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Median lobe of the aedeagus of Pterostichus adstrictus from the Taschachtal (Ötztal Alps, Austria), (a) right lateral view, (b) left lateral view, scale bar = 1 mm.
Source publication
The last ice age considerably influenced distribution patterns of extant species of plants and animals, with some of them now inhabiting disjunct areas in the subarctic/arctic and alpine regions. This arctic-alpine distribution is characteristic for many cold-adapted species with a limited dispersal ability and can be found in many invertebrate tax...
Contexts in source publication
Context 1
... particularly applied to the tip, which in the ventral view was sharply tapered. The internal sac also showed typical structures, although sclerites were missing (Figures 2 and 3a). ...
Context 2
... particularly applied to the tip, which in the ventral view was sharply tapered. The internal sac also showed typical structures, although sclerites were missing (Figures 2 and 3a). ...
Citations
... However, further examination of their morphology showed only subtle differences between our specimen and the holotype of Aspilota umbrosa, consistent with a distance of 8600 km between collection sites, and not enough to justify the establishment of a new species. Moreover, minimal morphological differences are known to occur in other insects with boreo-alpine distributions (Paill et al., 2021). We contemplated the possibility of conducting a barcoding analysis as an additional source of information, but none of the authors involved had the possibility to perform it in the recently captured specimen, or the holotype of Aspilota umbrosa stored in Russia. ...
The species Aspilota umbrosa Belokobylskij, 2007 is recorded for the first time in Europe. The first photographsof this species, both of the holotype and of the new record, the second in the world, are provided. The holotype was foundnear the Klyuchevskaya Sopka volcano (Kamchatka peninsula, Russia). The new specimen of this species was collectedin a superficial subterranean habitat (the MSS – Milieu Souterrain Superficiel) in the rock glacier in Val Sassa (SwissNational Park, Grisons, Switzerland) by a subterranean trap in 2020. This is the first record of A. umbrosa in the MSS ofthe Swiss Alps and in a rock glacier. A key to the known Swiss species of Aspilota is provided
... Замечания. Бореальный трансголарктический вид; недавно обнаружен в Австрийских Альпах (Paill et al., 2021). Указывался из парка ранее (Будилов, 2013). ...
An annotated list of 123 species from 36 genera, 21 tribes and 10 subfamilies of Carabidae collected in the Anyuy National Park (Khabarovsky krai, Russia) is given. Fore species, Bembidion lissonotum, B. lucillum, B. shimoyamai, and Tachyura exarata, are recorded from Khabarovsky krai for the first time and 65 species are new for the fauna of Anyuy Park.
... Замечания. Бореальный трансголарктический вид; недавно обнаружен в Австрийских Альпах (Paill et al., 2021). Указывался из парка ранее (Будилов, 2013). ...
Приводится список 123 видов из 36 родов, 21 трибы и 10 подсемейств Carabidae, выявленных на территории национального парка «Анюйский». Впервые для фауны Хабаровского края указываются Bembidion lissonotum, B. lucillum, B. shimoyamai и Tachyura exarata, а для национального парка «Анюйский» впервые приводятся 65 видов.
An annotated list of 123 species from 36 genera, 21 tribes and 10 subfamilies of Carabidae collected in the Anyuy National Park (Khabarovsky krai, Russia) is given. Fore species, Bembidion lissonotum, B. lucillum, B. shimoyamai, and Tachyura exarata, are recorded from Khabarovsky krai for the first time and 65 species are new for the fauna of Anyuy Park.
... However, current research has shown the limitation of using DNA sequences for species identification if species pairs have very recent origins or hybridize [66]. Such patterns were shown for two pairs of closely related Pterostichus species: no significant sequence divergence within three nuclear markers and mtDNA for P. nigrita and P. rhaeticus were revealed [66], and similarly a lack of mitochondrial divergence between P. oblongopunctatus and northern populations of P. adstrictus was detected [67]. In this case, next-generation sequencing (NGS) methods were more informative [68], and the restriction site-associated DNA sequencing method (RAD-seq) was more informative [62,[68][69][70][71]. ...
The geographic patterns of genetic and morphological variability in ground beetles were examined throughout Northern Eurasia and North America using the most abundant circumpolar tundra subspecies, Pterostichus (Cryobius) brevicornis brevicornis (Kirby, 1837), as a model. Phylogenetic structure was assessed on the basis of a Bayesian approach using two DNA markers (partial sequences of the COI and 28S rRNA genes), while phylogeographic patterns and population genetic diversity were estimated using the COI gene only. Morphological patterns were analysed using elliptical Fourier coefficients that were calculated based on the pronotum and male genitalia shape outlines. The subspecies shares 23 COI haplotypes throughout its entire circumpolar range, while eight haplotypes of 28S rRNA were detected in Northern Eurasia. Phylogenetic analysis did not reveal subdivided species lineages with strict geographical imprint. The network, FST and uncorrected pairwise divergence analyses showed that the genetic distances between populations increase by longitude from Northeastern Asia to Europe. The genetic variability among the five studied geographical population groups of P. b. brevicornis was relatively high. The MANOVA showed significant regional divergence between local populations in Northern Eurasia based on both morphological markers, but only male genitalia variability was geographically structured. Neither the pronotum shape nor the male genitalia shape aligned with the phylogeographic patterns discovered on the basis of COI sequences. The genetic (COI) marker had more variation within, rather than among, population groups in addition to morphology of pronotum but not male genitalia.
