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Neoophiosphaerella sasicola. A, B. Ascomata on the natural host surface; C. Ascoma in longitudinal section; D. Ascomatal wall; E. Pseudoparaphyses; F. Ascus; G. Ascospore (arrowheads indicate gelatinous sheath; in India ink). All from KT 1706. Scale bars: A = 1 mm; B = 500 μm; C = 100 μm; D–G = 10 μm.
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We here taxonomically revise the suborder Massarineae (Pleosporales, Dothideomycetes, Ascomycota). Sequences of SSU and LSU nrDNA and the translation elongation factor 1-alpha gene (tef1) are newly obtained from 106 Massarineae taxa that are phylogenetically analysed along with published sequences of 131 taxa in this suborder retrieved from GenBank...
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As part of a series of papers dealing with the diversity of anamorphic saprophytic fungi in cloud forest remnants from the Veracruz state, an account of 25 species are described and illustrated. All of them but Helminthosporium solani are new records for the Mexican mycobiota. The species Dictyochaeta tumidospora, Fusariella intermedia, Hyphodiscos...
Citations
... Initially, based on morphological characters with basauxic conidiogenesis, Spegazzinia was accommodated in Apiosporaceae, Sordariomycetes (Hyde et al. 1998). However, Tanaka et al. (2015) transferred Spegazzinia to Didymosphaeriaceae (Dothideomycetes) based on molecular data. Morphologically, species of Spegazzinia have a distinctive conidiophore ontogeny, as well as two types of conidia: α conidia are composed of 4-8 subglobose, dark cells with long spines, while β conidia are generally subspherical or broadly ellipsoid, flattened in one plane, cruciately septate or muriform, pale brown and smooth-walled (Samarakoon et al. 2020). ...
Zhujiangyuan Nature Reserve, located in Qujing City, Yunnan Province, China, is reported with high fauna and floral diversity, while the fungal diversity of the region is poorly documented. During the summer season in 2023, decaying wood-inhabiting microfungi were collected from different microhabitats. The novel species were identified based on morphological characteristics and phylogenetic analyses (based on combined datasets of ITS, LSU, SSU, tef 1-α, and rpb 2 regions). Two species belong to Dothideomycetes ( viz. , Spegazzinia zhujiangyuanensis sp. nov. and Phaeoseptum zhujiangyuanense sp. nov. in Pleosporales) while the other one resides in Sordariomycetes ( Synnemasporella fanii sp. nov. in Diaporthales). The results are in conformity with the earlier studies that predicted higher fungal diversity in this region.
... Based on ITS, LSU, SSU and tef1-α sequence comparison with the GenBank database, similar species in Dictyosporiaceae were found. The sequences of 37 relevant species according to the blasting result and recent publications (Tanaka and Harada 2003;Chen et al. 2014;Tanaka et al. 2015;Boonmee et al. 2016;Liu et al. 2017;Yang et al. 2018a;Chen et al. 2020) were chosen for phylogenetic analyses (Table 1) and were downloaded from GenBank. Four gene regions (ITS, LSU, SSU and tef1-α) were individually aligned using the online service of MAFFT v. 7 (Madeira et al. 2019) and concatenated using PhyloSuite v. 1.2.2 . ...
The rotting wood in freshwater is a unique eco-environment favoring various fungi. During our investigation of freshwater fungi on decaying wood, three hyphomycetes were collected from Jiangxi and Guangxi Provinces, China. Based on the morphological observations and phylogenetic analysis of a combined DNA data containing ITS, LSU, SSU and tef1-α sequences, two new Trichobotrys species, T. meilingensis and T. yunjushanensis , as well as a new record of T. effusa , were introduced. Additionally, a comprehensive description of the genus with both morphological and molecular data was first provided.
... The Didymosphaeriaceae represents a well-supported clade within the Massarineae (Tanaka et al. 2015;Yuan et al. 2020). Ariyawansa et al. (2014) synonymized Montagnulaceae under Didymosphaeriaceae, accommodating 39 genera including our new genus Agrorhizomycota (Wijayawardene et al. 2014(Wijayawardene et al. , 2022Liu et al. 2022Tanaka et al. 2015Wanasinghe et al. 2016;Yuan et al. 2020, Ren et al. 2022). ...
