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Nodule number of 73 G. max and five G. soja (prefixed with ~) accessions with a significant difference in nodule number after inoculation with Bradyrhizobium japonicum strains USDA110 and USDA123 (P < 0.05), respectively. The accessions prefixed with an asterisk (*) were chosen to be inoculated with mixed USDA110 and USDA123 inoculum for the subsequent competition test

Nodule number of 73 G. max and five G. soja (prefixed with ~) accessions with a significant difference in nodule number after inoculation with Bradyrhizobium japonicum strains USDA110 and USDA123 (P < 0.05), respectively. The accessions prefixed with an asterisk (*) were chosen to be inoculated with mixed USDA110 and USDA123 inoculum for the subsequent competition test

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Background Symbiotic nitrogen fixation differs among Bradyrhizobium japonicum strains. Soybean inoculated with USDA123 has a lower yield than strains known to have high nitrogen fixation efficiency, such as USDA110. In the main soybean-producing area in the Midwest of the United States, USDA123 has a high nodule incidence in field-grown soybean and...

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