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Morphological variations associated with heat shock recovery process in high temperature tolerant strains of Trichoderma
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The study focuses on the morphological and physiological cell responses to high temperature stress in thermotolerant isolates of T. asperellum characteristic comparison to susceptible micros reduced intracellular spaces and mitochondrial count. The observations have supported the hypothesis of reduced metabolism during stress conditions. TEM analys...
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... the biochemical analysis revealed that both these isolates accumulate a higher concentration of trehalose, a known compatible solute, during heat shock (Poosapati et al., 2014). The morphological differences of thermotolerant isolates and other Trichoderma isolates of IIOR repository were observed after recovery from heat stress and are tabulated in Table 2. ...Citations
... C C C C C -+++ +++ +++ ++ --Yg+ Dg Yg+ Dg Yg+ Dg Dg --Trichoderma isolates have been shown to induce plant resistance under abiotic stresses, among the 13 isolates tested, T3, T6, T7, T10, T12 and T13 exhibited higher abiotic stress tolerance to either drought, salinity or high temperature. Nevertheless, Trichoderma by themselves are not immune to abiotic stress like moisture deficiency, higher temperature, etc., that tend to cause morphological, physiological, biochemical and molecular changes and adversely affect the beneficial consequences of these bioagents (Sowmya et al., 2016). Even though Trichoderma promote plant growth and induce resistance to biotic and abiotic stresses (Hermosa et al., 2013), their role as bio pesticide has primarily contributed to their commercial success as bio-agents. ...
Climate change and climatic variability are major over-riding concerns for Indian agriculture impacting ultimate farm profitability and sustainability.Both biotic and abiotic stresses are influenced by these phenomena. In the current study 13Trichoderma isolates were screened in-vitroat CRIDA - Hyderabad during 2018-2019, for their biotic and abiotic stress tolerance levels.In-vitro antagonistic activity with phytopathogens Macrophominaphaseolina (Mp), Fusariumoxysporumf.sp. ricini (FoR), Rhizoctoniasolani (Rs) and Sclerotiumrolfsii (Sr) revealed that T6,T7,T9,T12 and T13 isolates were most effective against Mp, FoR and Rs with 97-100% inhibition, in Rs T4, T12 and T13 with 69-75% inhibition were found to be best.In plant growth promotion traits, T5 was found to be the highest producer of IAA with 37.9g/mL, T6 and T8 produced the highest amounts of HCN (39.2ppm). Similarly ammonia was produced highest in T5 and T13 while in siderophore production was highest in T10 isolate with 80 SU. Under drought conditions, T3, T12, T11 and T13 isolates were tolerant up to -1.8MPa and in salinity conditionsT6, T12 and T13 isolates were resistant up to 220 dSm-1. T6, T12 and T13 isolates were found to sustain elevated temperatures up to 43°C. Most of the Trichoderma isolates were found to be effective antagonists and abiotic stress tolerant.
... C C C C C -+++ +++ +++ ++ --Yg+ Dg Yg+ Dg Yg+ Dg Dg --Trichoderma isolates have been shown to induce plant resistance under abiotic stresses, among the 13 isolates tested, T3, T6, T7, T10, T12 and T13 exhibited higher abiotic stress tolerance to either drought, salinity or high temperature. Nevertheless, Trichoderma by themselves are not immune to abiotic stress like moisture deficiency, higher temperature, etc., that tend to cause morphological, physiological, biochemical and molecular changes and adversely affect the beneficial consequences of these bioagents (Sowmya et al., 2016). Even though Trichoderma promote plant growth and induce resistance to biotic and abiotic stresses (Hermosa et al., 2013), their role as bio pesticide has primarily contributed to their commercial success as bio-agents. ...
