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Morphological and histological aspects of cryopreserved Vriesea reitzii seeds Leme & A.F. Costa (+LN) and non-cryopreserved (-LN) subjected to double staining of toluidine blue (TBO) and Lugol. a. seed overview, the embryo (arrowhead), plumose appendages (arrow). b. TBO: seed cross section, the cross section of the seed containing the embryo, endosperm structure, aleurone layer (arrowhead) and procambium or nuclei of embryonic stem cells (arrow). c-h. it can observe cell wall of thickened aleurone layer (arrowhead) and compounds starch grains (arrow): c. TBO and Lugol: 120 DAA +LN seed; d. TBO and Lugol: 135 DAA +LN seed; e. TBO and Lugol: 150 DAA +LN seed; f. TBO and Lugol: 120 DAA -LN seeds; g. TBO and Lugol: 135 DAA -LN seeds; h. TBO and Lugol: 150 DAA -LN seeds. Bar: 50 µm. DAA: Days after anthesis. TBO: Toluidine Blue. LN: Liquid nitrogen.
Source publication
Vriesea reitzii is an endemic bromeliad from the Atlantic Rainforest. The objective of this research was to evaluate the cryopreservation using the method of direct immersion of its seeds, collected from capsules at 120, 135, and 150 days after anthesis (DAA). The water content was determined before cryopreservation, while the germination percentag...
