Fig 2 - uploaded by Yury Rovensky
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Mouse embryo cells on the polyvinylchloride plate with 40/~m deep grooves (a) 30 min after seeding, cells in the grooves; (b) 60 min after seeding. The cells begin to migrate from the grooves and to spread themselves on the side surfaces of the grooves. Microphotograph, x 100.
Source publication
Behaviour of mouse and chick embryo fibroblast-like cells and of neoplastic mouse fibroblasts of L strain on surfaces with orderly distributed grooves of various depth (from 5 to 65 μm) was studied. A few hours after seeding, normal embryo cells migrated from the grooves of a certain depth (more than 15 μm) and spread over the surface between the g...
Contexts in source publication
Context 1
... plates with 40 #m deep grooves: At 30 min after seeding most cells were located in the grooves. These cells were not spread ( fig. 2a). At 1-3 h the cells gradually migrated from the grooves and spread themselves upon the side surfaces of the grooves ( fig. 2b). Migrating cells gradually oriented themselves parallel with the grooves. At 3-6 h most cells were already localized in the intervals between the grooves. Later (at 10-18 h) the cell density in the intervals ...
Context 2
... plates with 40 #m deep grooves: At 30 min after seeding most cells were located in the grooves. These cells were not spread ( fig. 2a). At 1-3 h the cells gradually migrated from the grooves and spread themselves upon the side surfaces of the grooves ( fig. 2b). Migrating cells gradually oriented themselves parallel with the grooves. At 3-6 h most cells were already localized in the intervals between the grooves. Later (at 10-18 h) the cell density in the intervals between grooves gradually increased, but the cell distribution was unchanged. The number of cells per unit area near the bottom ...
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Citations
... These surfaces can control cellular interactions within micro and sub-micrometre dimensions, mimicking the organisation of cells [1]. Numerous studies have explored the fundamental principles of cell-surface interactions and have detailed cellular responses to various substrate topographical patterns [19][20][21][22][23][24][25][26][27][28][29][30][31][32][33]. ...
Microstructured surfaces are renowned for their unique properties, such as waterproofing and low adhesion, making them highly applicable in the biomedical field. These surfaces play a crucial role in influencing cell response by mimicking the native microenvironment of biological tissues. In this study, we engineered a series of biomimetic micropatterned surfaces using polydimethylsiloxane (PDMS) to explore their effects on primary breast cancer cell lines, contrasting these effects with those observed on conventional flat surfaces. The surface topography was varied to direct cells’ attachment, growth, and morphology. Our findings elucidate that surface-free energy is not merely a background factor but plays a decisive role in cell dynamics, strongly correlating with the spreading behaviour of breast cancer cells. Notably, on micropillar surfaces with high surface-free energy, an increase in the population of cancer cells was observed. Conversely, surfaces characterised by lower surface-free energies noted a reduction in cell viability. Moreover, the structural parameters, such as the gaps and diameters of the pillars, were found to critically influence cellular dispersion and adherence, underscoring the importance of the microstructures’ topography in biomedical applications. These insights pave the way for designing advanced microstructured surfaces tailored to specific cellular responses, opening new avenues for targeted cancer therapies and tissue engineering.
... These surfaces can control cellular interactions within micro and sub-micrometre dimensions, mimicking the organisation of cells (Alves et al., 2010). Numerous studies have explored the fundamental principles of cell-surface interactions and have detailed cellular responses to various substrate topographical patterns (Nikkhah et al., 2009;Bettinger et al., 2006;Turner et al., 2000;Charest et al., 2007;Dusseiller et al., 2005;Liao et al., 2003;Mai et al., 2007;Rovensky et al., 1971;Von Recum and Van Kooten, 1996;Peter Clark, 1994;Curtis and Wilkinson, 1998; van Kooten et al., 1998;Walboomers et al., 1999;Milner and Siedlecki, 2007;Wilkinson et al., 2002). Exploring how cells respond to surfaces featuring micrometer-scale topographical variations has involved using diverse natural and synthetic substrates. ...