Climate change and climatic variability are major over-riding concerns for Indian agriculture impacting ultimate farm profitability and sustainability.Both biotic and abiotic stresses are influenced by these phenomena. In the current study 13Trichoderma isolates were screened in-vitroat CRIDA - Hyderabad during 2018-2019, for their biotic and abiotic stress tolerance levels.In-vitro antagonistic activity with phytopathogens Macrophominaphaseolina (Mp), Fusariumoxysporumf.sp. ricini (FoR), Rhizoctoniasolani (Rs) and Sclerotiumrolfsii (Sr) revealed that T6,T7,T9,T12 and T13 isolates were most effective against Mp, FoR and Rs with 97-100% inhibition, in Rs T4, T12 and T13 with 69-75% inhibition were found to be best.In plant growth promotion traits, T5 was found to be the highest producer of IAA with 37.9g/mL, T6 and T8 produced the highest amounts of HCN (39.2ppm). Similarly ammonia was produced highest in T5 and T13 while in siderophore production was highest in T10 isolate with 80 SU. Under drought conditions, T3, T12, T11 and T13 isolates were tolerant up to -1.8MPa and in salinity conditionsT6, T12 and T13 isolates were resistant up to 220 dSm-1 . T6, T12 and T13 isolates were found to sustain elevated temperatures up to 43°C. Most of the Trichoderma isolates were found to be effective antagonists and abiotic stress tolerant.
... In spite of the growing genomic resources in Trichoderma species (the genomes of several Trichoderma species have been sequenced [https://jgi.doe.gov/search-results/?q= trichoderma, accessed on 31 October 2021] and ESTs from several species of Tricho derma being available in the TrichoEST database [43,44], much of the research so far has focused on studies involving plant-pathogen-bioagent interactions [45], mycoparasitim [46], biocontrol related genes and enzymes [47,48], and proteases produced by bio-agents [3,[49][50][51]. But there are only a few reports on the molecular changes associated with heat stress conditions in Trichoderma [52]. ...
Several species of the soil borne fungus of the genus Trichoderma are known to be versatile, opportunistic plant symbionts and are the most successful biocontrol agents used in today's agriculture. To be successful in field conditions, the fungus must endure varying climatic conditions. Studies have indicated that a high atmospheric temperature coupled with low humidity is a major factor in the inconsistent performance of Trichoderma under field conditions. Understanding the molecular modulations associated with Trichoderma that persist and deliver under abiotic stress conditions will aid in exploiting the value of these organisms for such uses. In this study, a comparative proteomic analysis, using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/time-of-flight (MALDI-TOF-TOF) mass spectrometry, was used to identify proteins associated with thermotolerance in two thermotolerant isolates of Trichoderma: T. longibrachiatum 673, TaDOR673 and T. asperellum 7316, TaDOR7316; with 32 differentially expressed proteins being identified. Sequence homology and conserved domains were used to identify these proteins and to assign a probable function to them. The thermotolerant isolate, TaDOR673, seemed to employ the stress signaling MAPK pathways and heat shock response pathways to combat the stress condition, whereas the moderately tolerant isolate, TaDOR7316, seemed to adapt to high-temperature conditions by reducing the accumulation of misfolded proteins through an unfolded protein response pathway and autophagy. In addition, there were unique, as well as common, proteins that were differentially expressed in the two isolates studied.
The aim of this study was to determine the phylogenetic relationships of Trichoderma isolates obtained from NLS Tobacco region. Utilizing the sequence analysis of internal transcribed spacer-1 (ITS-1) region of the ribosomal DNA the rDNA of Five Trichoderma isolates was amplified by polymerase chain reaction (PCR) using universal primers (ITS-1 and ITS-4). PCR products were purified and these purified products were used to amplify the ITS-4 region of the five Trichoderma isolates. The amplified DNA was sequenced and aligned against ex-type strain sequences from TrichoBLAST and established Trichoderma taxonomy. Two isolates were positively identified as Trichoderma harzianum and three isolates were identified as Trichoderma aesperulum which were used as an outgroup in these analyses. The ITS-1 region sequences have been used as the reference’s sequence and this could be further used for future study involving the identification and taxonomy of Trichoderma Amplification of ITS-1 region of the rDNA. This method proved as a rapid and reliable technique for identifying Trichoderma strains successfully.
