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Prebiotics such as mannan-oligosaccharides (MOS) are a promising approach to improve performance and disease resistance in shrimp. To improve prebiotic utilization, we investigated the potential probiotics and their feasibility of synbiotic use in vitro. Two bacterial isolates, Man26 and Man122, were isolated from shrimp intestines and screened for...
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... previously determined complete full genome sequences of our bacterial candidates, Man26 and Man122, in which they showed similarity to Niallia sp. and Bacillus sp., respectively [43]. The morphological characteristics of the two isolates showed that both Man26 and Man122 were Gram positive with a rod shape ( Table 1). The colony of Man26 had a white and shiny colony while Man122 were white with a dull texture. ...Similar publications
The effects of dietary mannan oligosaccharide (MOS) supplementation in a biofloc system on growth performance, whole‐body proximate composition, whole‐body fatty acids, nonspecific immune parameters, antioxidant enzyme activities, digestive enzyme activity, and gut microbiota of brown shrimp, Farfantepenaeus aztecus, were investigated. Shrimp were...
Citations
... Source of Enzyme The production of mannooligosaccharides (MOS) through the enzymatic treatment of agricultural waste, such as copra meal, highlights a significant advancement in the utilization of renewable resources for generating valuable prebiotic compounds. In the study by Sathitkowitchai et al., the team utilized a genetically engineered endo-β-(1,4)-mannanase, derived from B. subtilis but expressed in Escherichia coli, demonstrating the enzyme's capacity to effectively target and break down mannans into MOS with degrees of polymerization ranging from 4 to 7 [147]. This process emphasizes the enzyme's high stability and activity under specific conditions, showcasing its potential for scalable production. ...
Lignocellulosic wastes, primarily from agricultural by-products, are a renewable resource increasingly used in the sustainable production of oligosaccharides, significantly contributing to the growing bioeconomy. This innovative utilization of biological resources aligns with the global shift towards sustainable development, focusing on creating products such as food, feed, and bioenergy from renewable sources. Oligosaccharides, specialized carbohydrates, are synthesized either chemically or more eco-friendly, biologically. Biological synthesis often involves enzymes or whole-cell systems to transform lignocellulosic wastes into these valuable sugars. As functional food supplements, oligosaccharides play a crucial role in human and animal health. They serve as prebiotics, indigestible components that promote the proliferation of beneficial gut microbiota, especially within the colon. This positive impact on gut flora is essential for boosting the immune system and regulating physiological functions. Important prebiotics, including galactooligosaccharides (GOS), xylooligosaccharides (XOS), fructooligosaccharides (FOS), mannan-oligosaccharides (MOS), and isomaltooligosaccharides (IMOS), are produced through methods involving enzymes or the use of whole cells, with agricultural waste as substrates. Recent advancements focus on refining these biological processes for oligosaccharide synthesis using lignocellulosic substrates, emphasizing the principles of a circular bioeconomy, which promotes resource reuse and recycling. This review highlights the potential and challenges in the biological synthesis of oligosaccharides from renewable resources. It underscores the need for innovation in process optimization and commercialization strategies to fully exploit lignocellulosic wastes. This approach not only contributes to sustainable product development, but also opens new avenues for the profitable and environmentally friendly utilization of agricultural residues, marking a significant step forward in the bio-based industry.
... Lo resultados aquí obtenidos aumentan las posibilidades de utilizar a Bacillus subtilis E-44; esta vez en la producción de enzimas que mejoren la calidad de los alimentos ricos en fibra, lo cual incrementa el efecto probiótico de la misma (Luise et al., 2022). Las celulasas, mananasas y xilanasas son hidrolasas que comúnmente se emplean en la producción animal (Sathitkowitchai et al., 2022). ...
los) autor (es), Revista de Producción Animal 2020. Este artículo se distribuye bajo los términos de la licencia internacional Attribution-NonCommercial 4.0 (https://creativecommons.org/licenses/by-nc/4.0/), asumida por las colecciones de revistas científicas de acceso abierto, según lo recomendado por la Declaración de Budapest, la que puede consultarse en: Budapest
... Lo resultados aquí obtenidos aumentan las posibilidades de utilizar a Bacillus subtilis E-44; esta vez en la producción de enzimas que mejoren la calidad de los alimentos ricos en fibra, lo cual incrementa el efecto probiótico de la misma (Luise et al., 2022). Las celulasas, mananasas y xilanasas son hidrolasas que comúnmente se emplean en la producción animal (Sathitkowitchai et al., 2022). ...
