, sub-decurrent, or lamellae centrally adnexed to the eccentric stipe, tiny ellipsoid spores, and the presence of clamp connections [21]. ...
The lyophylloid agarics are a group of ecologically highly diversified macrofungi, some of which are very popular edible mushrooms. However, we know little about lyophylloid species diversity in China. In this study, we described four new species from China: Lyophyllum atrofuscum, L. subalpinarum, L. subdecastes, and Ossicaulis sichuanensis. We conducted molecular phylogenetic analyses of Lyophyllaceae based on the nuclear ribosomal RNA gene (nLSU) and the internal transcribed spacer regions (ITS). Phylogenetic analyses by the maximum likelihood method and Bayesian inference showed that the four new species are unique monophyletic species. A key to the species of Lyophyllum from China and a key to Ossicaulis worldwide were given.
... Ossicaulis lignatilis -Der Holz-Trichterling ist eine Art der Auwälder und der Buchenwälder. Durch den Rückgang von natürlichen Auwäldern ist eine negative Bestandsentwicklung zu erkennen, die Art kann aber auch in vom Menschen gemachten Habitaten wie Parks oder Alleen vorkommen(Holec & Kolařík 2013;Kriegelsteiner & Gminder 2010). Die Art wächst auf Stümpfen oder Strünken von verschiedenen Laubholzarten(Kriegelsteiner & Gminder 2010). ...
In this research project, we investigated relationships between species and ecosystems associated with wilderness areas in Germany, as defined by the German National Strategy for Biodiversity. Guiding research questions were: 1) Which species and biocoenosis rely on wilderness-related ecosystem characteristics and processes?, 2) How can these species (animals, plants, fungi) be classified to species groups based on wilderness-related criteria?, 3) Which flagship, umbrella, and keystone species can be identified in this context?, 4) Which characteristics of wilderness areas are necessary to provide favourable habitats for wilderness-typic species groups?, and 5) Which natural factors, including disturbance regimes, promote habitat characteristics in wilderness areas, on which these species and biocoenosis are dependent?
We classified over 635 plants, 1,435 animal, and 44 fungi based on 50 wilderness-related criteria into obligatory species (species dependent on wilderness, class A), facultative species (species promoted by wilderness or wilderness-related processes, class B), and other species (species that have their core distribution in habitats of cultural landscapes and are not related to wilderness, class C). As one result, we identified five ecological species groups, for example species of open habitats in coastal and river landscapes that have a regular disturbance regime. Most animals and fungi rely on old-growth forests with high amounts of deadwood, while most plants associated with wilderness were are related to bogs and dynamic riparian ecosystems. These and the other results of the project will support the future selection of suitable areas for wilderness development in otherwise human-dominated landscapes in Europe.
... The new species occupies a basal position in the ITS phylogeny, which may support a dedicated genus. However, in addition to the LSU data, the gross morphology, anatomy, organoleptic features and ecology of the Balearic collections, fit well with the classic delineation of Ossicaulis (Holec & Kolařík 2013). With O. lachnopus, O. salomii shares the shape, but not the size, of the spores; with O. lignatilis, spore calibre but not the shape. ...