... The Didymosphaeriaceae represents a well-supported clade within the Massarineae (Tanaka et al. 2015;Yuan et al. 2020). Ariyawansa et al. (2014) synonymized Montagnulaceae under Didymosphaeriaceae, accommodating 39 genera including our new genus Agrorhizomycota (Wijayawardene et al. 2014(Wijayawardene et al. , 2022Liu et al. 2022Tanaka et al. 2015Wanasinghe et al. 2016;Yuan et al. 2020, Ren et al. 2022). More than two third of the genera in the family were introduced based on their sexual morphs. ...
... Micromorphology supports its classification, in the Apiosporaceae (Sordariomycetes) (Samarakoon et al. 2020a). Based on a multi-locus phylogeny using sequences of S. deightonii and S. tessarthra, Tanaka et al. (2015) placed Spegazzinia in Didymosphaeriaceae (Dothideomycetes). ...
In this study, we investigated two distinct new phylogenetic lineages of root-colonizing dark septate endophytic fungi colonizing wheat ( Triticum aestivum ) roots from a long-term agricultural experimental site in Hungary. According to four-locus (internal transcribed spacer, partial large and small subunit regions of nuclear ribosomal DNA, and partial translation elongation factor 1-alpha) phylogenetic analyses, the isolates belong to the Lentitheciaceae and Didymosphaeriaceae of the Pleosporales ( Dothideomycetes ). We studied the morphology and culture characteristics of the strains. We carried out in vitro resynthesis pot experiments with their original hosts and found no overall negative effect of the inoculation with different isolates of the new taxa. One of the lineages belonged to the genus Poaceascoma ( Lentitheciaceae ) and represented a novel species described here as Poaceascoma zborayi . We could describe conidia-like structures from this species. Isolates of the other lineage represented a monotypic novel genus in the Didymosphaeriaceae . Accordingly, the new genus, Agrorhizomyces , represented by the species A. patris , is introduced. Sterile, globose structures resembling immature sporocarps were detected. Sequence similarity searches indicated that P. zborayi might be widely distributed, while no sequence similar to A. patris was found outside the sampling area.
... The effects of endophytic fungi on the morphological parameters of cucumber are shown in Fig. 4. Most of the growth parameters evaluated in cucumber improved by both when compared with those of the experimental control, with As reported in the literature (Elis 1971;Tanaka et al. 2015;Hongsanan et al. 2020), most Periconia species are mainly known from their asexual morphs, characterized by conidiophores that are macronematous, mononematous, branched or unbranched, and pale to dark brown. Conidiogenous cells are discrete on the terminal or intercalary of the stipe and are monoblastic to polyblastic (Markovskaja and Kačergius 2014;Chuaseeharonnachai et al. 2016;Su et al. 2023). ...
... Particularly, the order Pleosporales, which represents the largest order of Dothideomycetes (Zhang et al. 2012), comprises species commonly identified as dark septate endophytes (DSE) of Class 4 endophytes as defined by Rodriguez et al. (2009). P. macrospinosa belongs to the Periconiaceae (Pleosporales, Pleosporomycetidae) (Tanaka et al. 2015) and is one of the most common DSE species (Grünig et al. 2011;Mandyam 2008Mandyam , 2010Fors et al. 2020). Several factors contribute to the prevalence and success of ascomycetes as endophytes in different plant-microbe interactions. ...
