Figure - uploaded by Styliani A. Chasapi
Content may be subject to copyright.
NMR chemical shifts of organic and conjugated organic acids in the composition of SB berries methanolic extract.
Source publication
Sea buckthorn berries (Hippophaë rhamnoides L.) (SB) are considered as a fruit with a high nutritional value with a plethora of bioactive ingredients. The present work focusses on the analysis of the whole NMR metabolic profile of SB berries grown in an organic orchard of Meteora/Greece. In parallel, this study validates/highlights qualitative char...
Contexts in source publication
Context 1
... analysis reveals seven organic acids. Of them, three belong to the phenolic acids: vanillic, ferulic, and gallic acid in SB polar extract (Table 3). Phenolic acid and p-coumaric acid have been identified only as phenolic glycosides (Table 2). ...Context 2
... shifts in the 1 H NMR spectrum of SB methanolic extract indicate the feruloyl ester. The characteristic doublets (Table 3) [36]. The HMBC spectrum shows that this saccharide is further coupled with two other glucose units, giving long range correlations with the anomeric carbons at δ C 100.3 and 104.5. ...Context 3
... HMBC spectrum shows that this saccharide is further coupled with two other glucose units, giving long range correlations with the anomeric carbons at δ C 100.3 and 104.5. Other organic acids that dominate in fresh SB berries composition are malic, quinic and citric acid (Table 3). The total SB methanolic composition also bears a lipid part with the acyl chain's olefinic -CH=CH-1 H and 13 C signals assigned at δ H 5.36/δ C 131.0. ...Context 4
... identification of vitamin D was determined from the proton at H-14/C-14 position with δ H 2.20/δ C 52.9 [42]. Of great significance is vitamin C, also known as L-ascorbic acid, which is present in the methanolic extract of SB berries (Table 3) [43]. HSQC cross peaks at δ H 4.82/δ C 77.81, which corresponds to H-4/C-4 with length range correlations to C-5, C-6 and C-1 atoms at δ C 72.3, 66.2, 179.3 (Table 5). ...Citations
... Both molecules may exhibit a very similar spectral pattern due to the same saccharide backbone, although there might be a few indicative changes due to the position of the substitution usually at the resonances of the H-3 and H-5 signals (Yamada et al., 1992;Cimino et al., 2001). The most significant proton and carbon resonances of SAA's functional groups which have been already reported and characterized by various experts on the field are summarized in Table 2 (Bubb, 2003;Clé ment et al., 2010;Post et al., 2012;Tareq et al., 2014;Sarpal et al., 2016;Tan et al., 2016;Zompra et al., 2021). ...
Synthetic surfactants are used in several industries, including manufacturing, pharmaceutical and cosmetic’s, food and feed, agriculture, petroleum and environmental remediation for their ability to adsorb to fluid and solid-water interfaces. However, their widespread use and their synthetic preparation through environmentally unfavorable processes counterbalances the value of this class of reagents. This fact has stimulated new efforts to exploit natural sources of surfactants, such as new classes of bacterial systems or manipulation of existing biological systems, that may produce, through an environmentally friendly process, new biodegradable surfactants and emulsifiers of high commercial value. A downside of microbial production of biobased chemicals such as these types of chemicals, is that their fermentation often yields crude materials consisting of several bioproducts with complex physical and chemical properties. Extraction, identification, and efficient characterization of biosurfactants from a crude mixture of biomolecules requires carefully designed, and detailed analytical processes using state-of-the-art methods. The purpose of this review article is to present the current state-of-the-art and future outlook on the various multidisciplinary biophysical methods applied in the discovery, extraction identification, and in-depth characterization of microbially-produced surface‐active compounds.