Microstructured surfaces are renowned for their unique properties, such as waterproofing and low adhesion, making them highly applicable in the biomedical field. These surfaces play a crucial role in influencing cell response by mimicking the native microenvironment of biological tissues. In this study, we engineered a series of biomimetic micropatterned surfaces using polydime-thylsiloxane (PDMS) to explore their effects on primary breast cancer cell lines, contrasting these effects with those observed on conventional flat surfaces. The surface topography was varied to direct the attachment, growth, and morphology of cells. Our findings elucidate that surface-free energy is not merely a background factor but plays a decisive role in cell dynamics, strongly correlating with the spreading behaviour of breast cancer cells. Particularly, on micropillar surfaces with high surface-free energy, an increase in the population of cancer cells was observed. Conversely, surfaces characterised by lower surface-free energies noted a reduction in cell viability. Moreover, the structural parameters such as the gaps and diameters of the pillars were found to critically influence cellular dispersion and adherence, underscoring the importance of the microstructures' topography in biomedical applications. These insights pave the way for designing advanced microstructured surfaces tailored to specific cellular responses, opening new avenues for targeted cancer therapies and tissue engineering.
... Some authors have demonstrated that macrophages, epithelial cells and osteoblasts have a high trophism towards rough surfaces [22][23][24]. An implant's surface roughness has the capacity to precisely select one population of cells and change their functions [22,25]. ...
Titanium is the most frequently employed material in implantology, because of its high degree of biocompatibility. The properties of materials are crucial for osteointegration; therefore, great effort from researchers has been devoted to improving the capabilities of titanium implant surfaces. In this context, graphene oxide represents a promising nanomaterial because of its exceptional physical and chemical qualities. Many authors in recent years have concentrated their research on the use of graphene in biomedical applications such as tissue engineering, antimicrobial materials, and implants. According to recent studies, graphene coatings may considerably increase osteogenic differentiation of bone marrow mesenchymal stem cells in vitro by the regulation of FAK/P38 signaling pathway, and can encourage the osteointegration of dental implants in vivo. However, further studies, especially on human subjects, are necessary to validate these potential applications. The aim of this work was to evaluate the effects of graphene on bone metabolism and the advantages of its use in implantology. A systematic review of literature was performed on PubMed, Web of Science and Scopus databases, and the articles investigating the role of graphene to functionalize dental implant surfaces and his interactions with the host tissue were analyzed.
... The line is one of the earliest [236,237] and most commonly used MP to control cell morphology, which has been used as parallel lines and in line alignments such as grids. In general, lines are used with different widths and can lead to different cell fates. ...
Human cells are both advanced pharmaceutical drugs and ‘drug deliverers’. However, functional control prior to or after cell implantation remains challenging. Micro-patterning cells through geometrically defined adhesion sites allows controlling morphogenesis, polarity, cellular mechanics, proliferation, migration, differentiation, stemness, cell–cell interactions, collective cell behavior, and likely immuno-modulatory properties. Consequently, generating micro-patterned therapeutic cells is a promising idea that has not yet been realized and few if any steps have been undertaken in this direction. This review highlights potential therapeutic applications, summarizes comprehensively the many cell functions that have been successfully controlled through micro-patterning, details the established micro-pattern designs, introduces the available fabrication technologies to the non-specialized reader, and suggests a quality evaluation score. Such a broad review is not yet available but would facilitate the manufacturing of therapeutically patterned cell populations using micro-patterned cell-instructive biomaterials for improved functional control as drug delivery systems in the context of cells as pharmaceutical products.
... The line is one of the earliest [236,237] and most commonly used MP to control cell morphology, which has been used as parallel lines and in line alignments such as grids. In general, lines are used with different widths and can lead to different cell fates. ...
Human cells are both advanced pharmaceutical drugs and ‘drug deliverers’. However, functional control prior to or after cell implantation remains challenging. Micro-patterning cells through geometrically defined adhesion sites allows controlling morphogenesis, polarity, cellular mechanics, proliferation, migration, differentiation, stemness, cell-cell interactions, collective cell behavior, and likely immuno-modulatory properties. Consequently, generating micro-patterned therapeutic cells is a promising idea that has not yet been realized and few if any steps have been undertaken in this direction. This review highlights potential therapeutic applications, summarizes comprehensively the many cell functions that have been successfully controlled through micro-patterning, details the established micro-pattern designs, introduces the available fabrication technologies to the non-specialized reader, and suggests a quality evaluation score. Such a broad review is not yet available but would facilitate the manufacturing of therapeutically patterned cell populations using micro-patterned cell-instructive biomaterials for improved functional control as drug delivery systems in the context of cells as pharmaceutical products.
... The surfaces provided for cell attachment can directly affect cell shape and cell function. Cells grown on grooved substrata are more round than cells grown on flat, smooth substrata [9]. A number of cellular properties, including growth [10], secretion of proteinases [9], and gene expression [11], are affected by cell shape. ...
