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Microscopic morphology of Fusarium matuoi on synthetic lownutrient agar (SNA). A Conidia showing continuous variation from aseptate, strongly curved C-shaped to multiseptate, falcate (D12507).
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A group of Fusarium isolates from slime flux similar to F. aquaeductuum produced unique, strongly curved, aseptate, C-shaped conidia. They were found to be identical to F. splendens nom. nud. Dried specimens from which F. splendens was originally isolated were reexamined and characterized as a new species of Cosmospora. Cosmospora matuoi sp. nov. i...
Citations
... S30023. The isolates CGMCC 3.24907, 3.24908, 3.24909 and 3.24910 grouped with other members of [29]. Phylogenetically, they are remotely related (Figure 1). ...
... Phylogenetically, they are remotely related (Figure 1). [9], while F. matuoi forms longer macroconidia (17-56 μm long) [29]. Phylogenetically, they are remotely related (Figure 1). ...
To explore the species diversity of the genus Fusicolla, specimens from Henan, Hubei and Jiangsu Provinces in China are examined, and three undescribed taxa are encountered. The morphological characteristics and DNA sequence analyses of the combined acl1, ITS, LSU, rpb2 and tub2 regions support their placement in Fusicolla and their recognition as new species. Fusicolla aeria sp. nov. is distinguished by the formation of abundant aerial mycelia on PDA, falcate, (1–)3-septate macroconidia 16–35 × 1.5–2.8 μm and subcylindrical, aseptate microconidia 7.5–13 × 0.8–1.1 μm. Fusicolla coralloidea sp. nov. has a coralloid colony on PDA, falcate, 2–5-septate macroconidia 38–70 × 2–4.5 μm and rod-shaped to ellipisoidal, aseptate microconidia 2–7 × 1–1.9 μm. Fusicolla filiformis sp. nov. is characterized by filiform, 2–6-septate macroconidia 28–58 × 1.5–2.3 μm and lacking microconidia. Morphological differences between these novel species and their close relatives are compared in detail. The previously recorded species of the genus in China are listed and a key to these taxa is provided.
... Strains were isolated from soil and rotten twig collected from Sichuan Province and Guangxi Autonomous Region in October 2020 and December 2019.The living ex-type strains were preserved in the China General Microbiological Culture Collection Center (CGMCC). Strains characteristics were described following Hosoya et al. (2004) and Lechat & Rossman (2017). To determine colony features and growth rates, the strains were grown on potato dextrose agar (PDA), malt extract agar (MEA), synthetic nutrient-poor agar (SNA), and oatmeal agar (OA) in 90 mm plastic Petri dishes at 25 °C with alternating periods of light and darkness (12 h/12 h). ...
... Fusicolla gigas is distinguished from F. matuoi by much larger macroconidia (32−80 × 2.3-3.8 µm vs. 7-56 × 1-3 μm) with more septa (3-9-septate vs. 0-6-septate) (Hosoya et al. 2004). Additionally, there are 28 bp and 17 bp divergences in the ITS and LSU regions between CGMCC 3.20680 and CBS 581.78. ...
... Fusicolla species show a broad range of habitat, such as decayed wood, stromata of other fungi, soil, flowing water, slime flux of trees, sewage, bone on wild boar and even air (Gräfenhan et al. 2011, Lechat & Rossman 2017, Jones et al. 2019, Singh et al. 2020 Lechat & Rossman, and F. siamensis were described with both of sexual and asexual stages (Grafenhan et al. 2011, Crous et al. 2016, 2021, Lechat & Rossman 2017. For a long time, F. matuoi was proposed for the asexual stage of Cosmospora matuoi Hosoya & Tubaki (Hosoya & Tubaki 2004, Gräfenhan et al. 2011. Lechat & Rossman (2017) et al. 2011, Crous et al. 2018, 2021, Jones et al. 2019, Forin et al. 2020, Singh et al. 2020. ...
Two new Fusicolla species are described and illustrated based on strains isolated from soil and rotten twig. Morphological features and DNA sequence analyses of combined ITS and LSU regions support their placements in Fusicolla and recognition of new species. Fusicolla gigas sp. nov. is characterized by C-shaped, 0−4(−5)-septate microconidia, falcate, (1–)4–9-septate macroconidia up to 80 µm long, and pinkish orange colonies on PDA. Fusicolla guangxiensis sp. nov. is distinguished by long-fusiform, falcate, (0–)1(–3)-septate macroconidia with acute ends, and orange colonies on PDA. Their macroconidia produced on OA are obvious larger than those on PDA. Distinctions between the new species and their close relatives are compared in detail.
