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Neohelicosporium bambusicola (MFLU 21-0189, holotype). (a,b) Colony on culms of bamboo; (c,e-g,i) conidiogenous cells and conidia; (d) conidiophores; (h,k,l) conidia; (j) germinated conidium; (m,n) colony cultures on PDA (observe and reverse). Scale bars: (a) = 500 μm, (b) = 200 μm, (e,f,k,l) = 20 μm, (c,d,g-j) = 10 μm.
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Tubeufiaceae, a cosmopolitan family with a worldwide distribution, is mostly reported as saprobic on decaying woody materials from both aquatic and terrestrial habitats. The family is commonly found as helicosporous hyphomycetes, while some are chlamydosporous and phragmosporous. In this study, thirteen helicosporous hyphomycetes were collected fro...
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Citations
... The conidial morphology of most Neohelicomyces species closely resembles those of Helicomyces and the typical helicoid Tubeufia genera (Zhao et al. 2007;Luo et al. 2017;Lu et al. 2018b;Ma et al. 2023b). However, most Neohelicomyces species can easily be distinguished by their longer, hyphae-like and conspicuous conidiophores, when compared to those of Helicomyces and Tubeufia (Morgan 1892;Linder 1929;Rao and Rao 1964;Goos 1985;Zhao et al. 2007;Hyde et al. 2016;Lu et al. 2017bLu et al. , 2018bLu et al. , 2023bKuo and Goh 2018;Tian et al. 2022;Ma et al. 2023b). Only two species, Neohelicomyces longisetosus and N. thailandicus, exhibit morphological variations in conidiophores when compared to other Neohelicomyces species. ...
Neohelicomyces species are a group of helicosporous hyphomycetes with the potential to produce secondary metabolites. During our investigation of helicosporous fungi, six collections were isolated from both terrestrial and freshwater habitats in Guizhou Province, China. Based on multigene phylogenetic analysis (ITS, LSU, tef1α and rpb2), coupled with morphological data, three new Neohelicomyces species, viz. N. guizhouensis, N. helicosporus and N. hydei were established. A list of accepted Neohelicomyces species with molecular data was provided. The strain of Neohelicomyces pallidus (UAMH 10535) was synonymised under N. denticulatus based on molecular data.
... The amplification conditions were set as follows: denaturation at 95 • C for 4 min, 35 cycles of 30 s at 94 • C, 40 s at 53 • C, 1 min at 72 • C, a final extension of 8 min at 72 • C, and then coolant at 14 • C [35,36]. In this study, the internal transcribed spacer (ITS) regions were amplified with the primers ITS1F/ITS4 [30,34,37,38]. Some full lengths of ITS sequences that failed to be amplified were divided into two parts for amplification with primer pairs ITS1F/5.8S and 5.8SR/ITS4, respectively [39], and then sequences of these two parts were spliced together to obtain the full length of the ITS sequence. ...
Species of the genus Thelephora (Thelephorales, Thelephoraceae) are ectomycorrhizal symbionts of coniferous and broad-leaved plants, and some of them are well-known edible mushrooms, making it an exceptionally important group ecologically and economically. However, the diversity of the species from China has not been fully elucidated. In this study, we conducted a phylogenetic analysis based on the internal transcribed spacer (ITS) regions, using Maximum Likelihood and Bayesian analyses, along with morphological observations of this genus. Four new species from China are proposed, viz., T. dactyliophora, T. lacunosa, T. petaloides, and T. pinnatifida. In addition, T. sikkimensis originally described from India is reported for the first time from China. Thelephora dactyliophora, T. pinnatifida, and T. sikkimensis are distributed in subtropical forests and mainly associated with plants of the families Fagaceae and Pinaceae. Thelephora lacunosa and T. petaloides are distributed in tropical to subtropical forests. Thelephora lacunosa is mainly associated with plants of the families Fagaceae and Pinaceae, while T. petaloides is mainly associated with plants of the family Fagaceae. Line drawings of microstructures, color pictures of fresh basidiomes, and detailed descriptions of these five species are provided.
... A megablast search using the newly obtained sequences was performed to find the closely related taxa in the GenBank nucleotide database. The combined alignments of ITS, LSu, tef 1-α, and rpb2 sequences data from the closest relatives in Tubeufiaceae were used to generate phylogenetic trees following Li et al. (2022) and Tian et al. (2022). The consensus and reference sequences were aligned with MAFFT version 7.0 (http://mafft.cbrc.jp/ ...
... In this study, two Tubeufiaceae species were collected from terrestrial habitats in Xizang Autonomous region, China (Figures 1-3). Numerous novel helicosporous taxa have recently been discovered in subtropical to tropical terrestrial and freshwater habitats (Lu et al. 2018b, Phookamsak et al. 2018, dong et al. 2020, Li et al. 2022, Tian et al. 2022. ...