Cucurbits are subject to a variety of stresses that limit their sustainable production, despite their important role in ensuring food security and nutrition. Plant stress tolerance can be enhanced through fungal endophytes. In this study, two endophytes isolated from wild plant roots, were tested to determine their effect on the growth promotion of cucumber (Cucumis sativus L.) plants. The phylogenetic analysis revealed that the designated isolates were Aspergillus elegans and Periconia macrospinosa. The results of the Plant Growth Promoting Fungal (PGPF) tests showed that both Aspergillus elegans and Periconia macrospinosa have a zinc solubilizing capacity, especially A. elegans, with a solubilization index higher than 80%. Also, both have a high salt tolerance (10–15% NaCl for P. macrospinosa and A. elegans, respectively), cellulolytic activity, and inhibition indices of 40–64.53%. A. elegans and P. macrospinosa had antagonistic effects against the cucumber phytopathogenic fungi Verticillium dahliae and Fusarium oxysporum, respectively. However, A. elegans and P. macrospinosa didn’t exhibit certain potential plant benefits, such as the production of hydrogen cyanide (HCN) and phosphate solubilization. The chlorophyll content and growth parameters of two-month-old cucumber plants inoculated with the fungal species were significantly better than those of the controls (non-inoculated); the shoot dry weights of inoculated plants were increased by 138% and 170% for A. elegans and P. macrospinosa, respectively; and the root colonization by fungal endophytes has also been demonstrated. In addition to the fact that P. macrospinosa has long been known as PGPF, this is the first time that the ability of A. elegans to modulate host plant growth has been demonstrated, with the potential to be used as a biofertilizer in sustainable agriculture.
... Newly generated sequences were subjected to BLASTn searches at NCBI (https://blast.ncbi. nlm.nih.gov/Blast.cgi) to recognize highly similar sequences in the related literature (Brahmanage et al. 2020, Tanaka et al. 2015) ( Table 2). Related sequences and newly generated data were aligned in MAFFT 6.864b online tool with the FFT-NS-i method (Katoh et al. 2019). ...
... Our isolate is characterized by having globose pycnidial conidiomata, thick peridial wall composed of textura angularis and textura prismatica cell types, conidiophores reduced to conidiogenous cells with percurrent proliferation from the inner layer of the pycnidial wall. Conidiogenesis cells are doliiform, terminal, and holoblastic producing hyaline, cylindrical to ellipsoidal, straight or slightly curved and guttulate conidia (Li et al. 2016, Quaedvlieg et al. 2013, Tanaka et al. 2015. The conidiomata diameter of S. samroiyotensis is larger (170-250 μm) than S. uniseptata (150 μm), and S. samroiyotensis has a higher L/ W ratio for the conidia (S. samroiyotensis = 8.25, S. uniseptata = 3.67). ...
... Most Stagonospora isolates are reported from Cyperaceae, Poaceae, and Typhaceae plants (Quaedvlieg et al. 2013, Thambugala et al. 2017. Species of this genus have been recorded from different latitudes in both northern and southern hemispheres (Tanaka et al. 2015), as well as in tropical countries such as Thailand and temperate countries including Italy (Quaedvlieg et al. 2013). Stagonospora species have been recorded in a diverse range of grass lands to wetlands habitats (Brahmanage et al. 2020, Calabon et al. 2021, Quaedvlieg et al. 2013, Li et al. 2016, Thambugala et al. 2017. ...
Stagonospora is an asexual morph genus that is classified under Massarinaceae in Dothideomycetes. Stagonospora species have been recorded in both tropical and temperate regions. Several species have been reported as saprobic or opportunistic pathogenic lifestyles on grasses and grass-like plants. In this study, Stagonospora was collected from a Typha species in Sam Roi Yot wetland in central Thailand. Morphological examination was coupled with multi-loci phylogenetic analyses using maximum likelihood and Bayesian interference of a data set containing large subunit ribosomal rDNA (LSU rDNA), internal transcribed spacer (ITS) regions, small subunit ribosomal rDNA (SSU rDNA), and translation elongation factor 1-alpha (tef1-α) sequences. Our new taxon is distinguishable from other Stagonospora species by having hyaline, one-septate conidia that taper toward the base. This discovery holds significant value in comprehending the fungal diversity within Thailand's wetlands and the specific fungal communities linked to Typha plants.