Novel species of fungi described in this study include those from various countries as follows: Australia, Chaetomella pseudocircinoseta and Coniella pseudodiospyri on Eucalyptus microcorys leaves, Cladophialophora eucalypti, Teratosphaeria dunnii and Vermiculariopsiella dunnii on Eucalyptus dunnii leaves, Cylindrium grande and Hypsotheca eucalyptorum on Eucalyptus grandis leaves, Elsinoe salignae on Eucalyptus saligna leaves, Marasmius lebeliae on litter of regenerating subtropical rainforest, Phialoseptomonium eucalypti (incl. Phialoseptomonium gen. nov.) on Eucalyptus grandis × camaldulensis leaves, Phlogicylindrium pawpawense on Eucalyptus tereticornis leaves, Phyllosticta longicauda as an endophyte from healthy Eustrephus latifolius leaves, Pseudosydowia eucalyptorum on Eucalyptus sp. leaves, Saitozyma wallum on Banksia aemula leaves, Teratosphaeria henryi on Corymbia henryi leaves. Brazil, Aspergillus bezerrae, Backusella azygospora, Mariannaea terricola and Talaromyces pernambucoensis from soil, Calonectria matogrossensis on Eucalyptus urophylla leaves, Calvatia brasiliensis on soil, Carcinomyces nordestinensis on Bromelia antiacantha leaves, Dendryphiella stromaticola on small branches of an unidentified plant, Nigrospora brasiliensis on Nopalea cochenillifera leaves, Penicillium alagoense as a leaf endophyte on a Miconia sp., Podosordaria nigrobrunnea on dung, Spegazzinia bromeliacearum as a leaf endophyte on Tilandsia catimbauensis, Xylobolus brasiliensis on decaying wood. Bulgaria, Kazachstania molopis from the gut of the beetle Molops piceus. Croatia, Mollisia endocrystallina from a fallen decorticated Picea abies tree trunk. Ecuador, Hygrocybe rodomaculata on soil. Hungary, Alfoldia vorosii (incl. Alfoldia gen. nov.) from Juniperus communis roots, Kiskunsagia ubrizsyi (incl. Kiskunsagia gen. nov.) from Fumana procumbens roots. India, Aureobasidium tremulum as laboratory contaminant, Leucosporidium himalayensis and Naganishia indica from windblown dust on glaciers. Italy, Neodevriesia cycadicola on Cycas sp. leaves, Pseudocercospora pseudomyrticola on Myrtus communis leaves, Ramularia pistaciae on Pistacia lentiscus leaves, Neognomoniopsis quercina (incl. Neognomoniopsis gen. nov.) on Quercus ilex leaves. Japan, Diaporthe fructicola on Passiflora edulis × P. edulis f. flavicarpa fruit, Entoloma nipponicum on leaf litter in a mixed Cryptomeria japonica and Acer spp. forest. Macedonia, Astraeus macedonicus on soil. Malaysia, Fusicladium eucalyptigenum on Eucalyptus sp. twigs, Neoacrodontiella eucalypti (incl. Neoacrodontiella gen. nov.) on Eucalyptus urophylla leaves. Mozambique, Meliola gorongosensis on dead Philenoptera violacea leaflets. Nepal, Coniochaeta dendrobiicola from Dendriobium lognicornu roots. New Zealand, Neodevriesia sexualis and Thozetella neonivea on Archontophoenix cunninghamiana leaves. Norway, Calophoma sandfjordenica from a piece of board on a rocky shoreline, Clavaria parvispora on soil, Didymella finnmarkica from a piece of Pinus sylvestris driftwood. Poland, Sugiyamaella trypani from soil. Portugal, Colletotrichum feijoicola from Acca sellowiana. Russia, Crepidotus tobolensis on Populus tremula debris, Entoloma ekaterinae, Entoloma erhardii and Suillus gastroflavus on soil, Nakazawaea ambrosiae from the galleries of Ips typographus under the bark of Picea abies. Slovenia, Pluteus ludwigii on twigs of broadleaved trees. South Africa, Anungitiomyces stellenboschiensis (incl. Anungitiomyces gen. nov.) and Niesslia stellenboschiana on Eucalyptus sp. leaves, Beltraniella pseudoportoricensis on Podocarpus falcatus leaf litter, Corynespora encephalarti on Encephalartos sp. leaves, Cytospora pavettae on Pavetta revoluta leaves, Helminthosporium erythrinicola on Erythrina humeana leaves, Helminthosporium syzygii on a Syzygium sp. bark canker, Libertasomyces aloeticus on Aloe sp. leaves, Penicillium lunae from Musa sp. fruit, Phyllosticta lauridiae on Lauridia tetragona leaves, Pseudotruncatella bolusanthi (incl. Pseudotruncatellaceae fam. nov.) and Dactylella bolusanthi on Bolusanthus speciosus leaves. Spain, Apenidiella foetida on submerged plant debris, Inocybe grammatoides on Quercus ilex subsp. ilex forest humus, Ossicaulis salomii on soil, Phialemonium guarroi from soil. Thailand, Pantospora chromolaenae on Chromolaena odorata leaves. Ukraine, Cadophora helianthi from Helianthus annuus stems. USA, Boletus pseudopinophilus on soil under slash pine, Botryotrichum foricae, Penicillium americanum and Penicillium minnesotense from air. Vietnam, Lycoperdon vietnamense on soil. Morphological and culture characteristics are supported by DNA barcodes.