The inflammatory disorders represent a serious health issue. Certain Cissus species possess anti-inflammatory effect. Cissus rhombifolia Vahl. leaves' anti-inflammatory activities and phytoconstituents are poorly characterized. In this study, 38 constituents were tentatively characterized in Cissus rhombifolia Vahl. leaves' aqueous methanolic extract (CRLE) using high-performance liquid chromatography combined with mass spectrometry (HPLC-MS) and Proton Nuclear Magnetic Resonance (1H NMR). Myricetin, β-amyrin, and alliospiroside A, were isolated from CRLE using column chromatography. The anti-inflammatory effect of CRLE and its isolated compounds were studied in lipopolysaccharide (LPS)-induced RAW 264.7 cells. 3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT assay) was used to assess how CRLE and its isolated compounds affected cell viability. Further, its effects on the production of intracellular NO, and inflammatory cytokines cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), and interleukin 6 (IL-6) were assessed by the Griess test, and cytokine enzyme-linked immunosorbent assays, respectively. CRLE and its isolated compounds, myricetin, β-amyrin, and alliospiroside A decreased the NO production. Western blotting was performed to assess the protein expression levels of the inflammatory cytokines inducible nitric oxide synthase (iNOS). Alliospiroside A downregulated IL-6, TNF-α, and COX-2 and inhibited the expression of iNOS. CRLE and its compounds represent effective alternative candidate to treat inflammatory diseases.
Introduction
Premature adrenarche (PA) for long time was considered a benign condition but later has been connected to various diseases in childhood and adulthood which remains controversial.
Objective
To investigate the effect of premature adrenarche on the metabolic phenotype, and correlate the clinical and biochemical data with the metabolic profile of children with PA.
Methods
Nuclear magnetic resonance (NMR)-based untargeted and targeted metabolomic approach in combination with multivariate and univariate statistical analysis applied to study the metabolic profiles of children with PA. Plasma, serum, and urine samples were collected from fifty-two children with Idiopathic PA and forty-eight age-matched controls from the division of Pediatric Endocrinology of the University Hospital of Patras were enrolled.
Results
Metabolomic results showed that plasma and serum glucose, myo-inositol, amino acids, a population of unsaturated lipids, and esterified cholesterol were higher and significantly different in PA children. In the metabolic profiles of children with PA and age-matched control group a gradual increase of glucose and myo-inositol levels was observed in serum and plasma, which was positively correlated their body mass index standard deviation score (BMI SDS) values respectively. Urine ¹H NMR metabolic fingerprint of PA children showed positive correlation and a clustering-dependent relationship with their BMI and bone age (BA) respectively.
Conclusion
This study provides evidence that PA driven metabolic changes begin during the childhood and PA may has an inductive role in a BMI–driven increase of specific metabolites. Finally, urine may be considered as the best biofluid for identification of the PA metabolism as it reflects more clearly the PA metabolic fingerprint.
For sustainable sea buckthorn (Hippophae rhamnoides) berry production, the task at hand is to find an application for the large amount of biomass waste arising at harvesting. Sea buckthorn (SBT) vegetation is currently poorly studied. The purpose of this research was to assess the composition
and potential of SBT twigs as a source of valuable biologically active substances. Water and 50% EtOH extracts of twigs of three Latvian SBT cultivars with a high berry yield and quality, popular for cultivation in many countries (H. rhamnoides ‘Maria Bruvele’, ‘Tatiana’, ‘Botanicheskaya Lubitelskaya’), were investigated for the first time. The phytochemical composition (UHPLC-ESI-MS/MS analysis) and biological activity of the obtained hydrophilic extracts were determined. The highest yield of
polyphenolic compounds and serotonin was observed for ‘Maria Bruvele’. Hydrophilic extracts were investigated for radical scavenging activity (DPPH˙ test), antibacterial/antifungal activity against five pathogenic bacteria/yeast, cytotoxicity, and the enzymatic activity of alpha-amylase (via in vitro
testing), which is extremely important for the treatment of people with underweight, wasting, and malabsorption. The results showed a high potential of sea buckthorn biomass as a source of valuable biologically active compounds for the creation of preparations for the food industry, nutraceuticals, and cosmetics.