... Cells grown on grooved substrata are more round than cells grown on flat, smooth substrata [9]. A number of cellular properties, including growth [10], secretion of proteinases [9], and gene expression [11], are affected by cell shape. The surface texture on an implant has the potential of specifically selecting a certain population of cells and altering their functions [12]. ...
Background
Scientific evidence in the field of implant dentistry of the past 20 years established that titanium rough surfaces have shown improved osseointegration rates. In a majority of dental implants, the surface microroughness was obtained by grit blasting and/or acid etching. The aim of the study was to evaluate in vivo two different highly hydrophilic surfaces at different experimental times.
Methods
Calcium-modified (CA) and SLActive surfaces were evaluated and a total of 18 implants for each type of surface were positioned into the rabbit articular femoral knee-joint in a split model experiment, and they were evaluated histologically and histomorphometrically at 15, 30, and 60 days of healing.
Results
Bone-implant contact (BIC) at the two-implant surfaces was significantly different in favor of the CA surface at 15 days (p = 0.027), while SLActive displayed not significantly higher values at 30 (p = 0.51) and 60 days (p = 0.061).
Conclusion
Both implant surfaces show an intimate interaction with newly formed bone.
... Micro-structured HA generally resulted in cell morphology change and consequently altered cell function changes [12][13][14][15] . Henry et al. 16 demonstrated that mouse pre-osteoblast cells (MC3T3-E1) were better arranged along micro-grooves with a groove width of 5 μm for the poly(ε-caprolactone) triacrylate/ HA substrates. ...
Hydroxyapatite (HA) is the principal inorganic component of bones and teeth and has been widely used as a bone repair material because of its good biocompatibility and bioactivity. Understanding the interactions between proteins and HA is crucial for designing biomaterials for bone regeneration. In this study, we evaluated the effects of atomic-level nano-structured HA (110) surfaces on the adsorption of bone morphogenetic protein-7 (BMP-7) and its derived peptide (KQLNALSVLYFDD) using molecular dynamics and density functional theory methods. The results indicated that the atomic-level morphology of HA significantly affected the interaction strength between proteins and HA substrates. The interactions of BMP-7 and its derived peptide with nano-concave and nano-pillar HA surfaces were stronger than those with flat or nano-groove HA surfaces. The results also revealed that if the groove size of nano-structured HA surfaces matched that of residues in the protein or peptide, these residues were likely to spread into the grooves of the nano-groove, nano-concave, and nano-pillar HA, further strengthening the interactions. These results are helpful in better understanding the adsorption behaviors of proteins onto nano-structured HA surfaces, and provide theoretical guidance for designing novel bioceramic materials for bone regeneration and tissue engineering.
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... He observed changes in cell behaviour with different substrates. 8 Work on topographical control of cells was resumed in 1970s by Maroudas 9 and Rovensky et al. 10 Since then extensive work has been done on textured surfaces for medical implants. The hypothesis is that microtexture on implants can benefit tissue regeneration because of their structural resemblance to natural ECM networks. ...
... Several researchers have shown that microgrooves on surfaces affect endothelial cell alignment, migration and proliferation rate. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] Our findings showed that cell adhesion and proliferation also improved on photoembossed textured surfaces compared to their smooth counterparts. Figure 9 shows a SEM image of a cell along the groove of a photoembossed substrate revealing focal adhesions. ...
We have shown previously that PMMA-acrylate photopolymers are biocomopatible and can exhibit improved cell adhesion compared to PMMA, due to an increase in negative surface charge caused by UV radiation PLGA has been used widely in soft tissue regeneration due to its high biocompatibility and cell adhesion. This polymer is also biodegradable and can be utilised in the field of vascular regeneration. In this study, PLGA is blended with a triacrylate monomer (TPETA) to create a degradable photopolymer blend. Surface relief structures are formed on this PLGA-TPETA by photoembossing. An optimum height of 950 nm was achieved for a 10 µm pitch with the height of these relief structures being controlled by changing UV intensity, processing temperature and time. Degradation studies of this blend revealed a bulk degradation mechanism with PLGA-TPETA degrading slower compared to pure PLGA. We also evaluated the adhesion of human umbilical vein endothelial cells (HUVECs) on both smooth and textured PLGA-TPETA films. Embossed PLGA-TPETA films showed improved cell adhesion compared to smooth substrates. Furthermore, HUVECs proliferated faster on the embossed surface compared to their smooth counterparts. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2016.
... Cell can 'sense' the surface topography and then take reaction to these surface cues. The interaction between substrates and cells is achieved through the effort of the cytoskeleton, the extracellular matrix (ECM) [3][4][5] and the focal adhesions [6]. ...