... Li et al. (2022) reported two new genera and nine new species of Tubeufiaceae from northwestern Yunnan province from freshwater habitats. Tian et al. (2022) reported the first geographical record of T. longihelicospora from China. Ma et al. (2023) introduced Pseudotubeufia, and Xiao et al. (2023) reported three novel Helicosporium species from terrestrial and freshwater habitats. ...
Tubeufiaceae species have diverse morphologies and habitats and are distributed in both tropical and temperate regions. In the past decade, several new and interesting Tubeufiaceae species were reported from China in aquatic and terrestrial habitats. In this study, we investigated two saprobic species collected on decaying wood in a terrestrial habitat of Xizang Autonomous Region, China. Through phylogenetic analyses of a combined ITS, LSU, tef1-α, and rpb2 dataset coupled with detailed morphological examinations, a novel species within Neomanoharachariella was identified. Phylogenetic analyses revealed that this new species formed a sister clade to Neomanoharachariella aquatica, but can be morphologically distinguished by its pale brown, septate, and flexuous conidiophores, alongside dark brown setae that turn hyaline at the apex and distinct muriform conidia. In addition, this study presents the first asexual and geographical record of Acanthostigmina (A. multiseptatum) from China. Acanthostigmina multiseptatum is characterized by micronematous, hyaline, and septate conidiophores with unique pleurogenous and helicoid conidia.
... anceps and T. coronata). Tubeufia species are widely distributed in tropical and temperate regions, including freshwater and terrestrial habitats, occurring primarily on well-rotted wood (Barr 1979;Rossman 1987;Ho et al. 2002;Tsui and Berbee 2006;Tsui et al. 2007;Zhao et al. 2007;Boonmee et al. 2011Boonmee et al. , 2014Chaiwan et al. 2017;Dai et al. 2017;Doilom et al. 2017;Lu et al. 2017Lu et al. , 2018aLu et al. , 2018bLu et al. , 2022Lu et al. , 2023Tian et al. 2022). There are currently 56 accepted species in Tubeufia, as listed in Table 1. ...
... Morphologically, Tubeufia guttulata differs from T. cocois in that it has longer conidiophores (101-247 μm vs. 38-123 μm) and larger conidia (170-220 μm vs. 116-136 μm). In addition, the helicoid conidia of T. guttulata become uncoiled in water, while T. cocois are coiled (Tian et al. 2022). Etymology. ...
... Notes. Tian et al. (2022) introduced Tubeufia cocois from the dead leaves of Cocos nucifera in Thailand. Our newly isolated strain (GZCC 22-2038) clusters with T. cocois (MFLUCC 22-0001) with 93% ML/1.00 PP support. ...
During an investigation of helicosporous fungi in China, a total of seven helicosporous hyphomycetes were obtained from decaying wood in the southern region of the country. Based on phylogenetic analyses using a combined LSU, ITS, tef1α , and rpb2 sequence matrix, in conjunction with morphological comparisons, these taxa were classified within Tubeufia (Tubeufiaceae, Tubeufiales) and were recognized as three new species, viz. Tubeufia guttulata , T. hainanensis , and T. muriformis , as well as one new distribution record, viz. T. cocois . Evidence for these new taxa and the new record, descriptions, illustrations, notes, and phylogenetic evidence are provided for the newly collected helicosporous species.
... Due to their ability to produce active secondary metabolites with distinct structures, these fungi have attracted scientists' interest and become a popular study area. Numerous novel helicosporous taxa have recently been discovered in subtropical to tropical terrestrial and freshwater habitats [9][10][11][12]21,22,[30][31][32]. In addition, a growing number of active secondary metabolites have been isolated from helicosporous fungi [33][34][35][36]. ...
Helicosporous hyphomycetes is a group of filamentous fungi that shows promising application prospects in metabolizing bioactive natural compounds. During a study of helicosporous fungi in China, six new helicosporous taxa were collected and isolated from decaying wood in Guangxi Zhuang Autonomous Region, China. Morphological comparisons with multi-gene phylogenetic analyses revealed that these six taxa belong to Helicosporium (Tubeufiaceae, Tubeufiales), and they were recognized as three novel species and were named Helicosporium liuzhouense, H. multidentatum, and H. nanningense. Detailed descriptions and illustrations of the newly discovered taxa and comparisons with similar fungi are provided. In addition, a list and a key to accepted Helicosporium species are provided.
... Helicosporous fungi are filamentous fungi whose conidia curve through at least 180 degrees in one plane or three-dimensional space as they grow longer [2,3,[9][10][11][27][28][29][30] Due to their ability to produce active secondary metabolites with distinct structures, these fungi have attracted scientists' interest and become a popular study area. Numerous novel helicosporous taxa have recently been discovered in subtropical to tropical terrestrial and freshwater habitats [9][10][11][12]21,22,[30][31][32]. In addition, a growing number of active secondary metabolites have been isolated from helicosporous fungi [33][34][35][36]. ...
