Fig 1- - uploaded by Halil Erhan Eroğlu
Content may be subject to copyright.
Micronucleus, mitotic and replication indexes changes of Helichrysum plicatum subsp. plicatum H. plicatum subsp. plicatum induced MN, decreased MI and RI in human lymphocytes. These increases and decreases were dose-dependent. Increase of MN was clear for 1 mg/mL concentration. Decreases of MI and RI were 0.5 and 1 mg/mL concentrations. A negative correlation was observed between MN induction and cell proliferation; the higher the MN frequency detected in exposed individuals, the lower the values of nuclear division progression expressed as RI.
Source publication
Helichrysum plicatum DC. subsp. plicatum (Asteraceae), (common name; everlasting) is a medicinal plant and natural product. For centuries, it has been used as tea in Turkey because of its medicinal properties. There are few reports in literature about genotoxic effects of this plant. The aim of this work is to evaluate the genotoxic and antimitotic...
Contexts in source publication
Context 1
... tum exhibit genotoxic properties as well as an- timitotic properties. A negative correlation was observed between MN induction and cell pro- liferation; namely the higher the MN frequency were detected in exposed individuals, the lower the values of nuclear division progression were expressed as MI and RI (Figure 1). ...
Context 2
... the present study indicate that Helichrysum plicatum subsp. plicatum extracts used in alter- native medicine induced MN, decreased MI and RI in human lymphocytes (Figure 1). We are of the opinion that it should not be used in high quantities in the general population because of its genotoxic and cytotoxic properties. ...
Similar publications
Helichrysum Mill., belonging to the family Asteraceae, is commonly known as "everlasting". Helichrysum species have been used as herbal tea for centuries in Turkey against gallbladder disorders because of their bile regulatory and diuretic properties. However, there are few reports in the literature regarding the genotoxic effects of these plants....
Citations
... plicatum'un toprak üstü kısmından hazırlanan dekoksiyonun genotoksik ve antimitotik aktivitesi incelenmiştir. Ekstre 0,01; 0,05; 0,10; 0,50 ve 1,00 mg/ml konsantrasyonlarda sitokin blok mikronükleus (CBMN) ve mitotik indeks (Mİ) testlerine tabi tutulmuş ve sonuç olarak 1,00 mg/ml konsantrasyonunda ekstrenin belirgin şekilde kromozomal hasara neden olarak mikronükleus oluşumunu indüklediği, kontrol grubuna kıyasla da hücre bölünmesinde gecikmeye neden olarak mitotik ve replikasyon indekslerini azalttığı görülmüştür (42). ...
... It is also used traditionally for the treatment of coronary heart (Aggarwal 2009;Balasundram et al. 2006;Czinner et al. 2001;George et al. 2009). Although H. arenarium is highly effective in conditions such as blood glucose level, atherosclerotic disease, lower respiratory tract infection, antimicrobial effects, there are studies showing that it is effective in inducing chromosomal damage in high concentrations (Morikawa et al. 2009(Morikawa et al. , 2015Eroğlu et al. 2010;Stanojković et al. 2010;Gradinaru et al. 2014;Mao et al. 2017;Akin and Saki 2019;Lazarević et al. 2019). These properties of H. arenarium are closely related to flavonoids, essential oils and phenolic content. ...
... Studies have been conducted on the effects of plant growth regulators (Altuntaş et al. 2012), pesticides (Dubovskiy et al. 2013;Kurt and Kayış 2015;Er et al. 2017), various heavy metals (Wu and Yi 2015) and various pathogens ( According to our results, H. arenarium extract at 0.25% and 0.5% doses caused an increase in the number of hemocytes. Eroğlu et al. (2010) showed that micronuclei, Mitotic Indicies, Replication Index values in the samples where H. plicatum subsp. plicatum extract was applied in low doses were similar to the control group. ...
... Our results are consistent with this study. Eroğlu et al. (2010) found that Mitotic Indicies and Replication Index values showed a significant decrease in the samples given 1 mg ml −1 H. plicatum subsp. plicatum extract compared to the control group, but there was a significant increase in the formation of micronuclei with the same dose. ...
Helichrysum arenarium (L.) (Asterales:Asteraceae) Moench is a therapeutic plant which contains etheric oil, flavones and flavon glycosides, sterins, bitter substances and tannins having various coumarins. This plant is thought to have important characteristics such as diuretic effect, dropping stones and sand from the kidney, regulating digestive disorders, strengthening the immune system, and having antibiotic and antioxidant effects. Additionally, this plant is traditionally used in liver and biliary tract diseases and also shows anti-inflammatory and detoxifying properties. Model organism Galleria mellonella L. (Lepidoptera:Pyralidae) is an invertebrate species that is frequently used to study the effects of human pathogens and various pesticides, hormones, etc. on immune system. In our study, we examined the effect of various doses of H. arenarium on the hemocyte count and behavior of G. mellonella larvae. According to the findings obtained at the end of our study, H. arenarium caused an increase in hemocyte count with the injection of 0.25% and 0.5% doses compared to the untreated and DMSO groups. At the same time, the 0.25% and 0.5% doses showed a strong encapsulation-melanization response and an increase in phenoloxidase enzyme activity over 24 h compared to the other injected groups. Based on these results, H. arenarium extract has an anti-mitotic activity at high doses (above 0.5%). This effect may be due to the fact that the plant extract supports mitosis at a certain dose, while being toxic when exceeding it.