... SAmueLS (1985) established the connection between some Chaetopsina asexual morphs with the sexual morphs of four new hypocrealean species assigned to the Nectria episphaeria group in the broad sense, based on similar peridial anatomy. the sexual morphs associated with Chaetopsina asexual morphs were thereafter placed in Cosmospora (roSSmAn et al., 1999;nirenBerG & SAmueLS, 2000;ZhuAnG & ZhAnG, 2002;hoSoYA & tuBAKi, 2004;nonG & ZhuAnG, 2005;hirooKA et al., 2008;roSSmAn et al., 2008). Based on molecular data, a new genus Chaetopsinectria was proposed by Luo & ZhuAnG (2010) to accommodate them. ...
two new species of Chaetopsina are described and illustrated from specimens collected in French Guiana. Based on morphological divergences of both sexual and asexual morphs from known Chaetopsina species as well as phylogenetic analysis of itS and LSu sequences, C. guyanensis and C. saulensis are proposed as new species. in addition, two new combinations in Chaetopsina are proposed. Résumé : deux nouvelles espèces de Chaetopsina sont décrites et illustrées à partir de matériel récolté en Guyane française. en se fondant sur les différences morphologiques des stades sexués et asexués avec les espèces de Chaetopsina connues, ainsi que sur l'analyse phylogénétique des séquences itS et LSu, C. guya-nensis et C. saulensis sont proposées comme espèces nouvelles. en outre, deux nouvelles combinaisons sont proposées et trois espèces sont réintégrées dans le genre Chaetopsina.
... Morphological re-identification. The six strains were re-analyzed morphologically, according to the procedure previously applied by Hosoya and Tubaki [7] for their description of Fusicolla matuoi, a member of the Fusariumlike clade. This involved cultivation on nutrient-rich agar, i.e., potato dextrose agar (PDA), as well as nutrient-poor agar, i.e., synthetic nutrient agar (SNA) with or without fragments of carnation leaf, at 23 o C for 30 days under alternating cycles of light (i.e., day) and dark (i.e., night) conditions. ...
... The predominant occurrence of one-septate macroconidia (Fig. 1H) appears to be discriminatory for the species as opposed to the other members of the Fusarium-like clade, which generally form macroconidia that are three-septate or more, as was seen in our cultures of IHEM 2989 and IHEM 2040 (Table 1, Fig. 2B and 2C). Microconidia were also detected in all SNA cultures of Pseudofusicolla belgica (Fig. 1F), though similar as in Fusicolla matuoi and other Fusicolla spp., these form a continuum in conidial shape and length with the macroconidia [1,7]. Moreover, our Pseudofusicolla belgica strains produced chlamydospores (Fig. 1I). ...
Recently, the Fusarium genus has been narrowed based upon phylogenetic analyses and a Fusarium-like clade was adopted. The few species of the Fusarium-like clade were moved to new, re-installed or existing genera or provisionally retained as "Fusarium." Only a limited number of reference strains and DNA marker sequences are available for this clade and not much is known about its actual species diversity. Here, we report six strains, preserved by the Belgian fungal culture collection BCCM/IHEM as a Fusarium species, that belong to the Fusarium-like clade. They showed a slow growth and produced pionnotes, typical morphological characteristics of many Fusarium-like species. Multilocus sequencing with comparative sequence analyses in GenBank and phylogenetic analyses, using reference sequences of type material, confirmed that they were indeed member of the Fusarium-like clade. One strain was identified as "Fusarium" ciliatum whereas another strain was identified as Fusicolla merismoides. The four remaining strains were shown to represent a unique phylogenetic lineage in the Fusarium-like clade and were also found morphologically distinct from other members of the Fusarium-like clade. Based upon phylogenetic considerations, a new genus, Pseudofusicolla gen. nov., and a new species, Pseudofusicolla belgica sp. nov., were installed for this lineage. A formal description is provided in this study. Additional sampling will be required to gather isolates other than the historical strains presented in the present study as well as to further reveal the actual species diversity in the Fusarium-like clade.
... They occur on fungi, scale insects, leaves, barks, herbaceous and woody substrates and are distributed in tropical to temperate regions. Fiftynine species have been accepted in the genus (Rossman et al. 1999, Nirenberg and Samuels 2000, Zhuang and Zhang 2002, Hosoya and Tubaki 2004, Nong and Zhuang 2005, Hirooka et al. 2008, Rossman et al. 2008. They show significant diversity of morphology, ecology and anamorphic stage. ...