... Pleosporalean species are cosmopolitan and ubiquitous in diverse ecological niches. Their life modes include epiphytes, endophytes or parasites on living organisms, hyperparasites on fungi or insects, saprobes, pathogens and lichenised fungi (Zhang et al. 2012;Hyde et al. 2013;Tanaka et al. 2015;Jaklitsch et al. 2016a;Hongsanan et al. 2020). Of these, several genera, such as Alternaria, Bipolaris, Didymella, Leptospharia, Parastagonospora, Phaeosphaeria and Pyrenophora, have been reported as plant pathogens causing severe diseases on economic crops (Quaedvlieg et al. 2013;Woudenberg et al. 2013Woudenberg et al. , 2014Woudenberg et al. , 2015Ariyawansa et al. 2015a, b;Chen et al. 2015Chen et al. , 2017Tanaka et al. 2015;El-Demerdash 2018;Khiralla et al. 2019;Bhunjun et al. 2020;Hongsanan et al. 2020;Backes et al. 2021;Bartosiak et al. 2021;Li et al. 2023). ...
... Their life modes include epiphytes, endophytes or parasites on living organisms, hyperparasites on fungi or insects, saprobes, pathogens and lichenised fungi (Zhang et al. 2012;Hyde et al. 2013;Tanaka et al. 2015;Jaklitsch et al. 2016a;Hongsanan et al. 2020). Of these, several genera, such as Alternaria, Bipolaris, Didymella, Leptospharia, Parastagonospora, Phaeosphaeria and Pyrenophora, have been reported as plant pathogens causing severe diseases on economic crops (Quaedvlieg et al. 2013;Woudenberg et al. 2013Woudenberg et al. , 2014Woudenberg et al. , 2015Ariyawansa et al. 2015a, b;Chen et al. 2015Chen et al. , 2017Tanaka et al. 2015;El-Demerdash 2018;Khiralla et al. 2019;Bhunjun et al. 2020;Hongsanan et al. 2020;Backes et al. 2021;Bartosiak et al. 2021;Li et al. 2023). ...
... A comprehensive study of the genera in Pleosporales was carried out by Zhang et al. (2012), based on morphological studies of the type specimens coupled with phylogenetic analyses. Consequently, the taxonomic treatment of numerous Pleosporales was updated by various authors, based on polyphasic taxonomic approaches, mainly using morphology-phylogeny-based taxonomy (Ariyawansa et al. 2014(Ariyawansa et al. , 2015aPhookamsak et al. 2014Phookamsak et al. , 2015Tanaka et al. 2015;Thambugala et al. 2015;Boonmee et al. 2016;Jaklitsch and Voglmayr 2016;Jaklitsch et al. 2016aJaklitsch et al. , b, 2018Su et al. 2016;Chen et al. 2017;Hashimoto et al. 2017;Wanasinghe et al. 2017a, b). Even though novel taxa of Pleosporales have been dramatically increasing over the last ten years after the taxonomic circumscription provided by Zhang et al. (2012) and Hyde et al. (2013), there is still over a quarter of the total known species lacking molecular data and/or reliable phylogenetic markers for clarifying the placements in Pleosporales. ...
This article presents the results of an ongoing inventory of Ascomycota in Yunnan, China, carried out as part of the research project series “Exploring ascomycete diversity in Yunnan”. From over 100 samples collected from diverse host substrates, microfungi have been isolated, identified and are currently being documented. The primary objective of this research is to promote the discovery of novel taxa and explore the ascomycete diversity in the region, utilising a morphology-phylogeny approach. This article represents the second series of species descriptions for the project and introduces three undocumented species found in the families Bambusicolaceae, Dictyosporiaceae and Periconiaceae, belonging to the suborder Massarineae (Pleosporales, Dothideomycetes). These novel taxa exhibit typical morphological characteristics of Bambusicola, Periconia and Trichobotrys, leading to their designation as Bambusicola hongheensis, Periconia kunmingensis and Trichobotrys sinensis. Comprehensive multigene phylogenetic analyses were conducted to validate the novelty of these species. The results revealed well-defined clades that are clearly distinct from other related species, providing robust support for their placement within their respective families. Notably, this study unveils the phylogenetic affinity of Trichobotrys within Dictyosporiaceae for the first time. Additionally, the synanamorphism for the genus Trichobotrys is also reported for the first time. Detailed descriptions, illustrations and updated phylogenies of the novel species are provided, and thus presenting a valuable resource for researchers and mycologists interested in the diversity of ascomycetes in Yunnan. By enhancing our understanding of the Ascomycota diversity in this region, this research contributes to the broader field of fungal taxonomy and their phylogenetic understanding.
