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NCBI conserved domain search results for the ebony protein. The location of the 256 gRNA relative to the mature protein sequence is indicated. 257

NCBI conserved domain search results for the ebony protein. The location of the 256 gRNA relative to the mature protein sequence is indicated. 257

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Figure 1. Characterization of GAL4 and UAS vectors. 167 (A-C) Tribolium...
Figure 2. Construction and validation of the CRISPR gene disruption...
Figure 5. NCBI conserved domain search results for the ebony protein....
Expanding the genetic toolkit of Tribolium castaneum
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  • Feb 2018
The red flour beetle, Tribolium castaneum , is an important model insect and agricultural pest.However, many standard genetic tools are lacking or underdeveloped in this system. Here, we present a set of new reagents to augment existing Tribolium genetic tools. We demonstrate a new GAL4 driver line that employs the promoter of a ribosomal protein g...
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... We speculate that this difference is due to the location of the 240 gRNA target site. It lies within the seventh exon of ebony, which falls after the sequences 241 encoding all but one predicted functional domain of the protein (Fig. 5). However, this gRNA 242 falls at the start of the last domain of the protein, and disruption of this domain likely explains the 243 hypomorphic phenotype observed. Tribolium. Future variations/deviations of both p130der and p119der will include an attB site for 275 . CC-BY-NC-ND 4.0 International license under a not certified by peer ...