... DISCUSSION Many nectriaceous fungi possess small perithecia (less than 300 mm diam.) and a Cosmospora-like perithecial wall structure (cells of the ascomatal wall surface lacking a definite shape, often with a meandering aspect with walls of variable thickness and narrow lumen, adjacent cells joined by fine pores, and a thin perithecial wall usually fewer than 20 mm). They have been placed in Cosmospora, which resulted in a rapid increase of species number in the genus (Rossman et al. 1999, Nirenberg and Samuels 2000, Zhuang and Zhang 2002, Hosoya and Tubaki 2004, Nong and Zhuang 2005, Hirooka et al. 2008, Rossman et al. 2008. Nevertheless they correlate with several anamorphic genera and appear to be heterogeneous FIG. 1. BI tree inferred from combined ITS and 28S nrDNA datasets. ...
Genus Chaetopsinectria is established to accommodate four species, previously assigned to Cosmospora, that possess Chaetopsina anamorphs, small and red perithecia not collapsing when dry, a typical Cosmospora perithecial wall structure, clavate asci, smooth or striate ascospores, sienna colonies on PDA, and on decaying debris or woody substrates. The segregation of the new genus from Cosmospora is well supported by sequence analyses of nuclear ribosomal DNA (ITS and 28S partial) with maximum parsimony and Bayesian methods. The molecular data indicate that the new genus is distantly related to Cosmospora coccinea Rabenh., the type species of Cosmospora sensu stricto, and its related fungi. Four new combinations, Chaetopsinectria chaetopsinae, C. chaetopsinae-catenulatae, C. chaetopsinae-penicillatae and C. chaetopsinae-polyblastiae, are proposed.
... Rossman et al. (1999) included 49 species in Cosmospora. Cosmospora henanensis Nong & Zhuang (anamorph: Acremonium-like) (Nong and Zhuang 2005) and C. matuoi Hosoya & Tubaki (Fusarium matuoi Hosoya & Tubaki) (Hosoya and Tubaki 2004), both from Asia, have been added. ...
... Twenty species of Cosmospora are known to have Fusarium anamorph Hosoya and Tubaki 2004;Samuels et al. 2006 Mycelium not visible around perithecia or host. Stromata abundantly formed on the stroma of Xylariaceae, "textura epidermoidea" to "textura globulosa." ...
Seven species of the genus Cosmospora collected in Japan are reported in this article. Among them, Cosmospora japonica is described as a new species. Cosmospora henanensis, C. rishbethii, and C. triqua, all of which are known only from their type localities, are added to the Japanese mycobiota. The other species, C. chaetopsinaecatenulatae, C. diminuta, and C. peponum, are new records for Japan. Additional distribution records are given for Cosmospora species hitherto known in Japan.
... They are characterized by small-sized perithecia with glabrous surface or with few to numerous hairs arising from the wall surface, having adjacent perithecial wall cells joined by fine pores, consisting of parallel hyphal elements in papilla, and connected with several anamorphic genera (Rossman et al., 1999). Fifty-six species of the genus are currently accepted, and 15 have been reported from China (Rossman et al., 1999;Fröhlich and Hyde, 2000;Samuels and Nirenberg, 2000;Zhuang and Zhang, 2002;Hosoya and Tubaki, 2004;Nong and Zhuang, 2005). ...
Two new species, Cosmospora gigas and C. cupularis, and their anamorphs are described and illustrated. Their morphological affinities to related taxa are discussed. Phylogenetic relationships of the two new species with some fungi of the Nectriaceae are revealed based on analyses of the ITS1-5.8S-ITS2 and 28S rDNA partial sequences. The results indicate their taxonomic position in Cosmospora, a genus that as currently defined, is not monophyletic.
... Rossman et al. (1999) included 49 species in Cosmospora. Cosmospora henanensis Nong & Zhuang (anamorph: Acremonium-like) (Nong and Zhuang 2005) and C. matuoi Hosoya & Tubaki (Fusarium matuoi Hosoya & Tubaki) (Hosoya and Tubaki 2004), both from Asia, have been added. ...
... Twenty species of Cosmospora are known to have Fusarium anamorph (Rossman et al. 1999;Hosoya and Tubaki 2004;Samuels et al. 2006 Mycelium not visible around perithecia or host. Stromata abundantly formed on the stroma of Xylariaceae, "textura epidermoidea" to "textura globulosa." ...
