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Number of alleles, Gene diversity, Heterozigosity, and PIC Values of 34 rice accessions with 6 SSR markers in terms of aromatic gene

Number of alleles, Gene diversity, Heterozigosity, and PIC Values of 34 rice accessions with 6 SSR markers in terms of aromatic gene

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Rice plant (Oryza sativa L.) has various varieties. People generally prefers to rice with good taste and aroma. The aroma is part of the physicochemical properties of the rice plant. The aroma of rice arises because of the gene that controls the nature of the aroma. PCR (polymerase chain reaction) is an in vitro method for generating a large amount...

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... PCR results using the primers were then translated into digital values ( Table 2). Details of the PIC of the primers used can be seen in Table 3. Fragrance genes and badh2 genes were fragrance-encoding genes found in aromatic rice. ...

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Genetic relationship among 25 rice accessions have been evaluated using 21 SSR markers. A total of 87 alleles were produced by 21 polymorphic SSRs with an average of 4 alleles per SSRs. The SSR RM514 produced maximum number of alleles (9) followed by RM125 (7) and RM271 and RM447 (6). The PIC value varied from 0.07 (RM507) to 0.83 (RM514) with an average of 0.46. Jaccard’s dissimilarity coefficient ranged 0.10–0.89 with an average of 0.65. The highest genetic dissimilarity was observed between Dub Raj & IPB—1 (0.89), and Pant—12 & IPB—1 (0.89) and minimum dissimilarity observed between Kalanamak and CSR—10 (0.10). By evaluating data from clustering, dissimilarity and admixture nature the most diverse accessions were IPB—1, Pant—12, Dub Raj, Ram Raj, NDR 2064 and Sobhini. The accessions IPB—1, NDR—8002, BPT—5204, NDR—2065, PB—1, PUSA Basmati, and NDR—359 was found highly diverse as these accessions showed highest dissimilarity with respect to rest of the accessions. The NJ clustering pattern revealed 3 clusters and supported by 3 subpopulations determined from STRUCTURE analysis. The analysis of molecular variance (AMOVA) showed that 85% of allelic diversity was found within the population.