Morphology of Neosartorya spinosa KKU-1NK1. (A), Colonies on Czapek's agar (CZA) 14-day-old at 30°C; (B), Reverse plate of colonies on CZA; (C), Fungal growth in Potato dextrose broth 35 days at 30°C; (D), Asci and ascospores; (E), SEM of ascospores; (F), Ascomata (cleistothecium-formed); (G-H), Conidial head and vesicle.  

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... 14 days of incubation at 30°C, colonies morphology of the fungus on Czapek's solution agar showed often growing rapidly with margin entire or irregular, presenting a dissected appearance, characterized by abundant cleistothecia in colors near cartridge buff to light buff ( Figure 1A) [21]. The reverse plate of Czapek's solution agar showed brown shade ( Figure 1B) while this fungal specie secreted the violet exudate in potato dextrose broth after an incubation time of 21 days ( Figure 1C). ...

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... 14 days of incubation at 30°C, colonies morphology of the fungus on Czapek's solution agar showed often growing rapidly with margin entire or irregular, presenting a dissected appearance, characterized by abundant cleistothecia in colors near cartridge buff to light buff ( Figure 1A) [21]. The reverse plate of Czapek's solution agar showed brown shade ( Figure 1B) while this fungal specie secreted the violet exudate in potato dextrose broth after an incubation time of 21 days ( Figure 1C). Cleistothecia globose ( Figure 1F), mostly 200 to 300 μm; asci eight-spored; ascospore with nearly globose spore bodies ( Figure 1D) averaging 3.0 to 5.0 μm in diameter with two widely separated (1.0 to 1.5 μm) equatorial crests ranging from 1.0 to 2.0 mm wide and with convex surfaces bearing spine like projections ranging from <0.5 μm to 2.0 μm, were observed. ...

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... 14 days of incubation at 30°C, colonies morphology of the fungus on Czapek's solution agar showed often growing rapidly with margin entire or irregular, presenting a dissected appearance, characterized by abundant cleistothecia in colors near cartridge buff to light buff ( Figure 1A) [21]. The reverse plate of Czapek's solution agar showed brown shade ( Figure 1B) while this fungal specie secreted the violet exudate in potato dextrose broth after an incubation time of 21 days ( Figure 1C). Cleistothecia globose ( Figure 1F), mostly 200 to 300 μm; asci eight-spored; ascospore with nearly globose spore bodies ( Figure 1D) averaging 3.0 to 5.0 μm in diameter with two widely separated (1.0 to 1.5 μm) equatorial crests ranging from 1.0 to 2.0 mm wide and with convex surfaces bearing spine like projections ranging from <0.5 μm to 2.0 μm, were observed. ...

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... reverse plate of Czapek's solution agar showed brown shade ( Figure 1B) while this fungal specie secreted the violet exudate in potato dextrose broth after an incubation time of 21 days ( Figure 1C). Cleistothecia globose ( Figure 1F), mostly 200 to 300 μm; asci eight-spored; ascospore with nearly globose spore bodies ( Figure 1D) averaging 3.0 to 5.0 μm in diameter with two widely separated (1.0 to 1.5 μm) equatorial crests ranging from 1.0 to 2.0 mm wide and with convex surfaces bearing spine like projections ranging from <0.5 μm to 2.0 μm, were observed. Conidial heads showed characteristic flask-shaped vesicle with uniseriate sterigmata ( Figure 1G-H) [24]. ...

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... reverse plate of Czapek's solution agar showed brown shade ( Figure 1B) while this fungal specie secreted the violet exudate in potato dextrose broth after an incubation time of 21 days ( Figure 1C). Cleistothecia globose ( Figure 1F), mostly 200 to 300 μm; asci eight-spored; ascospore with nearly globose spore bodies ( Figure 1D) averaging 3.0 to 5.0 μm in diameter with two widely separated (1.0 to 1.5 μm) equatorial crests ranging from 1.0 to 2.0 mm wide and with convex surfaces bearing spine like projections ranging from <0.5 μm to 2.0 μm, were observed. Conidial heads showed characteristic flask-shaped vesicle with uniseriate sterigmata ( Figure 1G-H) [24]. ...

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... globose ( Figure 1F), mostly 200 to 300 μm; asci eight-spored; ascospore with nearly globose spore bodies ( Figure 1D) averaging 3.0 to 5.0 μm in diameter with two widely separated (1.0 to 1.5 μm) equatorial crests ranging from 1.0 to 2.0 mm wide and with convex surfaces bearing spine like projections ranging from <0.5 μm to 2.0 μm, were observed. Conidial heads showed characteristic flask-shaped vesicle with uniseriate sterigmata ( Figure 1G-H) [24]. SEM observations showed that the ascospores of KKU-1NK1 have convex surfaces with distinct spines ( Figure 1E). ...

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... heads showed characteristic flask-shaped vesicle with uniseriate sterigmata ( Figure 1G-H) [24]. SEM observations showed that the ascospores of KKU-1NK1 have convex surfaces with distinct spines ( Figure 1E). The fungal isolate KKU-1NK1 were initially identified using morphological characteristics as Neosartor ya spinosa. ...