... The introduction of novel genera has been significantly increased in the past two decades through extensive sampling and molecular phylogenetic analyses [1,[3][4][5][6][7]. Currently, 20 genera are accepted within the family [8], with the majority being recognized as asexual morphs and only 5 genera having been documented to possess sexual morphs, viz., Dictyosporium, Gregarithecium, Immotthia, Pseudocoleophoma, and Verrucoccum [9][10][11][12][13]. ...
... Dictyocheirospora submersa can be easily distinguished from Dictyoc. pseudomusae by the absence of globose to subglobose, hyaline appendages growing from the apical cells or side of the outer rows [10]. The conidia of Dictyoc. ...
... at room temperature (24-27 °C) in natural light after 15 days, irregular, rough, dry, fluffy, and dense in the middle; margin sparse and wavy, white and orange-brown; and the reverse orange-brown in the middle and pale orange-brown at the margin. [3,10,41,42]. Therefore, ZHKUCC 24-0004 is identified as Dictyos. ...
Over the past two decades, numerous novel species have been identified within Dictyosporiaceae, primarily in Dictyocheirospora and Dictyosporium. A recent monograph has revealed that these two genera exhibit a distinct preference for freshwater habitats, particularly in southern China. However, further investigation into the distribution and diversity of the two genera in Guangdong and Guizhou Provinces remains insufficient. In this study, we conducted an analysis of four intriguing cheiroid hyphomycetes collected from flowing rivers in these two regions. Through morphological and phylogenetic analyses incorporating combined LSU, SSU, ITS, and tef1-α sequence data, we have identified them as a novel species in Dictyocheirospora (Dictyoc. submersa sp. nov.), two novel species in Dictyosporium (Dictyos. guangdongense sp. nov. and Dictyos. variabilisporum sp. nov.), and one previously documented species (Dictyos. digitatum). Specifically, the identification of Dictyos. guangdongense is primarily based on its distinct morphology, characterized by complanate, cheiroid, and brown to dark brown conidia, with a hyaline, short, and atrophied appendage arising from the apical cell of the outer row. In addition, the morphological distinctions between Dictyocheirospora and Dictyosporium are further clarified based on our new data. This study also highlights a few phylogenetic matters regarding Dictyosporiaceae.
... Periconia was previously classified under Massarinaceae [10]. Subsequently, Tanaka et al. [11] reclassified Periconia in Periconiaceae based on the multi-locus phylogenetic analysis, which showed that Periconia species formed a distinct cluster separate from Massarinaceae. Until now, the latest treatment of Periconia by Tanaka et al. [11] has been followed by subsequent studies [12][13][14][15][16][17]. ...
... Subsequently, Tanaka et al. [11] reclassified Periconia in Periconiaceae based on the multi-locus phylogenetic analysis, which showed that Periconia species formed a distinct cluster separate from Massarinaceae. Until now, the latest treatment of Periconia by Tanaka et al. [11] has been followed by subsequent studies [12][13][14][15][16][17]. Most Periconia species have been reported based on their asexual morphs, while five species have been reported based on their sexual morphs, viz., P. didymosporum, P. homothallica, P. igniaria, P. prolifica, and P. pseudodigitata [11,17]. ...
... Until now, the latest treatment of Periconia by Tanaka et al. [11] has been followed by subsequent studies [12][13][14][15][16][17]. Most Periconia species have been reported based on their asexual morphs, while five species have been reported based on their sexual morphs, viz., P. didymosporum, P. homothallica, P. igniaria, P. prolifica, and P. pseudodigitata [11,17]. The asexual morph is mainly characterized by macronematous, mononematous, brown, branched, or unbranched conidiophores, with brown, ovoid to clavate or spherical conidial heads, intercalary or terminal conidiogenous cells with catenate or solitary, golden brown to dark brown, globose to subglobose, aseptate, smooth or verruculose conidia [11,15,[18][19][20][21]. ...
