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p class="Els-Abstract-text">The aim of this research is to obtain indigenous fungus producing lignin peroxidase from the Cocoa Plantation in Sepawon Kediri Regency, Indonesia. Isolation was done using Minimum Salt Medium alkali lignin (MSM - L), purification was performed using monospore technique, lignin peroxidase activity was screened by agar me...
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... the conidiophores, the shape of conidia which have been known for a globose or ellipsodial and yellow [9]. Sporotrichum generally present in wet material plant and rotting or decayed wood [11]. It supports the identification, since the sample source of KLUM 1 was cocoa bark weathered. Based on morphological characteristics analysis of KLUM 2 (Fig. 5) showed that it has septate hyphae, the shape of conidiophores elongated, having clamp connection, conidia aleuriosporus, globose or ellipsoidal, thick-walled, readily detached. The study of literature that has been done [9,10] showed that KLUM 2 resemblance to the genus Crysosporium sp (Fig. 3). Similarities KLUM 2 with Crysosporium sp ...
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Our study is interested in isolating fungi from the wood parts of cedar trunks withered and identifying these lignivorous fungi. A sample was isolated from the cedar grove of Tazekka National Park located south of the city of Taza in Morocco. The culture and purification of the isolated fungus were done on a water agar medium and then on a PDA medi...
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... For lignin peroxidase activity, the method was modified from Delila et al. 33 : In triplicate, the fungal plug was transferred to a PDA plate, supplemented with methylene blue (0.0065%, Sigma-Aldrich), and incubated at room temperature, in the dark, for 14 days. A clear zone around the colony, caused by the disappearance of the blue colour of the dye, confirmed the presence of peroxidase or decolorization of methylene blue dyes. ...
Cookeina sulcipes and C. tricholoma are a cup fungi (Ascomycota) collected in Saraburi, Thailand. The fungi have been isolated, cultured and confirmed as respective species. For morphology, both Cookeina sp. are white mycelium and the growth rate on potato dextrose agar (PDA) result present C. sulcipes is faster than C. tricholoma. The molecular characterization from a rapid and simple DNA extraction method that is modified based on thermolysis method, The DNA extraction is finish in thirty minutes and efficiency to continuous with polymerase chain reaction (PCR) amplification to fungi species level identification. The DNA sequence from internal transcribed spacer (ITS) gene regions by universal primer pairs ITS5/ITS4 is effective to confirm Cookeina species level that C. sulcipes has 616 bp and C. tricholoma has 570 bp. Including, DNA sequence of large subunit (LSU) gene regions by universal primer pairs LROR/LR5 is generate that C. sulcipes has 912 bp and C. tricholoma has 906 bp. The cultures are screened for antibacterial activity by agar plug diffusion method and found that both isolates have been no activity against test strains (Bacillus subtilis, Escherichia coli, Kocuria rhizophila (Micrococcus luteus), Pseudomonas aeruginosa, Staphylococcus aureus and S. epidermidis). In a preliminary screening test of enzymes involved in plant biomass breakdown by agar plate method, both Cookeina sp. show cellulolytic and hemicellulolytic enzymatic activity, and manganese peroxidase (MnP) productivity. In contrast, only C. sulcipes had additional laccase activity. Neither isolate generate pectinolytic and lignin peroxidase (LiP) activities. Thus, Cookeina spp. proved the potentiality to break down lignocelluloses.
... Morfologi yang sama telah diperoleh pada penelitian Delila (2017) sekresi enzim ekstraseluler oleh kapang turut berperan serta dalam meningkatkan kandungan protein dalam suatu biomassa (Nelson & Suparjo, 2011). ...
Axonopus compressus as garden waste has the potential to become forage for livestock if it is processed by reducing its lignin content. Lignin is an anti-nutritional substance in animal feed whose presence can reduce the digestibility of feed nutrients. Mycelioptora thermophilia KLUM1 produces ligninase which can degrade lignin. The purpose of this study were to determine the effect of fermentation time and the number of spores of the Mycelioptora thermophilia KLUM1 on the levels of lignin, crude fat, and crude protein of Axonopus compressus. This research is a laboratory experimental study using a Completely Randomized Design (CRD) method, the first factor is fermentation time (14, and 21 days) and the second factor is the number of spores (1,72 x 107 , 4,29 x 107 , and 8,59 x 107 cells). The results showed that the best conditions for the levels of lignin and crude fat in this study were 8.59 x 107 cells for 21 days with a lignin content of 9.98% ± 0.23 and crude fat content of 3.43% ± 0 .04, while the best condition for crude protein content was 1.72 x 107 cells for 14 days of 11.64% ± 0.18.
