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Midsagittal reference line for histomorphometric analysis (left: harvested calvaria, and right: soft X-ray image).
Source publication
The purpose of this study was to evaluate the capability of silk fibroin (SF) and tricalcium phosphate-coated silk fibroin (SF-TCP) as a bone defect replacement matrix when grafted in calvarial bone defect of rats in vivo. Total 114 calvarial critical size defects (5.0 mm in diameter) made on 57 adult female Sprague-Dawley rats were used for this s...
Context in source publication
Context 1
... histological analysis, fixed samples were decalcified with 10% formic acid. After bisecting the samples tangential to the sagittal suture, they were paraffin-embedded with the cut surface facing outward, sectioned in 4 µm thicknesses, and stained with hematoxylin-eosin (H&E) and Masson's Trichrome (MT). Sections from each block nearest the center were selected for histological and histomorphometric evaluation (Fig ...
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Objective
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Material and method
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