Seven species of the genus Cosmospora collected in Japan are reported in this article. Among them, Cosmospora japonica is described as a new species. Cosmospora henanensis, C. rishbethii, and C. triqua, all of which are known only from their type localities, are added to the Japanese mycobiota. The other species, C. chaetopsinaecatenulatae, C. diminuta, and C. peponum, are new records for Japan. Additional distribution records are given for Cosmospora species hitherto known in Japan.
Recent publications have argued that there are potentially serious consequences for researchers in recognising distinct genera in the terminal fusarioid clade of the family Nectriaceae. Thus, an alternate hypothesis, namely a very broad concept of the genus Fusarium was proposed. In doing so, however, a significant body of data that supports distinct genera in Nectriaceae based on morphology, biology, and phylogeny is disregarded. A DNA phylogeny based on 19 orthologous protein-coding genes was presented to support a very broad concept of Fusarium at the F1 node in Nectriaceae. Here, we demonstrate that re-analyses of this dataset show that all 19 genes support the F3 node that represents Fusarium sensu stricto as defined by F. sambucinum (sexual morph synonym Gibberella pulicaris). The backbone of the phylogeny is resolved by the concatenated alignment, but only six of the 19 genes fully support the F1 node, representing the broad circumscription of Fusarium. Furthermore, a re-analysis of the concatenated dataset revealed alternate topologies in different phylogenetic algorithms, highlighting the deep divergence and unresolved placement of various Nectriaceae lineages proposed as members of Fusarium. Species of Fusarium s. str. are characterised by Gibberella sexual morphs, asexual morphs with thin- or thick-walled macroconidia that have variously shaped apical and basal cells, and trichothecene mycotoxin production, which separates them from other fusarioid genera. Here we show that the Wollenweber concept of Fusarium presently accounts for 20 segregate genera with clear-cut synapomorphic traits, and that fusarioid macroconidia represent a character that has been gained or lost multiple times throughout Nectriaceae. Thus, the very broad circumscription of Fusarium is blurry and without apparent synapomorphies, and does not include all genera with fusarium-like macroconidia, which are spread throughout Nectriaceae (e.g., Cosmosporella, Macroconia, Microcera). In this study four new genera are introduced, along with 18 new species and 16 new combinations. These names convey information about relationships, morphology, and ecological preference that would otherwise be lost in a broader definition of Fusarium. To assist users to correctly identify fusarioid genera and species, we introduce a new online identification database, Fusarioid-ID, accessible at www.fusarium.org. The database comprises partial sequences from multiple genes commonly used to identify fusarioid taxa (act1, CaM, his3, rpb1, rpb2, tef1, tub2, ITS, and LSU). In this paper, we also present a nomenclator of names that have been introduced in Fusarium up to January 2021 as well as their current status, types, and diagnostic DNA barcode data. In this study, researchers from 46 countries, representing taxonomists, plant pathologists, medical mycologists, quarantine officials, regulatory agencies, and students, strongly support the application and use of a more precisely delimited Fusarium (= Gibberella) concept to accommodate taxa from the robust monophyletic node F3 on the basis of a well-defined and unique combination of morphological and biochemical features. This F3 node includes, among others, species of the F. fujikuroi, F. incarnatum-equiseti, F. oxysporum, and F. sambucinum species complexes, but not species of Bisifusarium [F. dimerum species complex (SC)], Cyanonectria (F. buxicola SC), Geejayessia (F. staphyleae SC), Neocosmospora (F. solani SC) or Rectifusarium (F. ventricosum SC). The present study represents the first step to generating a new online monograph of Fusarium and allied fusarioid genera (www.fusarium.org).