... Potential anticancer activity might not result from cytotoxic or induction of apoptosis in the treated cells but the antiproliferative effects of the extracts could be mediated through cell cycle arrest in the S-phase (González-Sarrías et al. 2012). Thus, mitotic and replication indexes (MI and RI, respectively) are used as biomarkers for an adequate cell proliferation (Eroglu et al. 2010). ...
We examined the effect of water extracts of Persea americana fruit, and of the leaves of Tabernamontana divericata, Nerium oleander and Annona cherimolia (positive control) on Vicia faba root cells. We had confirmed in our previously published data the cytotoxicity of these plant extracts on four human cancer cell lines: liver (HepG-2), lung (A549), colon (HT-29) and breast (MCF-7). Vicia faba roots were soaked in plant extracts at dilutions of 100, 1,250, 2,500, 5,000, 10,000, 20,000 ppm for 4 and 24 h. All treatments resulted in a significant reduction in the mitotic index in a dose dependant manner. Root cells treated with T. divericata, N. oleander and A. cherimolia exhibited a decrease in prophase cell percentage, increase in micronuclei and chromosomal abnormalities as concentration increased. The P. americana treatment showed the highest cytotoxic effect on cancer cells, prophase cell percentage increased linearly with the applied concentration and no micronuclei were detected. This study shows that root tip assay of beans can be used in initial screening for new plant extracts to validate their use as candidates for containing active cytotoxic agents against malignant cells. This will greatly help in exploring new plant extracts as drugs for cancer treatment.
Helichrysum genus, known as everlasting flowers or immortelles worlwide, is a member of the Asteraceae family represented by about 600 species over the world. Helichrysum plicatum DC., which is part of this genus, is an important figure in Turkey, Balkan Penunsula, and Iran because it is traditionally used for treatment of many diseases, especially diabetes, kidney stone, jaundice, and wounds. It is of great importance for Turkey due to hosting four subspecies of Helichrysum plicatum DC., which are Helichrysum plicatum DC. subsp. plicatum DC., Helichrysum plicatum DC. subsp. pseudoplicatum (Nab.) Davis & Kubicha, Helichrysum plicatum DC. subsp. polyphyllum (Lebed) Davis & Kubicha, and Helichrysum plicatum DC. subsp. isauricum Parolly. Flavonoids, pyrones, phloroglucinols, terpenoid, phtalides, and phenolic acids were detected in Helichrysum plicatum with a small number of studies. Likewise, monoterpenes, fatty acids, and sesquiterpenes were found in essential oil with limited number of phytochemical investigations. In recent year, the several pharmacological activities such as antioxidant, antimicrobial, antifungal, antiinflammaory, antidiabetic, antiurolithiatic, insecticidal, and anticancer activity have been confirmed by researchers. In this chapter, it is aimed that its traditional uses, phytochemistry and pharmacological properties of Helichrysum plicatum until date will be summarized and highlighted to explore the gaps and to contribute to future potential.Keywords
Helichrysum plicatum
AsteraceaeHelichrysum plicatum subsp. polyphyllumHelichrysum plicatum subsp. plicatumHelichrysum plicatum subsp. isauricumHelichrysum plicatum subsp. pseudoplicatumPharmacognosyPhytoconstituentsEthnopharmacology
Wound is tissue damage that occurs in the skin. Helichrysum species (Altınotu) are rich in phenolic compounds used in traditional medicine for wound healing. The main component in their flower head (capitulum) is phenolic compounds. The present study investigates the proliferative, oxidative stress, and wound healing properties of the methanolic extract of Helichrysum plicatum subsp. pseudoplicatum capitulum on a human dermal fibroblast (HDF) cell line in this study. H plicatum subsp. pseudoplicatum capitulums were collected in Erzurum, Turkey (altitude 1950 m), dried, pulverized, and extracted with methanol. Firstly, total phenolic contents were determined and secondly, the proliferative effect, oxidative stress activities, and wound healing effects on HDF cells were evaluated by the cell proliferation kit (XTT) test, total antioxidant status (TAS), and total oxidant status (TOS) commercial kits, and the scratch experiment by taking microscopic images of the cells at 0, 12, 18, and 24 h, respectively. Total phenolic content was found to be 142.00 ± 0.73 mg gallic acid equivalent per gram (GAE/g) extract. The capitulum extract has a proliferative effect at 0.5 to 10 µg/mL concentrations according to the XTT test results. It was observed that TAS levels significantly increased in the plant extract at the concentration ranges 1 to 10 µg/mL ( P < .01). About 1 to 5 µg/mL plant extract started to increase cell migration at the 12 h and significantly closed the wound area at the 24 h. At the doses between 1 to 5 μg/mL, it has the most substantial effect on both cell viability and antioxidant effect, and wound healing was found to be in this concentration range. These findings suggested that the H plicatum subsp. pseudoplicatum capitulum is a valuable source of phenolic content with important antioxidant activity at wound healing and it was concluded that the capitulum extract accelerates wound healing by increasing cell migration in low doses.