... The new species occupies a basal position in the ITS phylogeny, which may support a dedicated genus. However, in addition to the LSU data, the gross morphology, anatomy, organoleptic features and ecology of the Balearic collections, fit well with the classic delineation of Ossicaulis (Holec & Kolařík 2013). With O. lachnopus, O. salomii shares the shape, but not the size, of the spores; with O. lignatilis, spore calibre but not the shape. ...
Coniochaeta dendrobiicola Sujit Shah, sp. nov.
Colour illustrations. Dendrobium longicornu orchid species from Chitlang
village, Makwanpur district, Nepal. Colony after 15 d on PDA, OA and CDA;
conidia, conidiogenous cells and hyphae. Scale bars = 10, 10 and 100 μm.
Sujit Shah, Bijaya Pant, Central Department of Botany, Tribhuvan University, Nepal; e-mail: b.pant@cdbtu.edu.np
Rohit Sharma & Yogesh S. Shouche, National Centre for Microbial Resource (NCMR), National Centre for Cell Science, India;
e-mail: rohit@nccs.res.in & yogesh@nccs.res.in
Jyotsna Sharma, Department of Plant and Soil Science, Texas Tech. University, USA; e-mail: jyotsna.sharma@ttu.edu
Etymology. Name reflects the host genus it was isolated from, Dendrobium
longicornu.
Classification — Coniochaetaceae, Coniochaetales, Sordariomycetes.
Vegetative hyphae thin, septate, smooth 1.2–2.4 μm wide.
Conidiogenous cells arising laterally from vegetative hyphae,
broader at base tapering towards apex (1.4 μm at base and
0.67 μm at apex). Conidia hyaline, smooth, cylindrical to allantoid,
variable in size, 4.35–11.28 × 1.2–2.3 μm. Sexual morph
absent which is reported in Coniochaeta velutina, C. prunicola,
C. africana isolated from Prunus (Damm et al. 2010, Weber
2002, Abdalla & Al-Rokibah 2003, Asgari & Zare 2006).
Cultural characteristics — Coniochaeta dendrobiicola was
first isolated on Czapek-Dox agar (CDA). The shape of the
colony was circular, with lemon yellow colour and pale regular
margin with pale white band as growing zone. The surface
was smooth with flat topography and submerged mycelium.
Colony 4 cm diam after 15 d of incubation, with 2–3 concentric
rings. On potato dextrose agar (PDA) the colony was circular
with regular margin, pale brown with yellowish margin having
radiating furrows. The surface was glistening, smooth with flat
topography and the presence of submerged mycelium. Colonies
reach 4 cm diam after 15 d of incubation, with 1–2 concentric
rings present. On oatmeal agar (OA) the colony shape was
circular with regular margin, lemon yellow with 1 cm thick white
growing margin. The colony surface was smooth, shiny with flat
topography and submerged mycelium. Colonies reach 4.5 cm
diam after 15 d of incubation, with a single concentric brown
ring present.
Habitat — Roots of Dendrobium longicornu, District Makwanpur,
Nepal.
Typus. Nepal, District Makwanpur, roots of Dendriobium lognicornu
(Orchidaceae), 25 May 2017, S. Shah (holotype culture and specimen,
MCC1811, preserved as metabolically inactive, ITS and LSU sequences
GenBank MK225602 and MK225603, MycoBank MB830652).
