Table 1 - uploaded by Dr.KANNAN MARIKANI
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Objective: To screen the high yielding PHB producing bacterial strains and its characterization by phenotypic methods and PHB production was optimized by using different carbon sources, different levels of pH and temperature. Methods: The oil contaminated soils of Virudhunagar town were chosen for the isolation of PHB producing bacterial species. T...
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... incubation for 3-4 days at 37°C, the colonies found in nutrient agar plates were individually isolated and subjected for the screening of PHB production using Sudan Black B. In microscopic observation the lipid inclusion granules stained blue-black in colour was pure cultured. Seven different microbial isolates showed PHB granules in the cell and its morphological observations were tabulated (Table 1). ...
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Microorganisms produce copious macromolecules, many of which harbor tremendous biotechnological potential. One such macromolecule is polyhydroxyalkanoates (PHA). It is a key substance formed as inclusion bodies by bacteria to accumulate, and reserve growth materials while confronting various stress conditions. PHA is chosen as an alternative for th...
Citations
... Quanti cation of PHB by Crotonic acid assay: 2.6.1. PHB stock preparation:The stock was prepared by dissolving 10 mg of standard bacteriological PHB in 10 ml of sulphuric acid[43].Varied concentration of PHB weighing from 100 µg to 1000 µg/ml was taken in a series of test tubes. It was made up to 1ml with distilled water. ...
... Sulphuric acid was added carefully through the sides of the test tube. The tubes were incubated in a boiling water bath for about 40 minutes[43]. ...
Plastic pollution need to be resolved as it affects air, water, land. The favourable alternative for plastics would be Polyhydroxybutyrate (PHB) from bacterial origin, which are biodegradable and biocompatible biopolymers. Focus on the PHB producing bacteria is done by collecting garden soil sample. Five colonies of Sudan black blue positive isolates were chosen, extracted, and produced. One of the strains (SM1) - a potent producer as confirmed by crotonic acid assay, was further subjected to large scale production. The PHB thus produced was analysed using Fourier Transform Infra-Red spectroscopy (FTIR) and Gas Chromatography Mass Spectrometry (GCMS) to confirm the presence of functional groups. X-Ray crystallography revealed that it is of crystalline nature and are pictured by Scanning electron microscopy photography. DNA was isolated from the strain SM1, and the gene for 16S rRNA has been sequenced and submitted in GENBANK, (Accession No: MZ363886). The organism was found to be Bacillus cereus as predicted by 16S rRNA and NCBI BLAST. A phylogenetic tree was constructed using MEGA software. Bioplastic preparation was done under laboratory scale and the produced bioplastic was successfully degraded using Pseudomonas species . The prepared bioplastic from bacteria was biodegradable and eco-friendly.