Novel species of fungi described in this study include those from various countries as follows: Angola, Gnomoniopsis angolensis and Pseudopithomyces angolensis on unknown host plants. Australia, Dothiora corym biae on Corymbia citriodora, Neoeucasphaeria eucalypti (incl. Neoeucasphaeria gen. nov.) on Eucalyptus sp., Fumagopsis stellae on Eucalyptus sp., Fusculina eucalyptorum (incl. Fusculinaceae fam. nov.) on Eucalyptus socialis, Harknessia corymbiicola on Corymbia maculata, Neocelosporium eucalypti (incl. Neocelosporium gen. nov., Neocelosporiaceae fam. nov. and Neocelosporiales ord. nov.) on Eucalyptus cyanophylla, Neophaeomoniella corymbiae on Corymbia citriodora, Neophaeomoniella eucalyptigena on Eucalyptus pilularis, Pseudoplagiostoma corymbiicola on Corymbia citriodora, Teratosphaeria gracilis on Eucalyptus gracilis, Zasmidium corymbiae on Corymbia citriodora. Brazil, Calonectria hemileiae on pustules of Hemileia vastatrix formed on leaves of Coffea arabica, Calvatia caatinguensis on soil, Cercospora solanibetacei on Solanum betaceum, Clathrus natalensis on soil, Diaporthe poincianellae on Poincianella pyramidalis, Geastrum piquiriunense on soil, Geosmithia carolliae on wing of Carollia perspicillata, Henningsia resupinata on wood, Penicillium guaibinense from soil, Periconia caespitosa from leaf litter, Pseudocercospora styracina on Styrax sp., Simplicillium filiforme as endophyte from Citrullus lanatus, Thozetella pindobacuensis on leaf litter, Xenosonderhenia coussapoae on Coussapoa floccosa. Canary Islands (Spain), Orbilia amarilla on Euphorbia canariensis. Cape Verde Islands, Xylodon jacobaeus on Eucalyptus camaldulensis. Chile, Colletotrichum arboricola on Fuchsia magellanica. Costa Rica, Lasiosphaeria miniovina on tree branch. Ecuador, Ganoderma chocoense on tree trunk. France, Neofitzroyomyces nerii (incl. Neofitzroyomyces gen. nov.) on Nerium oleander. Ghana, Castanediella tereticornis on Eucalyptus tereticornis, Falcocladium africanum on Eucalyptus brassiana, Rachicladosporium corymbiae on Corymbia citriodora. Hungary, Entoloma silvaefrondosae in Carpinus betulus-Pinus sylvestris mixed forest. Iran, Pseudopyricularia persiana on Cyperus sp. Italy, Inocybe roseascens on soil in mixed forest. Laos, Ophiocordyceps houaynhangensis on Coleoptera larva. Malaysia, Monilochaetes melastomae on Melastoma sp. Mexico, Absidia terrestris from soil. Netherlands, Acaulium pannemaniae, Conioscypha boutwelliae, Fusicolla septimanifiniscientiae, Gibellulopsis simonii, Lasionectria hilhorstii, Lectera nordwiniana, Leptodiscella rintelii, Parasarocladium debruynii and Saro cladium dejongiae (incl. Sarocladiaceae fam. nov.) from soil. New Zealand, Gnomoniopsis rosae on Rosa sp. and Neodevriesia metrosideri on Metrosideros sp. Puerto Rico, Neodevriesia coccolobae on Coccoloba uvifera, Neodevriesia tabebuiae and Alfaria tabebuiae on Tabebuia chrysantha. Russia, Amanita paludosa on bogged soil in mixed deciduous forest, Entoloma tiliae in forest of Tilia × europaea, Kwoniella endophytica on Pyrus communis. South Africa, Coniella diospyri on Diospyros mespiliformis, Neomelanconiella combreti (incl. Neomelanconiellaceaefam. nov. and Neomelanconiella gen. nov.) on Combretum sp., Polyphialoseptoria natalensis on unidentified plant host, Pseudorobillarda bolusanthi on Bolusanthus speciosus, Thelonectria pelargonii on Pelargonium sp. Spain, Vermiculariopsiella lauracearum and Anungitopsis lauri on Laurus novocanariensis, Geosmithia xerotolerans from a darkened wall of a house, Pseudopenidiella gallaica on leaf litter. Thailand, Corynespora thailandica on wood, Lareunionomyces loeiensis on leaf litter, Neocochlearomyces chromolaenae (incl. Neocochlearomyces gen. nov.) on Chromolaena odorata, Neomyrmecridium septatum (incl. Neomyrmecridium gen. nov.), Pararamichloridium caricicola on Carex sp., Xenodactylaria thailandica (incl. Xenodactylariaceae fam. nov. and Xenodactylaria gen. nov.), Neomyrmecridium asiaticum and Cymostachys thailandica from unidentified vine. USA, Carolinigaster bonitoi (incl. Carolinigaster gen. nov.) from soil, Penicillium fortuitum from house dust, Phaeotheca shathenatiana (incl. Phaeothecaceae fam. nov.) from twig and cone litter, Pythium wohlseniorum from stream water, Superstratomyces tardicrescens from human eye, Talaromyces iowaense from office air. Vietnam, Fistulinella olivaceoalba on soil. Morphological and culture characteristics along with DNA barcodes are provided.