Notes — Phylogenetic trees of the ITS region was prepared
using sequences of C. dendrobiicola and other Coniochaeta
species obtained from GenBank. An NCBI BLASTn search
of ITS sequences showed closest similarity to be 93 % with
C. africana (CBS 120868, GenBank MH863095), 92 % with
C. velutina (STE-U 8315, GenBank KY312638), 92 % with
Coniochaeta angustispora (CBS 871.73, GenBank MH860816)
and 92 % with Coniochaeta nepalica (NBRC 30584, GenBank
LC146727).
... A key to the known species of Ossicaulis based on our data and publication by Holec and Kola rík (2013) is provided below. ...
Ossicaulis is a small genus in the family Lyophyllaceae. Two known species, O. lachnopus, and O. lignatilis, are distributed in north temperate regions. The third taxon, O. yunnanensis sp. nov., is described from the alpine belt of subtropics of southwestern China, and this genus is also reported for the first time from China. Morphologically, the new species is characterized by its whitish basidiomata and very small basidiospores. Molecular analyses from the nuclear rDNA internal transcribed spacer region (ITS) show that the subtropical alpine species is distinct from hitherto known Ossicaulis species and has a close relationship to O. lachnopus.
... D N A s t u d y. DNA from dried specimens (see Records and studied specimens) was isolated as described by Holec & Kolařík (2013). ITS-LSU rDNA was amplified using primers ITS1F and LR6, and the same primers, together with ITS4 and NL1, were used for sequencing (see Holec & Kolařík 2013 for details). ...
... DNA from dried specimens (see Records and studied specimens) was isolated as described by Holec & Kolařík (2013). ITS-LSU rDNA was amplified using primers ITS1F and LR6, and the same primers, together with ITS4 and NL1, were used for sequencing (see Holec & Kolařík 2013 for details). BlastN similarity search identified M. clavata as related to M. hiemalis and Hemimycena gracilis (Quél.) ...
The rare gilled fungus Mycena clavata is reported from the Czech Republic for the first time. It was found on large fallen trunks of Picea abies in Boubínský prales virgin forest, the best preserved montane old-growth forest in the country. The basidiomata occurred in the lower half of the trunks, either on their upper or lateral sides, on bark covered by mosses, on bare bark, or directly on wood covered by mosses, in wet times of the year. Descriptions and photographs of macro- and microcharacters are provided and data on the distribution and ecology of M. clavata are summarised. The species was sequenced for the first time. ITS-LSU rDNA sequences confirmed the species‘ distinct position and showed that M. clavata belongs to a moderately supported clade consisting of various Mycena and Hemimycena species, some of them recently transferred to the vaguely delimited genus Phloeomana.
... Isolation of DNA from dried specimens followed Holec and Kolařík (2013). ITS-LSU rDNA was amplified using primers ITS1F and NL4, and the same primers, together with ITS4 and NL1, were used for sequencing (see Holec and Kolařík 2013 for details). ...
... The final dataset comprised a total of 53 sequences (of which nine were obtained in this study) and 676 positions, of which 296 were variable and 93 were singletons. Bayesian phylogenetic analyses were carried out in MrBayes 3.0 (Ronquist and Huelsenbeck 2003) software using the same methods as described in Holec and Kolařík (2013). The evolutionary model, HKY ? ...
The holotype of Galerina saxicola Svrček, a never revised species described from the Czech Republic in 1994, was studied in detail. Due to its poor state and the impossibility to obtain DNA data, we fixed its taxonomic position by designating an epitype using recent material from the type locality. The species proved to be conspecific with Norwegian and Czech collections of Galerina stordalii A.H.Sm., both morphologically and molecularly. Full synonymy and diagnostic characters of G. stordalii are provided, its morphological and ecological variability is discussed, and information on type specimens is corrected. Our collections document that G. stordalii has a broader ecological amplitude than thought before, living not only on Sphagnum and peat in boreal and arctic-alpine habitats like bogs and snow beds, but also among mosses in boggy spruce forests, on decaying conifer trunks in old-growth forests and on moist sandstone rocks in a river canyon under the influence of climatic inversion.