Helicosporous hyphomycetes is a class of filamentous fungi that shows promising application prospects in metabolizing bioactive natural compounds. During a study of helicosporous fungi in China, six new helicosporous taxa were collected and isolated from decaying wood in Guangxi Zhuang Autonomous Region, China. Morphological comparisons with multi-gene phylogenetic analyses revealed that these six taxa belong to Helicosporium (Tubeufiaceae, Tubeufiales), and they can be recognized as three novel species, named Helicosporium liuzhouense, H. multidentatum and H. nanningense. Detailed descriptions and illustrations of the newly discovered taxa and comparisons with similar fungi are provided. In addition, a list and a key to accepted Helicosporium species are provided.
... The 99.4 g of crude extract was subjected to column chromatography (CC) over silica gel (PE/EtOAc, v/v, 100:0→0:100) to give 20 fractions (Fr. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. Of these, Fr.12 was separated with Sephadex LH-20 in MeOH to yield 4 subfractions (Fr.12.1-12.4). ...
From the rice fermentation product of a new record fungus, Neohelicosporium griseum, two new polyketides, neogrisphenol A (1) and neogrisphenol B (2), one new isochroman-1-one, (S)-6-hydroxy-7-methoxy-3,5-dimethylisochroman-1-one (3), and four known compounds (4–7) were isolated. Their structures were determined using 1D- and 2D-NMR, mass spectrometry, and chemical calculations. The C-3~C-2′ polymerization mode between the two α-naphthalenone derivative moieties is uncommon in compounds 1 and 2. Meanwhile, compounds 1–2 and 5 exhibited antibacterial activity against Bacillus subtilis, Clostridium perfringens, Staphylococcus aureus, and Staphylococcus aureus, with MIC values ranging between 16 and 31 µg/mL. In addition, compound 5 showed antifungal activity against Sclerotinia sclerotiorum and Phytophthora nicotianae var. nicotianae, with respective IC50 values of 88.14 ± 2.21 µg/mL and 52.36 ± 1.38 µg/mL. Compound 1 showed significant cytotoxicity against A2780, PC-3, and MBA-MD-231 cell lines with respective IC50 values of 3.20, 10.68, and 16.30 µM, and the cytotoxicity against A2780 cells was even higher than that of cisplatin (CDDP). With an IC50 value of 10.13 µM, compound 2 also exhibited cytotoxicity against A2780. The in vitro results showed that compound 1 inhibited A2780 cell proliferation, induced apoptosis, and arrested the cell cycle at the S-phase in a concentration-dependent manner.
... Hsieh et al. (2021) established Parahelicomyces to replace Pseudohelicomyces and transferred all species of Pseudohelicomyces to Parahelicomyces. Until recently, nine species are accepted in Parahelicomyces (Lu et al., 2018b;Li et al., 2022;Tian et al., 2022). Tubeufia is the largest genus in Tubeufiaceae and is commonly reported as saprobes on submerged decaying wood in freshwater habitats (Ho et al., 2001;Cai et al., 2002;Liu et al., 2018;Lu et al., 2018b;Jayasiri et al., 2019). ...
The diversity of lignicolous freshwater fungi in northwestern Yunnan, China, has been studied for several years in the College of Agriculture and Biological Science, at Dali University. Over the last 5 years, we published two new genera and nine new species of Tubeufiaceae from northwestern Yunnan. This study focused on introducing tubeufia-like hyphomycetous fungi found in freshwater lakes in the northwestern Yunnan plateau. Eleven fresh collections of tubeufiaceous taxa were gathered and identified. Among them, a new genus, Neomanoharachariella , is introduced to accommodate Neomanoharachariella aquatica , which is characterized by a light brown to dark brown color, dictyoseptate, and broadly oval to ellipsoid and well-developed conidiophores. Two new species, viz ., Neohelicosporium suae and Parahelicomyces suae , one new record, Helicoma rufum , and three new collections, namely, H. rugosum, P. hyalosporus , and Tubeufia cylindrothecia are introduced based on morphological evidence and molecular phylogenetic analysis of combined ITS, LSU, tef 1- α, and RPB2 sequence data. Detailed descriptions and illustrations of these species are provided, and a morphological comparison with similar taxa is discussed.
The monotypic genus Excipulariopsis, with type species E. narsapurensis, was established based on the emended generic characteristics of Excipularia narsapurensis. Excipulariopsis has non-cupulated superficial conidiomata arising directly from stroma surrounded by thick walled dark brown subulate setae. The conidia are dark brown, broadly fusoid, having thick septa and truncate base. Phylogenetic analyses using ITS, LSU, TEF1α and RPB2 sequences positioned Excipulariopsis allied to Speiropsis in the family Wiesneriomycetaceae, as a sister family to Tubeufiaceae within Tubeufiales. This study resolved a long-standing ambiguity about the phylogenetic status of this unique sporodochial setose hyphomycetes from India.