Antecedentes:
El incremento de la conciencia sobre los problemas medioambientales ha conducido a la implementación de alternativas ecológicas en la producción industrial. Las celulasas y las hemicelulasas son las enzimas más empleadas a nivel industrial; su uso garantiza la eficacia de los procesos productivos con la disminución en el empleo de sustancias químicas que contaminan el medio ambiente.
Objetivo:
Seleccionar cepas de Bacillus spp. productoras de celulasas, β-mananasas y xilanasas para su utilización en la alimentación animal.
Materiales y métodos:
Se estudiaron diez cepas las cuales se inocularon en placas con medio mínimo suplementado con xilano de haya, goma de algarrobo y carboximetilcelulosa para la producción de xilanasas, β-mananasas y endocelulasas respectivamente. Las placas se incubaron a 37 ºC durante 24 horas y se cubrieron con una disolución de rojo congo al 0,5%. Se determinó el diámetro de la zona de hidrólisis y el de la colonia para calcular el índice de potencia.
Resultados:
De las cepas evaluadas todas mostraron la capacidad de producir las enzimas en estudio. El 23,3% de las actividades enzimáticas estudiadas se consideró como buena. Bacillus subtilis E-44 resultó como la cepa que manifestó la mejor actividad enzimática en los tres sustratos evaluados con índices de potencia de 3,01 ± 1,18; 3,82 ± 0,31 y 4,22 ± 0,23 para celulasas, β-mananasas y xilanasas respectivamente.
Conclusiones:
Bacillus subtilis E-44 se seleccionó como la mejor cepa en la producción de celulasas y hemicelulasas, lo que incrementa las potencialidades de su uso en la alimentación animal y en la industria de los alimentos.
... Under these circumstances, the identification of interventions able to stimulate the activity of commensal probiotic-like bacteria seems the most plausible solution to control the activity of bacterial pathogens such as V. parahaemolyticus [5,6]. The approach of using dietary supplements to stimulate the commensal bacteria, to improve performance and disease resistance in shrimp, was recently described when two commensal mannanase-secreting bacteria (Man26 and Man122) were able to increase mannan digestion and potentially improve absorption and utilization of feedstuffs [7]. Stimulating the growth of probiotic bacteria (e.g., F. prausnitzii) and increase butyrate production was previously achieved-achieved with oligosaccharides and resulted in improved gut health and increased resistance to infections [8]. ...
Increasing the abundance of probiotic bacteria in the gut requires either direct dietary supplementation or the inclusion of feed additives able to support the growth of beneficial commensal bacteria. In crustaceans, the increased presence of probiotic-like bacteria in the gut, including of Faecalibacterium prausnitzii (F. prausnitzii), will guarantee a positive health status and a gut environment that will ensure enhanced performance. The aim of this study was to investigate if a mixture of organic acids, AuraAqua (Aq) can stimulate the growth and the anti-pathogenic efficacy of F. prausnitzii through a combination of in vitro and ex vivo models. The results showed that 0.5% Aq was able to improve the growth rate of F. prausnitzii in vitro and in an ex vivo shrimp gut model. Moreover, we were able to demonstrate that Aq increases butyrate production and cellulose degradation in culture or in the shrimp gut model. The growth-stimulating effect of Aq also led to an improved and anti-pathogenic effect against Vibrio parahaemolyticus in a co-culture experiment with shrimp gut primary epithelial cells (SGP). In conclusion, our work demonstrates that Aq can stimulate the growth of F. prausnitzii, increase the production of short-chain fatty acid (SCFA) butyrate, improve substrate digestion, and prevent V. parahaemolyticus invasion of SGP cells.