During a survey of fungi on Wurfbainia villosa in Guangdong Province, China, three novel species, Periconia endophytica, P. yangjiangensis, and P. wurfbainiae, belonging to Periconiaceae in Pleosporales, Dothideomycetes are proposed based on morphological and phylogenetic evidence. Periconia endophytica was isolated from the healthy leaves of W. villosa, while P. yangjiangensis and P. wurfbainiae were obtained from the dead stems of the same host. Notably, holomorphs were observed in P. wurfbainiae. The morphological characteristics of the novel taxa are compared with closely related species within Periconia. Illustrations, morphological descriptions, and phylogenetic analyses are provided for the novel taxa. Multilocus phylogeny of the combined internal transcribed spacer (ITS), large subunit nuclear rDNA (LSU), small subunit nuclear ribosomal rDNA (SSU), and partial translation elongation factor 1–α (tef1-α) regions supported the establishment of three new species. Furthermore, the taxa clustering in Periconia, Flavomyces fulophazii, and Sporidesmium tengii, are discussed for further investigation of their taxonomic placements.
... However, only five species have been described from their sexual morphs: P. didymosporum, P. homothallica, P. igniaria, P. prolifica, and P. pseudodigitata. The sexual morphs of Periconia are characterised by scattered or clustered, globose ascomata with a central ostiole, 8-spored asci with the ocular chamber, and broadly fusiform, 1-septate, hyaline, smooth ascospores with a sheath (Tanaka et al. 2015;Yang et al. 2022). ...
... Distribution. China, Japan, Lithuania, Netherlands, Thailand (Markovskaja and Kačergius 2014;Tanaka et al. 2015;Jayasiri et al. 2019;Tennakoon et al. 2021;Yang et al. 2022;Su et al. 2023). ...
... Substratum. Saprobic on Ampelopsis brevipedunculata (Vitaceae) (Tanaka et al. 2015), Angelica sylvestris (Apiaceae) (Markovskaja and Kačergius 2014), Benthamidia japonica (Cornaceae) (Tanaka et al. 2015), Celtis formosana (Cannabaceae) (Tennakoon et al. 2021), Conium maculatum (Apiaceae) (Markovskaja and Kačergius 2014), Cananga odorata (Annonaceae) (Wang et al. 2008), Ficus altissima, F. virens and F. benjamina (Moraceae) (de Silva et al. 2022), Heracleum sosnowskyi (Apiaceae) (Markovskaja and Kačergius 2014), Macaranga tanarius (Euphorbiaceae) (Tennakoon et al. 2021), Magnolia grandiflora (Magnoliaceae) (Jayasiri et al. 2019), Peltophorum sp. (Fabaceae) (Jayasiri et al. 2019), Prunus armeniaca (Rosaceae) (Yang et al. 2022), Prunus verecunda (Rosaceae) (Tanaka et al. 2015), Imperata cylindrical (Poaceae) (Su et al. 2023 Notes. ...
... Previous studies have highlighted the varied classification of Bactrodesmium species across different classes within the Ascomycota (Réblová et al. 2020). Majority of Bactrodesmium species are located within Sordariomycetes, while B. cubense and B. gabretae are classified under Dothideomycetes and Leotiomycetes, respectively (Koukol & Kolárová, 2010;Tanaka et al. 2015). Classification of bactrodesmium-like species requires multigene phylogenetic analyses since it is difficult to identify this taxon based on morphological evidence. ...
In this study, four terrestrial collections of bactrodesmium-like hyphomycetous fungi were isolated from rotten wood in Guizhou Province, China. Phylogenetic analyses based on the combined LSU, ITS, mtSSU, and SSU sequence matrix indicate that these four isolates represent one species belonging to the Sclerococcum within the Dactylosporaceae, and present a distinct lineage. Therefore, Sclerococcum pseudobactrodesmium sp. nov. was introduced with corresponding morphological descriptions. Sclerococcum pseudobactrodesmium represents the first lignicolous asexual species from a terrestrial habitat.