... All these approaches, however, rely highly on comprehensive on-line reference libraries of correctly identified specimens (e.g., Brandon-Mong et al. 2015;Creer et al. 2016;Staats et al. 2016). Ideally, such libraries include sequence data of a species` complete distribution range that can provide additional information of phylogeographic substructures that are well-known for many species (e.g., Gentile et al. 2010;Raupach et al. 2014;Paill et al. 2021). ...
DNA barcoding has become the most popular approach for species identification in recent years. As part of the German Barcode of Life project, the first DNA barcode library for terrestrial and freshwater isopods from Germany is presented. The analyzed barcode library included 38 terrestrial (78% of the documented species of Germany) and five freshwater (63%) species. A total of 513 new barcodes was generated and 518 DNA barcodes were analyzed. This analysis revealed surprisingly high intraspecific genetic distances for numerous species, with a maximum of 29.4% for Platyarthrus hoffmannseggii Brandt, 1833. The number of BINs per species ranged from one (32 species, 68%) to a maximum of six for Trachelipus rathkii (Brandt, 1833). In spite of such high intraspecific variability, interspecific distances with values between 12.6% and 29.8% allowed a valid species assignment of all analyzed isopods. The observed high intraspecific distances presumably result from phylogeographic events, Wolbachia infections, atypical mitochondrial DNAs, heteroplasmy, or various combinations of these factors. Our study represents the first step in generating an extensive reference library of DNA barcodes for terrestrial and freshwater isopods for future molecular biodiversity assessment studies.
... Since DNA barcoding was introduced as a method for biological species discrimination (Hebert, Ratnasingham & De Waard, 2003), several studies have shown that its delimiting powers also apply to various insect groups (e.g., Raupach et al., 2016;Huemer et al., 2019;Zangl et al., 2019;Galimberti et al., 2020). However, DNA barcoding also has well known limitations with respect to recently diverged species, large population sizes retaining divergent haplotypes and hybridization/introgression (e.g., Van Velzen et al., 2012;Ermakov et al., 2015;Cong et al., 2017;Zangl et al., 2020;Paill et al., 2021), and species delimitation therefore benefits from additional sources of data (e.g., Trewick, 2008;Liu et al., 2017). Conducted in the framework of the Austrian Barcode of Life initiative (ABOL, http://www.abol.ac.at;Haring, ...
Background
Snow scorpionflies (genus Boreus ) belong to a family of Mecoptera, Boreidae, that has been vastly neglected by entomological researchers due to their shift in seasonality to the winter months. Their activity during this time is regarded as a strategy for predator avoidance and regular sightings on snow fields suggest that this also facilitates dispersal. However, many aspects about snow scorpionflies, especially systematics, taxonomy, distribution of species, phylogenetics and phylogeography have remained fairly unexplored until today. In this study, we fill some of these gaps by generating a reference DNA barcode database for Austrian snow scorpionflies in the frame of the Austrian Barcode of Life initiative and by characterising morphological diversity in the study region.
Methods
Initial species assignment of all 67 specimens was based on male morphological characters previously reported to differ between Boreus species and, for females, the shape of the ovipositor. DNA barcoding of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene was carried out for all 67 samples and served as a basis for BIN assignment, genetic distance calculations, as well as alternative species delimitation analyses (ABGD, GMYC, bGMYC, bPTP) and a statistical parsimony network to infer phylogenetic relationships among individual samples/sampling sites.
Results
Morphological investigations suggested the presence of both Boreus hyemalis and Boreus westwoodi in Austria. DNA barcoding also separated the two species, but resulted in several divergent clades, the paraphyly of B. westwoodi in Austria, and high levels of phylogeographic structure on a small geographic scale. Even though the different molecular species delimitation methods disagreed on the exact number of species, they unequivocally suggested the presence of more than the traditionally recognized two Boreus species in Austria, thus indicating potential cryptic species within the genus Boreus in general and especially in B. westwoodi .
The list includes the results of identification of 574 specimens collected around Khabarovsk, which belong to 117 species in 32 genera. Six species: Amara solskyi, Bembidion morawitzi, B. pogonoides, Chlaenius spoliatus, Dyschirius yezoensis, and Elaphrus comatus are newly recorded from Khabarovsky Region. The records of four species from this area — Chaetodera laetescripta, Amara pallidula, A. gigantea, and Chlaenius alutaceus — are supported by additional material.
An annotated list of 48 carabid beetle species belonging to 16 genera of 13 tribes in 7 subfamilies from Bolshoi Shantar Island is given. Two species are listed according to the literature data for the Shantar Archipelago as a whole; 39 species are recorded for the fi rst time for the Shantar Islands, and 4 species (Notiophilus semistriatus,
Bembidion hyperboraeorum, Pterostichus diligens, and Agonum exaratum), for Khabarovsk Territory. The carabid fauna of Bolshoi Shantar Island is mainly formed by widely distributed trans-Holarctic (35.4% of the total number of the species), trans-Palaearctic (35.4%), and Eastern Palaearctic (25.0%) species of the boreal complex which comprises the boreal (60.4%), Arcto-boreal (6.3%), subboreal (18.8%), and temperate (12.5%) species. The fauna is peculiar due to the presence of the narrowly distributed Carabus hummeli middendorfi i and, probably, also Pterostichus (Cryobius) sp., but these taxa require further study. The distribution of the species given in the Catalogue of Palaearctic Coleoptera (2017) is clarifi ed. The reliability of the geographical labels to A.I. Sycheva’s material deposited in the
collection of the Zoological Institute, Russian Academy of Sciences, St. Petersburg, is discussed.
